Virulence-related Mycobacterium avium subsp hominissuis MAV-2928 gene is associated with vacuole remodeling in macrophages
Authors
Jha, S.S.Danelishvili, L.
Wagner, D.
Maser, J.
Li, Y.-J.
Morić, Ivana
Vogt, S.
Yamazaki, Y.
Lai, B.
Bermudez, L.E.
Article (Published version)
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Background: Mycobacterium avium subsp hominissuis (previously Mycobacterium avium subsp avium) is an environmental organism associated with opportunistic infections in humans. Mycobacterium hominissuis infects and replicates within mononuclear phagocytes. Previous study characterized an attenuated mutant in which the PPE gene (MAV-2928) homologous to Rv1787 was inactivated. This mutant, in contrast to the wild-type bacterium, was shown both to have impaired the ability to replicate within macrophages and to have prevented phagosome/lysosome fusion. Results: MAV-2928 gene is primarily upregulated upon phagocytosis. The transcriptional profile of macrophages infected with the wild-type bacterium and the mutant were examined using DNA microarray, which showed that the two bacteria interact uniquely with mononuclear phagocytes. Based on the results, it was hypothesized that the phagosome environment and vacuole membrane of the wild-type bacterium might differ from the mutant. Wild-type bac...terium phagosomes expressed a number of proteins different from those infected with the mutant. Proteins on the phagosomes were confirmed by fluorescence microscopy and Western blot. The environment in the phagosome of macrophages infected with the mutant differed from the environment of vacuoles with M. hominissuis wild-type in the concentration of zinc, manganese, calcium and potassium. Conclusion: The results suggest that the MAV-2928 gene/operon might participate in the establishment of bacterial intracellular environment in macrophages.
Source:
BMC Microbiology, 2010, 10Publisher:
- BMC
Funding / projects:
- We are grateful for the support of the Mass Spectrometry Core Facility of the Environmental Health Sciences Center, Oregon State University, and from grant number P30 ES00210, from National Institute of Environmental Health Sciences, National Institutes of Health. This work was also supported by the NIH grants # AI47010 and AI043199. We thank Denny Weber for help in preparing the manuscript.
DOI: 10.1186/1471-2180-10-100
ISSN: 1471-2180
PubMed: 20359357
Scopus: 2-s2.0-77950522924
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Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Jha, S.S. AU - Danelishvili, L. AU - Wagner, D. AU - Maser, J. AU - Li, Y.-J. AU - Morić, Ivana AU - Vogt, S. AU - Yamazaki, Y. AU - Lai, B. AU - Bermudez, L.E. PY - 2010 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/469 AB - Background: Mycobacterium avium subsp hominissuis (previously Mycobacterium avium subsp avium) is an environmental organism associated with opportunistic infections in humans. Mycobacterium hominissuis infects and replicates within mononuclear phagocytes. Previous study characterized an attenuated mutant in which the PPE gene (MAV-2928) homologous to Rv1787 was inactivated. This mutant, in contrast to the wild-type bacterium, was shown both to have impaired the ability to replicate within macrophages and to have prevented phagosome/lysosome fusion. Results: MAV-2928 gene is primarily upregulated upon phagocytosis. The transcriptional profile of macrophages infected with the wild-type bacterium and the mutant were examined using DNA microarray, which showed that the two bacteria interact uniquely with mononuclear phagocytes. Based on the results, it was hypothesized that the phagosome environment and vacuole membrane of the wild-type bacterium might differ from the mutant. Wild-type bacterium phagosomes expressed a number of proteins different from those infected with the mutant. Proteins on the phagosomes were confirmed by fluorescence microscopy and Western blot. The environment in the phagosome of macrophages infected with the mutant differed from the environment of vacuoles with M. hominissuis wild-type in the concentration of zinc, manganese, calcium and potassium. Conclusion: The results suggest that the MAV-2928 gene/operon might participate in the establishment of bacterial intracellular environment in macrophages. PB - BMC T2 - BMC Microbiology T1 - Virulence-related Mycobacterium avium subsp hominissuis MAV-2928 gene is associated with vacuole remodeling in macrophages VL - 10 DO - 10.1186/1471-2180-10-100 ER -
@article{ author = "Jha, S.S. and Danelishvili, L. and Wagner, D. and Maser, J. and Li, Y.-J. and Morić, Ivana and Vogt, S. and Yamazaki, Y. and Lai, B. and Bermudez, L.E.", year = "2010", abstract = "Background: Mycobacterium avium subsp hominissuis (previously Mycobacterium avium subsp avium) is an environmental organism associated with opportunistic infections in humans. Mycobacterium hominissuis infects and replicates within mononuclear phagocytes. Previous study characterized an attenuated mutant in which the PPE gene (MAV-2928) homologous to Rv1787 was inactivated. This mutant, in contrast to the wild-type bacterium, was shown both to have impaired the ability to replicate within macrophages and to have prevented phagosome/lysosome fusion. Results: MAV-2928 gene is primarily upregulated upon phagocytosis. The transcriptional profile of macrophages infected with the wild-type bacterium and the mutant were examined using DNA microarray, which showed that the two bacteria interact uniquely with mononuclear phagocytes. Based on the results, it was hypothesized that the phagosome environment and vacuole membrane of the wild-type bacterium might differ from the mutant. Wild-type bacterium phagosomes expressed a number of proteins different from those infected with the mutant. Proteins on the phagosomes were confirmed by fluorescence microscopy and Western blot. The environment in the phagosome of macrophages infected with the mutant differed from the environment of vacuoles with M. hominissuis wild-type in the concentration of zinc, manganese, calcium and potassium. Conclusion: The results suggest that the MAV-2928 gene/operon might participate in the establishment of bacterial intracellular environment in macrophages.", publisher = "BMC", journal = "BMC Microbiology", title = "Virulence-related Mycobacterium avium subsp hominissuis MAV-2928 gene is associated with vacuole remodeling in macrophages", volume = "10", doi = "10.1186/1471-2180-10-100" }
Jha, S.S., Danelishvili, L., Wagner, D., Maser, J., Li, Y.-J., Morić, I., Vogt, S., Yamazaki, Y., Lai, B.,& Bermudez, L.E.. (2010). Virulence-related Mycobacterium avium subsp hominissuis MAV-2928 gene is associated with vacuole remodeling in macrophages. in BMC Microbiology BMC., 10. https://doi.org/10.1186/1471-2180-10-100
Jha S, Danelishvili L, Wagner D, Maser J, Li Y, Morić I, Vogt S, Yamazaki Y, Lai B, Bermudez L. Virulence-related Mycobacterium avium subsp hominissuis MAV-2928 gene is associated with vacuole remodeling in macrophages. in BMC Microbiology. 2010;10. doi:10.1186/1471-2180-10-100 .
Jha, S.S., Danelishvili, L., Wagner, D., Maser, J., Li, Y.-J., Morić, Ivana, Vogt, S., Yamazaki, Y., Lai, B., Bermudez, L.E., "Virulence-related Mycobacterium avium subsp hominissuis MAV-2928 gene is associated with vacuole remodeling in macrophages" in BMC Microbiology, 10 (2010), https://doi.org/10.1186/1471-2180-10-100 . .