Interaction between insulin and estradiol in regulation of cardiac glucose and free fatty acid transporters
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2011
Authors
Tepavcević, S.Koricanac, G.
Zakula, Z.
Milosavljević, T.
Stojiljković, Mojca
Isenović, E.R.
Article (Published version)
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The estrogen binding to specific extranuclear receptors (ER) activates several intracellular pathways that are activated by insulin as well. Moreover, insulin and estradiol (E2) influence cardiac energy substrates, blood glucose and free fatty acids (FFAs), and both hormones exert cardio-beneficial effects. In view of these facts, we suggest that cross-talk between their signaling pathways might have an important role in regulation of cardiac energy substrate transport. Ovariectomized rats were treated with insulin, estradiol (E2), or their combination 20, 30, or 40min before analysis of blood glucose and FFA level, as well as cardiac plasma membranes (PM) and low density microsomes (LDM) content of glucose (GLUT4 and GLUT1) and FFA (CD36) transporters. Insulin, given alone, or in combination with E2, decreased plasma glucose level at all time points, but did not influence FFA level, while E2 treatment itself did not change glucose and FFA concentration. Insulin increased PM GLUT4 and ...GLUT1 content 30 and 40min after treatment and the increases were partially accompanied by decrease in transporter LDM content. E2 increased PM content and decreased LDM content only of GLUT4 at 30min. Insulin generally, and E2 at 20min increased CD36 content in PM fraction. Both hormones decreased CD36 LDM content 20min after administration. Effect of combined treatment mostly did not differ from single hormone treatment, but occasionally, particularly in distribution of GLUT4, combined treatment emphasized single hormone effect, suggesting that insulin and E2 act synergistically in regulation of energy substrate transporters in cardiac tissue.
Keywords:
insulin / heart / glucose transporter type 4 / glucose transporter type 1 / estradiol / CD36 fatty acid transporterSource:
Hormone and Metabolic Research, 2011, 43, 8, 524-530Funding / projects:
- Role of steroid hormones in neuroendocrine adaptation to stress and pathophysiology of metabolic syndrome - molecular mechanisms and clinical implications (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41009)
DOI: 10.1055/s-0031-1280784
ISSN: 0018-5043
PubMed: 21773965
WoS: 000292876100002
Scopus: 2-s2.0-79960698563
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Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Tepavcević, S. AU - Koricanac, G. AU - Zakula, Z. AU - Milosavljević, T. AU - Stojiljković, Mojca AU - Isenović, E.R. PY - 2011 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/523 AB - The estrogen binding to specific extranuclear receptors (ER) activates several intracellular pathways that are activated by insulin as well. Moreover, insulin and estradiol (E2) influence cardiac energy substrates, blood glucose and free fatty acids (FFAs), and both hormones exert cardio-beneficial effects. In view of these facts, we suggest that cross-talk between their signaling pathways might have an important role in regulation of cardiac energy substrate transport. Ovariectomized rats were treated with insulin, estradiol (E2), or their combination 20, 30, or 40min before analysis of blood glucose and FFA level, as well as cardiac plasma membranes (PM) and low density microsomes (LDM) content of glucose (GLUT4 and GLUT1) and FFA (CD36) transporters. Insulin, given alone, or in combination with E2, decreased plasma glucose level at all time points, but did not influence FFA level, while E2 treatment itself did not change glucose and FFA concentration. Insulin increased PM GLUT4 and GLUT1 content 30 and 40min after treatment and the increases were partially accompanied by decrease in transporter LDM content. E2 increased PM content and decreased LDM content only of GLUT4 at 30min. Insulin generally, and E2 at 20min increased CD36 content in PM fraction. Both hormones decreased CD36 LDM content 20min after administration. Effect of combined treatment mostly did not differ from single hormone treatment, but occasionally, particularly in distribution of GLUT4, combined treatment emphasized single hormone effect, suggesting that insulin and E2 act synergistically in regulation of energy substrate transporters in cardiac tissue. T2 - Hormone and Metabolic Research T1 - Interaction between insulin and estradiol in regulation of cardiac glucose and free fatty acid transporters EP - 530 IS - 8 SP - 524 VL - 43 DO - 10.1055/s-0031-1280784 ER -
@article{ author = "Tepavcević, S. and Koricanac, G. and Zakula, Z. and Milosavljević, T. and Stojiljković, Mojca and Isenović, E.R.", year = "2011", abstract = "The estrogen binding to specific extranuclear receptors (ER) activates several intracellular pathways that are activated by insulin as well. Moreover, insulin and estradiol (E2) influence cardiac energy substrates, blood glucose and free fatty acids (FFAs), and both hormones exert cardio-beneficial effects. In view of these facts, we suggest that cross-talk between their signaling pathways might have an important role in regulation of cardiac energy substrate transport. Ovariectomized rats were treated with insulin, estradiol (E2), or their combination 20, 30, or 40min before analysis of blood glucose and FFA level, as well as cardiac plasma membranes (PM) and low density microsomes (LDM) content of glucose (GLUT4 and GLUT1) and FFA (CD36) transporters. Insulin, given alone, or in combination with E2, decreased plasma glucose level at all time points, but did not influence FFA level, while E2 treatment itself did not change glucose and FFA concentration. Insulin increased PM GLUT4 and GLUT1 content 30 and 40min after treatment and the increases were partially accompanied by decrease in transporter LDM content. E2 increased PM content and decreased LDM content only of GLUT4 at 30min. Insulin generally, and E2 at 20min increased CD36 content in PM fraction. Both hormones decreased CD36 LDM content 20min after administration. Effect of combined treatment mostly did not differ from single hormone treatment, but occasionally, particularly in distribution of GLUT4, combined treatment emphasized single hormone effect, suggesting that insulin and E2 act synergistically in regulation of energy substrate transporters in cardiac tissue.", journal = "Hormone and Metabolic Research", title = "Interaction between insulin and estradiol in regulation of cardiac glucose and free fatty acid transporters", pages = "530-524", number = "8", volume = "43", doi = "10.1055/s-0031-1280784" }
Tepavcević, S., Koricanac, G., Zakula, Z., Milosavljević, T., Stojiljković, M.,& Isenović, E.R.. (2011). Interaction between insulin and estradiol in regulation of cardiac glucose and free fatty acid transporters. in Hormone and Metabolic Research, 43(8), 524-530. https://doi.org/10.1055/s-0031-1280784
Tepavcević S, Koricanac G, Zakula Z, Milosavljević T, Stojiljković M, Isenović E. Interaction between insulin and estradiol in regulation of cardiac glucose and free fatty acid transporters. in Hormone and Metabolic Research. 2011;43(8):524-530. doi:10.1055/s-0031-1280784 .
Tepavcević, S., Koricanac, G., Zakula, Z., Milosavljević, T., Stojiljković, Mojca, Isenović, E.R., "Interaction between insulin and estradiol in regulation of cardiac glucose and free fatty acid transporters" in Hormone and Metabolic Research, 43, no. 8 (2011):524-530, https://doi.org/10.1055/s-0031-1280784 . .