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Biotransformation of 4-halophenols to 4-halocatechols using Escherichia coli expressing 4-hydroxyphenylacetate 3-hydroxylase
dc.creator | Coulombel, Lydie | |
dc.creator | Nolan, Louise C. | |
dc.creator | Nikodinović-Runić, Jasmina | |
dc.creator | Doyle, Evelyn M. | |
dc.creator | O'Connor, Kevin | |
dc.date.accessioned | 2022-11-15T14:07:37Z | |
dc.date.available | 2022-11-15T14:07:37Z | |
dc.date.issued | 2011 | |
dc.identifier.issn | 0175-7598 | |
dc.identifier.uri | https://imagine.imgge.bg.ac.rs/handle/123456789/503 | |
dc.description.abstract | Escherichia coli cells, expressing 4-hydroxyphenylacetate 3-hydroxylase, fully transformed 4-halogenated phenols to their equivalent catechols as single products in shaken flasks. 4-Fluorophenol was transformed at a rate 1.6, 1.8, and 3.4-fold higher than the biotransformation of 4-chloro-, 4-bromo-, and 4-iodo- phenol, respectively. A scale-up from shaken flask to a 5 L stirred tank bioreactor was undertaken to develop a bioprocess for the production of 4-substituted halocatechols at higher concentrations and scale. In a stirred tank reactor, the optimized conditions for induction of 4-HPA hydroxylase expression were at 37 C for 3 h. The rate of biotransformation of 4-fluorophenol to 4-fluorocatechol by stirred tank bioreactor grown cells was the same at 1 and 4.8 mM (5.13 mu mol/min/g CDW) once the ratio of biocatalyst (E. coli CDW) to substrate concentration (mM) was maintained at 2:1. At 10.8 mM 4-fluorophenol, the rate of 4-fluorocatechol formation decreased by 4.7-fold. However, the complete transformation of 1.3 g of 4-fluorophenol (10.8 mM) to 4-fluorocatechol was achieved within 7 h in a 1 L reaction volume. Similar to 4-fluorophenol, other 4-substituted halophenols were completely transformed to 4-halocatechols at 2 mM within a 1-2 h period. An increase in 4-halophenol concentration to 4.8 mM resulted in a 2.5-20-fold decrease in biotransformation efficiency depending on the substrate tested. Organic solvent extraction of the 4-halocatechol products followed by column chromatography resulted in the production of purified products with a final yield of between 33% and 38%. | en |
dc.publisher | Springer, New York | |
dc.relation | Enterprise Ireland [PC/2008/131] | |
dc.relation | Environmental Protection Agency [2008-ET-LS-1-S2] | |
dc.relation | Science Foundation Ireland [04/IN3/B581] | |
dc.rights | restrictedAccess | |
dc.source | Applied Microbiology and Biotechnology | |
dc.subject | Scale-up process | en |
dc.subject | Biotransformation | en |
dc.subject | 4-Hydroxyphenylacetate 3-hydroxylase | en |
dc.subject | 4-Halogenated catechols | en |
dc.title | Biotransformation of 4-halophenols to 4-halocatechols using Escherichia coli expressing 4-hydroxyphenylacetate 3-hydroxylase | en |
dc.type | article | |
dc.rights.license | ARR | |
dc.citation.epage | 1875 | |
dc.citation.issue | 6 | |
dc.citation.other | 89(6): 1867-1875 | |
dc.citation.rank | M21 | |
dc.citation.spage | 1867 | |
dc.citation.volume | 89 | |
dc.identifier.doi | 10.1007/s00253-010-2969-5 | |
dc.identifier.pmid | 21057945 | |
dc.identifier.scopus | 2-s2.0-79952575497 | |
dc.identifier.wos | 000288333800021 | |
dc.type.version | publishedVersion |