Uspostavljanje model sistema za transformaciju biljaka I. hawkerii Bull.

Abstract

Jedna od vodećih oblasti savremene biotehnologije je dobijanje biljaka otpornih na patogene pomoću metoda genetičkog inženjeringa. U ovom radu je proučavana genetička transformacija Impatiens hawkerii Bull. pomoću Agrobacterium tumefaciens C58C1pac1, a cilj je dobijanje biljaka koje nose pac1 gen za rezistentnost na virus bronzavosti paradajza i druge biljne RNK viruse. Transformanati I. hawkerii Bull. na selektivnoj podlozi su brzo propadali, a ni jedan klon nije preživeo peti pasaž. Ovo propadanje je posledica velike osetljivosti ove vrste na kanamicin ili neuspešnosti transfomacije.

One of the leading areas of modern biotechnology is to develop plants resistant to pathogens using genetic engineering methods. The genetic transformation of I. hawkerii Bull. with Agrobacterium tumefaciens C58C1pac1 was observe in this study, and the aim was to derive plants carrying the gene for pac1 resistance to tomato spotted wilt virus and other plant RNA viruses. I. hawkerii Bull. transformants on a selective medium rapidly failed, and not a single clone survived the fifth passage. This decline was due to great susceptibility of this type to kanamycin or failure of the transformation.