Selection of indigenous fluorescent pseudomonad isolates from maize rhizospheric soil in Vojvodina as possible PGPR
Apstrakt
Among 268 bacterial isolates obtained from maize rhizospheric soil from Novi Sad, Vojvodina. Serbia. 59 were fluorescent pseudomonads. According to intrinsic antibiotic resistance (JAR) and heavy metal tolerance (HMT) patterns, the six representative isolates were selected and their diversity was assessed. Lytic enzyme activity (protease, chitinase, lipase, phospolipase, cellulase, gellatinase, pectinase, urease), plant-growth promoting treats (P-solubilization, siderophores, HCN and IAA production) and plant pathogenicity estimation (on lilac leaf apple and bean pods) resulted in selection of three isolates (PS2, P54, PS6) with the outlook for agriculture application. Antifungal activity was estimated on dual culture with eight phytopathogenic fungi (Curvularia lunata, Fusarium semitectum, Fusarium equiseti from Salvia officinalis L., F.equiseti from Matricaria chamomilla L.. Myrothecium verrucaria, Verticillium sp., Diaporte eres complex and Sclerotinia sclerotiorum) isolated from me...dicinal plants in Serbia. Isolate PS2 showed hyphal deformation of all investigated fungi and effective inhibition of mycelial growth of 7 out of 8 phytopatogenic fungi, partly due to production of chitinases, siderpohores and lytic enzymes. Abundant production of IAA (14 to 37 mM) and siderophores, phosphate solubilization and especially fungal growth inhibition make it suitable for further investigation, field trials and possible application in maize cultivation as biocontrol agent.
Ključne reči:
Pseudomonas / plant-growth promoting rhizobacteria / biocontrol agents / antifungal activityIzvor:
Romanian Biotechnological Letters, 2011, 16, 5, 6580-6590Finansiranje / projekti:
- Novi autohtoni izolati bakterija Lysobacter i Pseudomonas kao važan izvor metabolita korisnih za biotehnologiju, stimulaciju rasta biljaka i kontrolu bolesti bilja: od izolata do preparata (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46007)
Institucija/grupa
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Đurić, S. AU - Pavić, Aleksandar AU - Jarak, M. AU - Pavlović, S. AU - Starović, M. AU - Pivić, R. AU - Josić, D. PY - 2011 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/527 AB - Among 268 bacterial isolates obtained from maize rhizospheric soil from Novi Sad, Vojvodina. Serbia. 59 were fluorescent pseudomonads. According to intrinsic antibiotic resistance (JAR) and heavy metal tolerance (HMT) patterns, the six representative isolates were selected and their diversity was assessed. Lytic enzyme activity (protease, chitinase, lipase, phospolipase, cellulase, gellatinase, pectinase, urease), plant-growth promoting treats (P-solubilization, siderophores, HCN and IAA production) and plant pathogenicity estimation (on lilac leaf apple and bean pods) resulted in selection of three isolates (PS2, P54, PS6) with the outlook for agriculture application. Antifungal activity was estimated on dual culture with eight phytopathogenic fungi (Curvularia lunata, Fusarium semitectum, Fusarium equiseti from Salvia officinalis L., F.equiseti from Matricaria chamomilla L.. Myrothecium verrucaria, Verticillium sp., Diaporte eres complex and Sclerotinia sclerotiorum) isolated from medicinal plants in Serbia. Isolate PS2 showed hyphal deformation of all investigated fungi and effective inhibition of mycelial growth of 7 out of 8 phytopatogenic fungi, partly due to production of chitinases, siderpohores and lytic enzymes. Abundant production of IAA (14 to 37 mM) and siderophores, phosphate solubilization and especially fungal growth inhibition make it suitable for further investigation, field trials and possible application in maize cultivation as biocontrol agent. T2 - Romanian Biotechnological Letters T1 - Selection of indigenous fluorescent pseudomonad isolates from maize rhizospheric soil in Vojvodina as possible PGPR EP - 6590 IS - 5 SP - 6580 VL - 16 UR - https://hdl.handle.net/21.15107/rcub_imagine_527 ER -
@article{ author = "Đurić, S. and Pavić, Aleksandar and Jarak, M. and Pavlović, S. and Starović, M. and Pivić, R. and Josić, D.", year = "2011", abstract = "Among 268 bacterial isolates obtained from maize rhizospheric soil from Novi Sad, Vojvodina. Serbia. 59 were fluorescent pseudomonads. According to intrinsic antibiotic resistance (JAR) and heavy metal tolerance (HMT) patterns, the six representative isolates were selected and their diversity was assessed. Lytic enzyme activity (protease, chitinase, lipase, phospolipase, cellulase, gellatinase, pectinase, urease), plant-growth promoting treats (P-solubilization, siderophores, HCN and IAA production) and plant pathogenicity estimation (on lilac leaf apple and bean pods) resulted in selection of three isolates (PS2, P54, PS6) with the outlook for agriculture application. Antifungal activity was estimated on dual culture with eight phytopathogenic fungi (Curvularia lunata, Fusarium semitectum, Fusarium equiseti from Salvia officinalis L., F.equiseti from Matricaria chamomilla L.. Myrothecium verrucaria, Verticillium sp., Diaporte eres complex and Sclerotinia sclerotiorum) isolated from medicinal plants in Serbia. Isolate PS2 showed hyphal deformation of all investigated fungi and effective inhibition of mycelial growth of 7 out of 8 phytopatogenic fungi, partly due to production of chitinases, siderpohores and lytic enzymes. Abundant production of IAA (14 to 37 mM) and siderophores, phosphate solubilization and especially fungal growth inhibition make it suitable for further investigation, field trials and possible application in maize cultivation as biocontrol agent.", journal = "Romanian Biotechnological Letters", title = "Selection of indigenous fluorescent pseudomonad isolates from maize rhizospheric soil in Vojvodina as possible PGPR", pages = "6590-6580", number = "5", volume = "16", url = "https://hdl.handle.net/21.15107/rcub_imagine_527" }
Đurić, S., Pavić, A., Jarak, M., Pavlović, S., Starović, M., Pivić, R.,& Josić, D.. (2011). Selection of indigenous fluorescent pseudomonad isolates from maize rhizospheric soil in Vojvodina as possible PGPR. in Romanian Biotechnological Letters, 16(5), 6580-6590. https://hdl.handle.net/21.15107/rcub_imagine_527
Đurić S, Pavić A, Jarak M, Pavlović S, Starović M, Pivić R, Josić D. Selection of indigenous fluorescent pseudomonad isolates from maize rhizospheric soil in Vojvodina as possible PGPR. in Romanian Biotechnological Letters. 2011;16(5):6580-6590. https://hdl.handle.net/21.15107/rcub_imagine_527 .
Đurić, S., Pavić, Aleksandar, Jarak, M., Pavlović, S., Starović, M., Pivić, R., Josić, D., "Selection of indigenous fluorescent pseudomonad isolates from maize rhizospheric soil in Vojvodina as possible PGPR" in Romanian Biotechnological Letters, 16, no. 5 (2011):6580-6590, https://hdl.handle.net/21.15107/rcub_imagine_527 .