Dissipation of Proton Motive Force is not Sufficient to Induce the Phage Shock Protein Response in Escherichia coli
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2011
Authors
Engl, Christoph![](/themes/Mirageimagine/images/orcid.png)
Ter Beek, Alex
Bekker, Martijn
de Mattos, Joost Teixeira
Jovanović, Goran
![](/themes/Mirageimagine/images/orcid.png)
Buck, Martin
Article (Published version)
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Phage shock proteins (Psp) and their homologues are found in species from the three domains of life: Bacteria, Archaea and Eukarya (e.g. higher plants). In enterobacteria, the Psp response helps to maintain the proton motive force (PMF) of the cell when the inner membrane integrity is impaired. The presumed ability of ArcB to sense redox changes in the cellular quinone pool and the strong decrease of psp induction in Delta ubiG or Delta arcAB backgrounds suggest a link between the Psp response and the quinone pool. The authors now provide evidence indicating that the physiological signal for inducing psp by secretin-induced stress is neither the quinone redox state nor a drop in PMF. Neither the loss of the H+-gradient nor the dissipation of the electrical potential alone is sufficient to induce the Psp response. A set of electron transport mutants differing in their redox states due to the lack of a NADH dehydrogenase and a quinol oxidase, but retaining a normal PMF displayed low leve...ls of psp induction inversely related to oxidised ubiquinone levels under microaerobic growth and independent of PMF. In contrast, cells displaying higher secretin induced psp expression showed increased levels of ubiquinone. Taken together, this study suggests that not a single but likely multiple signals are needed to be integrated to induce the Psp response.
Source:
Current Microbiology, 2011, 62, 5, 1374-1385Publisher:
- Springer, New York
Funding / projects:
- The Wellcome Trust
- IC Postgraduate Studentship
- NWO-SysMO [826.06.002]
DOI: 10.1007/s00284-011-9869-5
ISSN: 0343-8651
PubMed: 21259006
WoS: 000289110000005
Scopus: 2-s2.0-79954426587
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Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Engl, Christoph AU - Ter Beek, Alex AU - Bekker, Martijn AU - de Mattos, Joost Teixeira AU - Jovanović, Goran AU - Buck, Martin PY - 2011 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/530 AB - Phage shock proteins (Psp) and their homologues are found in species from the three domains of life: Bacteria, Archaea and Eukarya (e.g. higher plants). In enterobacteria, the Psp response helps to maintain the proton motive force (PMF) of the cell when the inner membrane integrity is impaired. The presumed ability of ArcB to sense redox changes in the cellular quinone pool and the strong decrease of psp induction in Delta ubiG or Delta arcAB backgrounds suggest a link between the Psp response and the quinone pool. The authors now provide evidence indicating that the physiological signal for inducing psp by secretin-induced stress is neither the quinone redox state nor a drop in PMF. Neither the loss of the H+-gradient nor the dissipation of the electrical potential alone is sufficient to induce the Psp response. A set of electron transport mutants differing in their redox states due to the lack of a NADH dehydrogenase and a quinol oxidase, but retaining a normal PMF displayed low levels of psp induction inversely related to oxidised ubiquinone levels under microaerobic growth and independent of PMF. In contrast, cells displaying higher secretin induced psp expression showed increased levels of ubiquinone. Taken together, this study suggests that not a single but likely multiple signals are needed to be integrated to induce the Psp response. PB - Springer, New York T2 - Current Microbiology T1 - Dissipation of Proton Motive Force is not Sufficient to Induce the Phage Shock Protein Response in Escherichia coli EP - 1385 IS - 5 SP - 1374 VL - 62 DO - 10.1007/s00284-011-9869-5 ER -
@article{ author = "Engl, Christoph and Ter Beek, Alex and Bekker, Martijn and de Mattos, Joost Teixeira and Jovanović, Goran and Buck, Martin", year = "2011", abstract = "Phage shock proteins (Psp) and their homologues are found in species from the three domains of life: Bacteria, Archaea and Eukarya (e.g. higher plants). In enterobacteria, the Psp response helps to maintain the proton motive force (PMF) of the cell when the inner membrane integrity is impaired. The presumed ability of ArcB to sense redox changes in the cellular quinone pool and the strong decrease of psp induction in Delta ubiG or Delta arcAB backgrounds suggest a link between the Psp response and the quinone pool. The authors now provide evidence indicating that the physiological signal for inducing psp by secretin-induced stress is neither the quinone redox state nor a drop in PMF. Neither the loss of the H+-gradient nor the dissipation of the electrical potential alone is sufficient to induce the Psp response. A set of electron transport mutants differing in their redox states due to the lack of a NADH dehydrogenase and a quinol oxidase, but retaining a normal PMF displayed low levels of psp induction inversely related to oxidised ubiquinone levels under microaerobic growth and independent of PMF. In contrast, cells displaying higher secretin induced psp expression showed increased levels of ubiquinone. Taken together, this study suggests that not a single but likely multiple signals are needed to be integrated to induce the Psp response.", publisher = "Springer, New York", journal = "Current Microbiology", title = "Dissipation of Proton Motive Force is not Sufficient to Induce the Phage Shock Protein Response in Escherichia coli", pages = "1385-1374", number = "5", volume = "62", doi = "10.1007/s00284-011-9869-5" }
Engl, C., Ter Beek, A., Bekker, M., de Mattos, J. T., Jovanović, G.,& Buck, M.. (2011). Dissipation of Proton Motive Force is not Sufficient to Induce the Phage Shock Protein Response in Escherichia coli. in Current Microbiology Springer, New York., 62(5), 1374-1385. https://doi.org/10.1007/s00284-011-9869-5
Engl C, Ter Beek A, Bekker M, de Mattos JT, Jovanović G, Buck M. Dissipation of Proton Motive Force is not Sufficient to Induce the Phage Shock Protein Response in Escherichia coli. in Current Microbiology. 2011;62(5):1374-1385. doi:10.1007/s00284-011-9869-5 .
Engl, Christoph, Ter Beek, Alex, Bekker, Martijn, de Mattos, Joost Teixeira, Jovanović, Goran, Buck, Martin, "Dissipation of Proton Motive Force is not Sufficient to Induce the Phage Shock Protein Response in Escherichia coli" in Current Microbiology, 62, no. 5 (2011):1374-1385, https://doi.org/10.1007/s00284-011-9869-5 . .