Interference between insulin and estradiol signaling pathways in the regulation of cardiac eNOS and Na+/K+-ATPase
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2011
Authors
Koricanac, G.Tepavcević, S.
Zakula, Z.
Milosavljević, T.
Stojiljković, Mojca
Isenović, E.R.
Article (Published version)
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Insulin and estradiol share some of signaling pathways and regulate same target molecules exerting mostly beneficial cardiac effects. In order to study their cardiac interaction, ovariectomized female rats were treated with hormones, separately or simultaneously (20, 30 or 40 min before analysis), and the phosphorylations of protein kinase B (Akt), extracellular signal-regulated kinases 1 and 2 (ERK 1/2), endothelial nitric oxide synthase (eNOS) were analyzed, as well as the plasma membrane content of α2 subunit of Na +/K+-ATPase. Insulin, particularly, and estradiol stimulate Ser473 Akt phosphorylation. The combined treatment was stimulatory, but less than insulin alone was. The general increase of Thr 308 Akt phosphorylation by insulin was stronger than at Ser 473 and reduced in the presence of estradiol, which also stimulated this phosphorylation given alone. The estradiol induction of ERK 1/2 phosphorylation was inverted to the decrease by the combined treatment, while insulin had ...no effect. Only insulin increased the plasma membrane content of α2. Estradiol did increase the phosphorylation of eNOS, whereas the insulin effect was controversial. The effect of the combined treatment on target molecules was generally opposite to single hormone treatment. In summary, both hormones exerted an effect on Akt phosphorylation, but only estradiol stimulated ERK 1/2 phosphorylation. The α2 plasma membrane content was increased only by insulin, while estradiol increased eNOS phosphorylation more consistently. Finally, if these hormones were administered together, it seems that they disturb each other in having a full effect on cardiac Akt, ERK 1/2, and downstream effectors, eNOS and Na+/K+-ATPase.
Keywords:
Sodium / Protein kinase B / Potassium ATPase / Nitric oxide synthase type III / Insulin / Extracellular signal-regulated MAP kinase / EstradiolSource:
European Journal of Pharmacology, 2011, 655, 1-3, 23-30Funding / projects:
- Role of steroid hormones in neuroendocrine adaptation to stress and pathophysiology of metabolic syndrome - molecular mechanisms and clinical implications (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41009)
- Hormonal regulation of expression and activity of the nitric oxide synthase and sodium-potassium pump in experimental models of insulin resistance, diabetes and cardiovascular disorders (RS-MESTD-Basic Research (BR or ON)-173033)
DOI: 10.1016/j.ejphar.2011.01.016
ISSN: 0014-2999
PubMed: 21272573
WoS: 000288841000004
Scopus: 2-s2.0-79952535037
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Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Koricanac, G. AU - Tepavcević, S. AU - Zakula, Z. AU - Milosavljević, T. AU - Stojiljković, Mojca AU - Isenović, E.R. PY - 2011 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/524 AB - Insulin and estradiol share some of signaling pathways and regulate same target molecules exerting mostly beneficial cardiac effects. In order to study their cardiac interaction, ovariectomized female rats were treated with hormones, separately or simultaneously (20, 30 or 40 min before analysis), and the phosphorylations of protein kinase B (Akt), extracellular signal-regulated kinases 1 and 2 (ERK 1/2), endothelial nitric oxide synthase (eNOS) were analyzed, as well as the plasma membrane content of α2 subunit of Na +/K+-ATPase. Insulin, particularly, and estradiol stimulate Ser473 Akt phosphorylation. The combined treatment was stimulatory, but less than insulin alone was. The general increase of Thr 308 Akt phosphorylation by insulin was stronger than at Ser 473 and reduced in the presence of estradiol, which also stimulated this phosphorylation given alone. The estradiol induction of ERK 1/2 phosphorylation was inverted to the decrease by the combined treatment, while insulin had no effect. Only insulin increased the plasma membrane content of α2. Estradiol did increase the phosphorylation of eNOS, whereas the insulin effect was controversial. The effect of the combined treatment on target molecules was generally opposite to single hormone treatment. In summary, both hormones exerted an effect on Akt phosphorylation, but only estradiol stimulated ERK 1/2 phosphorylation. The α2 plasma membrane content was increased only by insulin, while estradiol increased eNOS phosphorylation more consistently. Finally, if these hormones were administered together, it seems that they disturb each other in having a full effect on cardiac Akt, ERK 1/2, and downstream effectors, eNOS and Na+/K+-ATPase. T2 - European Journal of Pharmacology T1 - Interference between insulin and estradiol signaling pathways in the regulation of cardiac eNOS and Na+/K+-ATPase EP - 30 IS - 1-3 SP - 23 VL - 655 DO - 10.1016/j.ejphar.2011.01.016 ER -
@article{ author = "Koricanac, G. and Tepavcević, S. and Zakula, Z. and Milosavljević, T. and Stojiljković, Mojca and Isenović, E.R.", year = "2011", abstract = "Insulin and estradiol share some of signaling pathways and regulate same target molecules exerting mostly beneficial cardiac effects. In order to study their cardiac interaction, ovariectomized female rats were treated with hormones, separately or simultaneously (20, 30 or 40 min before analysis), and the phosphorylations of protein kinase B (Akt), extracellular signal-regulated kinases 1 and 2 (ERK 1/2), endothelial nitric oxide synthase (eNOS) were analyzed, as well as the plasma membrane content of α2 subunit of Na +/K+-ATPase. Insulin, particularly, and estradiol stimulate Ser473 Akt phosphorylation. The combined treatment was stimulatory, but less than insulin alone was. The general increase of Thr 308 Akt phosphorylation by insulin was stronger than at Ser 473 and reduced in the presence of estradiol, which also stimulated this phosphorylation given alone. The estradiol induction of ERK 1/2 phosphorylation was inverted to the decrease by the combined treatment, while insulin had no effect. Only insulin increased the plasma membrane content of α2. Estradiol did increase the phosphorylation of eNOS, whereas the insulin effect was controversial. The effect of the combined treatment on target molecules was generally opposite to single hormone treatment. In summary, both hormones exerted an effect on Akt phosphorylation, but only estradiol stimulated ERK 1/2 phosphorylation. The α2 plasma membrane content was increased only by insulin, while estradiol increased eNOS phosphorylation more consistently. Finally, if these hormones were administered together, it seems that they disturb each other in having a full effect on cardiac Akt, ERK 1/2, and downstream effectors, eNOS and Na+/K+-ATPase.", journal = "European Journal of Pharmacology", title = "Interference between insulin and estradiol signaling pathways in the regulation of cardiac eNOS and Na+/K+-ATPase", pages = "30-23", number = "1-3", volume = "655", doi = "10.1016/j.ejphar.2011.01.016" }
Koricanac, G., Tepavcević, S., Zakula, Z., Milosavljević, T., Stojiljković, M.,& Isenović, E.R.. (2011). Interference between insulin and estradiol signaling pathways in the regulation of cardiac eNOS and Na+/K+-ATPase. in European Journal of Pharmacology, 655(1-3), 23-30. https://doi.org/10.1016/j.ejphar.2011.01.016
Koricanac G, Tepavcević S, Zakula Z, Milosavljević T, Stojiljković M, Isenović E. Interference between insulin and estradiol signaling pathways in the regulation of cardiac eNOS and Na+/K+-ATPase. in European Journal of Pharmacology. 2011;655(1-3):23-30. doi:10.1016/j.ejphar.2011.01.016 .
Koricanac, G., Tepavcević, S., Zakula, Z., Milosavljević, T., Stojiljković, Mojca, Isenović, E.R., "Interference between insulin and estradiol signaling pathways in the regulation of cardiac eNOS and Na+/K+-ATPase" in European Journal of Pharmacology, 655, no. 1-3 (2011):23-30, https://doi.org/10.1016/j.ejphar.2011.01.016 . .