GABAergic signaling in primary lens epithelial and lentoid cells and its involvement in intracellular Ca2+ modulation
Само за регистроване кориснике
2011
Аутори
Schwirtlich, MarijaKwakowsky, Andrea
Emri, Zsuzsa
Antal, Karoly
Lacza, Zsombor
Cselenyak, Attila
Katarova, Zoya
Szabo, Gabor
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Primary lens epithelial cell (LEC) cultures derived from newborn (P0) and one-month-old (P30) mouse lenses were used to study GABA (gamma-aminobutyric acid) signaling expression and its effect on the intracellular Ca2+ ([Ca2+](i)) level. We have found that these cultures express specific cellular markers for lens epithelial and fiber cells, all components of the functional GABA signaling pathway and GABA, thus recapitulating the developmental program of the ocular lens. Activation of both GABA-A and GABA-B receptors (GABA(A)R and GABA(B)R) with the specific agonists muscimol and baclofen, respectively induces [Ca2+](i) transients that could be blocked by the specific antagonists bicuculline and CGP55845 and were dependent on extracellular Ca2+. Bicuculline did not change the GABA-evoked Ca2+ responses in Ca-2-containing buffers, but suppressed them significantly in Ca2+-free buffers suggesting the two receptors couple to convergent Ca2+ mobilization mechanisms with different extracellu...lar Ca2+ sensitivity. Prolonged activation of GABA(B)R induced wave propagation of the Ca2+ signal and persistent oscillations. The number of cells reacting to GABA or GABA + bicuculline in P30 mouse LEC cultures expressing predominantly the synaptic type GABA(A)R did not differ significantly from the number of reacting cells in PO mouse LEC cultures. The GABA-induced Ca2+ transients in P30 (but not P0) mouse LEC could be entirely suppressed by co-application of bicuculline and CGP55845. The GABA-mediated Ca2+ signaling may be involved in a variety of Ca2+-dependent cellular processes during lens growth and epithelial cell differentiation.
Кључне речи:
Neurotransmitters / Lens / LEC / Intracellular calcium / GAD / GABA signaling in development / GABA receptors / Cell differentiationИзвор:
Cell Calcium, 2011, 50, 4, 381-392Издавач:
- Elsevier Sci Ltd, Oxford
Финансирање / пројекти:
- Hungarian National Grant Agency (OTKA) [T038098]
DOI: 10.1016/j.ceca.2011.07.002
ISSN: 0143-4160
WoS: 000296683800007
Scopus: 2-s2.0-80053298031
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Schwirtlich, Marija AU - Kwakowsky, Andrea AU - Emri, Zsuzsa AU - Antal, Karoly AU - Lacza, Zsombor AU - Cselenyak, Attila AU - Katarova, Zoya AU - Szabo, Gabor PY - 2011 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/490 AB - Primary lens epithelial cell (LEC) cultures derived from newborn (P0) and one-month-old (P30) mouse lenses were used to study GABA (gamma-aminobutyric acid) signaling expression and its effect on the intracellular Ca2+ ([Ca2+](i)) level. We have found that these cultures express specific cellular markers for lens epithelial and fiber cells, all components of the functional GABA signaling pathway and GABA, thus recapitulating the developmental program of the ocular lens. Activation of both GABA-A and GABA-B receptors (GABA(A)R and GABA(B)R) with the specific agonists muscimol and baclofen, respectively induces [Ca2+](i) transients that could be blocked by the specific antagonists bicuculline and CGP55845 and were dependent on extracellular Ca2+. Bicuculline did not change the GABA-evoked Ca2+ responses in Ca-2-containing buffers, but suppressed them significantly in Ca2+-free buffers suggesting the two receptors couple to convergent Ca2+ mobilization mechanisms with different extracellular Ca2+ sensitivity. Prolonged activation of GABA(B)R induced wave propagation of the Ca2+ signal and persistent oscillations. The number of cells reacting to GABA or GABA + bicuculline in P30 mouse LEC cultures expressing predominantly the synaptic type GABA(A)R did not differ significantly from the number of reacting cells in PO mouse LEC cultures. The GABA-induced Ca2+ transients in P30 (but not P0) mouse LEC could be entirely suppressed by co-application of bicuculline and CGP55845. The GABA-mediated Ca2+ signaling may be involved in a variety of Ca2+-dependent cellular processes during lens growth and epithelial cell differentiation. PB - Elsevier Sci Ltd, Oxford T2 - Cell Calcium T1 - GABAergic signaling in primary lens epithelial and lentoid cells and its involvement in intracellular Ca2+ modulation EP - 392 IS - 4 SP - 381 VL - 50 DO - 10.1016/j.ceca.2011.07.002 ER -
@article{ author = "Schwirtlich, Marija and Kwakowsky, Andrea and Emri, Zsuzsa and Antal, Karoly and Lacza, Zsombor and Cselenyak, Attila and Katarova, Zoya and Szabo, Gabor", year = "2011", abstract = "Primary lens epithelial cell (LEC) cultures derived from newborn (P0) and one-month-old (P30) mouse lenses were used to study GABA (gamma-aminobutyric acid) signaling expression and its effect on the intracellular Ca2+ ([Ca2+](i)) level. We have found that these cultures express specific cellular markers for lens epithelial and fiber cells, all components of the functional GABA signaling pathway and GABA, thus recapitulating the developmental program of the ocular lens. Activation of both GABA-A and GABA-B receptors (GABA(A)R and GABA(B)R) with the specific agonists muscimol and baclofen, respectively induces [Ca2+](i) transients that could be blocked by the specific antagonists bicuculline and CGP55845 and were dependent on extracellular Ca2+. Bicuculline did not change the GABA-evoked Ca2+ responses in Ca-2-containing buffers, but suppressed them significantly in Ca2+-free buffers suggesting the two receptors couple to convergent Ca2+ mobilization mechanisms with different extracellular Ca2+ sensitivity. Prolonged activation of GABA(B)R induced wave propagation of the Ca2+ signal and persistent oscillations. The number of cells reacting to GABA or GABA + bicuculline in P30 mouse LEC cultures expressing predominantly the synaptic type GABA(A)R did not differ significantly from the number of reacting cells in PO mouse LEC cultures. The GABA-induced Ca2+ transients in P30 (but not P0) mouse LEC could be entirely suppressed by co-application of bicuculline and CGP55845. The GABA-mediated Ca2+ signaling may be involved in a variety of Ca2+-dependent cellular processes during lens growth and epithelial cell differentiation.", publisher = "Elsevier Sci Ltd, Oxford", journal = "Cell Calcium", title = "GABAergic signaling in primary lens epithelial and lentoid cells and its involvement in intracellular Ca2+ modulation", pages = "392-381", number = "4", volume = "50", doi = "10.1016/j.ceca.2011.07.002" }
Schwirtlich, M., Kwakowsky, A., Emri, Z., Antal, K., Lacza, Z., Cselenyak, A., Katarova, Z.,& Szabo, G.. (2011). GABAergic signaling in primary lens epithelial and lentoid cells and its involvement in intracellular Ca2+ modulation. in Cell Calcium Elsevier Sci Ltd, Oxford., 50(4), 381-392. https://doi.org/10.1016/j.ceca.2011.07.002
Schwirtlich M, Kwakowsky A, Emri Z, Antal K, Lacza Z, Cselenyak A, Katarova Z, Szabo G. GABAergic signaling in primary lens epithelial and lentoid cells and its involvement in intracellular Ca2+ modulation. in Cell Calcium. 2011;50(4):381-392. doi:10.1016/j.ceca.2011.07.002 .
Schwirtlich, Marija, Kwakowsky, Andrea, Emri, Zsuzsa, Antal, Karoly, Lacza, Zsombor, Cselenyak, Attila, Katarova, Zoya, Szabo, Gabor, "GABAergic signaling in primary lens epithelial and lentoid cells and its involvement in intracellular Ca2+ modulation" in Cell Calcium, 50, no. 4 (2011):381-392, https://doi.org/10.1016/j.ceca.2011.07.002 . .