Comparative analysis of beta-casein proteolysis by PrtP proteinase from Lactobacillus paracasei subsp paracasei BGHN14, PrtR proteinase from Lactobacillus rhamnosus BGT10 and PrtH proteinase from Lactobacillus helveticus BGRA43
Samo za registrovane korisnike
2011
Autori
Lozo, JelenaStrahinić, Ivana
Dalgalarrondo, Michele
Chobert, Jean-Marc
Haertle, Thomas
Topisirović, Ljubiša
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Lactic acid bacteria contain different cell-envelope proteinases responsible for hydrolysis of caseins. Isolates of Lactobacillus paracasei subsp. paracasei BGHN14 and Lactobacillus rhamnosus BGT10 harbour PrtP and PrtR proteinases, respectively, whereas the PrtH proteinase of Lactobacillus helveticus BGRA43 is similar to that of Lb. helveticus CNRZ32. Mass spectrometry analysis of the major peptides isolated by reversed phase-high performance liquid chromatography allowed the identification of 25, 22 and 17 peptides after beta-casein hydrolysis by strains BGHN14, BGRA43 and BGT10, respectively. Regardless of the type of proteinase, beta-casein was hydrolyzed preferentially after hydrophobic residues and glutamines ( Q). PrtP and PrtR proteinases preferentially targeted the C-terminus of beta-casein. In the case of PrtH proteinase, most of the peptides obtained were cleaved from N-terminus of this casein. Nine identical peptides were identified after hydrolysis with PrtP and PrtR, thre...e after proteolysis with PrtR and PrtH, and two after hydrolysis with all three proteinases studied.
Izvor:
International Dairy Journal, 2011, 21, 11, 863-868Izdavač:
- Elsevier Sci Ltd, Oxford
Finansiranje / projekti:
- Izučavanje gena i molekularnih mehanizama u osnovi probiotičke aktivnosti bakterija mlečne kiseline izolovanih sa područja zapadnog Balkana (RS-MESTD-Basic Research (BR or ON)-173019)
- Serbian-French technology cooperation program [23622VL]
- French Ministry of Foreign and European Affairs [21350SD]
DOI: 10.1016/j.idairyj.2011.05.002
ISSN: 0958-6946
WoS: 000293825800004
Scopus: 2-s2.0-80051591771
Institucija/grupa
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Lozo, Jelena AU - Strahinić, Ivana AU - Dalgalarrondo, Michele AU - Chobert, Jean-Marc AU - Haertle, Thomas AU - Topisirović, Ljubiša PY - 2011 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/515 AB - Lactic acid bacteria contain different cell-envelope proteinases responsible for hydrolysis of caseins. Isolates of Lactobacillus paracasei subsp. paracasei BGHN14 and Lactobacillus rhamnosus BGT10 harbour PrtP and PrtR proteinases, respectively, whereas the PrtH proteinase of Lactobacillus helveticus BGRA43 is similar to that of Lb. helveticus CNRZ32. Mass spectrometry analysis of the major peptides isolated by reversed phase-high performance liquid chromatography allowed the identification of 25, 22 and 17 peptides after beta-casein hydrolysis by strains BGHN14, BGRA43 and BGT10, respectively. Regardless of the type of proteinase, beta-casein was hydrolyzed preferentially after hydrophobic residues and glutamines ( Q). PrtP and PrtR proteinases preferentially targeted the C-terminus of beta-casein. In the case of PrtH proteinase, most of the peptides obtained were cleaved from N-terminus of this casein. Nine identical peptides were identified after hydrolysis with PrtP and PrtR, three after proteolysis with PrtR and PrtH, and two after hydrolysis with all three proteinases studied. PB - Elsevier Sci Ltd, Oxford T2 - International Dairy Journal T1 - Comparative analysis of beta-casein proteolysis by PrtP proteinase from Lactobacillus paracasei subsp paracasei BGHN14, PrtR proteinase from Lactobacillus rhamnosus BGT10 and PrtH proteinase from Lactobacillus helveticus BGRA43 EP - 868 IS - 11 SP - 863 VL - 21 DO - 10.1016/j.idairyj.2011.05.002 ER -
@article{ author = "Lozo, Jelena and Strahinić, Ivana and Dalgalarrondo, Michele and Chobert, Jean-Marc and Haertle, Thomas and Topisirović, Ljubiša", year = "2011", abstract = "Lactic acid bacteria contain different cell-envelope proteinases responsible for hydrolysis of caseins. Isolates of Lactobacillus paracasei subsp. paracasei BGHN14 and Lactobacillus rhamnosus BGT10 harbour PrtP and PrtR proteinases, respectively, whereas the PrtH proteinase of Lactobacillus helveticus BGRA43 is similar to that of Lb. helveticus CNRZ32. Mass spectrometry analysis of the major peptides isolated by reversed phase-high performance liquid chromatography allowed the identification of 25, 22 and 17 peptides after beta-casein hydrolysis by strains BGHN14, BGRA43 and BGT10, respectively. Regardless of the type of proteinase, beta-casein was hydrolyzed preferentially after hydrophobic residues and glutamines ( Q). PrtP and PrtR proteinases preferentially targeted the C-terminus of beta-casein. In the case of PrtH proteinase, most of the peptides obtained were cleaved from N-terminus of this casein. Nine identical peptides were identified after hydrolysis with PrtP and PrtR, three after proteolysis with PrtR and PrtH, and two after hydrolysis with all three proteinases studied.", publisher = "Elsevier Sci Ltd, Oxford", journal = "International Dairy Journal", title = "Comparative analysis of beta-casein proteolysis by PrtP proteinase from Lactobacillus paracasei subsp paracasei BGHN14, PrtR proteinase from Lactobacillus rhamnosus BGT10 and PrtH proteinase from Lactobacillus helveticus BGRA43", pages = "868-863", number = "11", volume = "21", doi = "10.1016/j.idairyj.2011.05.002" }
Lozo, J., Strahinić, I., Dalgalarrondo, M., Chobert, J., Haertle, T.,& Topisirović, L.. (2011). Comparative analysis of beta-casein proteolysis by PrtP proteinase from Lactobacillus paracasei subsp paracasei BGHN14, PrtR proteinase from Lactobacillus rhamnosus BGT10 and PrtH proteinase from Lactobacillus helveticus BGRA43. in International Dairy Journal Elsevier Sci Ltd, Oxford., 21(11), 863-868. https://doi.org/10.1016/j.idairyj.2011.05.002
Lozo J, Strahinić I, Dalgalarrondo M, Chobert J, Haertle T, Topisirović L. Comparative analysis of beta-casein proteolysis by PrtP proteinase from Lactobacillus paracasei subsp paracasei BGHN14, PrtR proteinase from Lactobacillus rhamnosus BGT10 and PrtH proteinase from Lactobacillus helveticus BGRA43. in International Dairy Journal. 2011;21(11):863-868. doi:10.1016/j.idairyj.2011.05.002 .
Lozo, Jelena, Strahinić, Ivana, Dalgalarrondo, Michele, Chobert, Jean-Marc, Haertle, Thomas, Topisirović, Ljubiša, "Comparative analysis of beta-casein proteolysis by PrtP proteinase from Lactobacillus paracasei subsp paracasei BGHN14, PrtR proteinase from Lactobacillus rhamnosus BGT10 and PrtH proteinase from Lactobacillus helveticus BGRA43" in International Dairy Journal, 21, no. 11 (2011):863-868, https://doi.org/10.1016/j.idairyj.2011.05.002 . .