Molecular typing and antimicrobial resistance of Salmonella Enteritidis isolated from poultry, food, and humans in Serbia
Само за регистроване кориснике
2011
Аутори
Kozoderović, G.Velhner, Maja
Jelesić, Z.
Stojanov, I.
Petrović, T.
Stojanović, D.
Golić, Nataša
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Molecular typing and resistotyping coupled with gyrA single nucleotide polymorphism (SNP) of 60 Salmonella Enteritidis (SE) isolates originated from poultry, food, and humans in Serbia is described. Molecular fingerprinting was performed by randomly amplified polymorphic DNA (RAPD) using four primers, and the diversity index (D) was 0.688. In combination with resistotyping and gyrA SNP, D increased to 0.828. A total of 23 genetic groups were obtained. When four RAPD primers were combined, epidemic isolates from a fast-food restaurant outbreak were clustered in a distinctive genetic group. Among 60 SE strains, three had multiple resistances to three or more antibiotics. Nine strains were resistant to nalidixic acid (NAL; a non-fluorinated quinolone). The mutations in quinolone resistance-determining region (QRDR) found in NAL-resistant strains were attributed to Asp(87) - gt Asn in six strains, Asp(87) - gt Gly in one strain, and Ser(83) - gt Phe in one strain. One NAL-resistant stra...in had no mutations in QRDR, suggesting another mechanism of resistance.
Извор:
Folia Microbiologica, 2011, 56, 1, 66-71Издавач:
- Springer, Dordrecht
Финансирање / пројекти:
- Биотехнолошки поступци у етиолошкој дијагностици вирусних и бактеријских инфекција, контаминаната хране и биолошких производа у ветеринарској медицини (RS-MESTD-MPN2006-2010-20115)
DOI: 10.1007/s12223-011-0003-7
ISSN: 0015-5632
PubMed: 21394475
WoS: 000290451500011
Scopus: 2-s2.0-79955843616
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Kozoderović, G. AU - Velhner, Maja AU - Jelesić, Z. AU - Stojanov, I. AU - Petrović, T. AU - Stojanović, D. AU - Golić, Nataša PY - 2011 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/532 AB - Molecular typing and resistotyping coupled with gyrA single nucleotide polymorphism (SNP) of 60 Salmonella Enteritidis (SE) isolates originated from poultry, food, and humans in Serbia is described. Molecular fingerprinting was performed by randomly amplified polymorphic DNA (RAPD) using four primers, and the diversity index (D) was 0.688. In combination with resistotyping and gyrA SNP, D increased to 0.828. A total of 23 genetic groups were obtained. When four RAPD primers were combined, epidemic isolates from a fast-food restaurant outbreak were clustered in a distinctive genetic group. Among 60 SE strains, three had multiple resistances to three or more antibiotics. Nine strains were resistant to nalidixic acid (NAL; a non-fluorinated quinolone). The mutations in quinolone resistance-determining region (QRDR) found in NAL-resistant strains were attributed to Asp(87) - gt Asn in six strains, Asp(87) - gt Gly in one strain, and Ser(83) - gt Phe in one strain. One NAL-resistant strain had no mutations in QRDR, suggesting another mechanism of resistance. PB - Springer, Dordrecht T2 - Folia Microbiologica T1 - Molecular typing and antimicrobial resistance of Salmonella Enteritidis isolated from poultry, food, and humans in Serbia EP - 71 IS - 1 SP - 66 VL - 56 DO - 10.1007/s12223-011-0003-7 ER -
@article{ author = "Kozoderović, G. and Velhner, Maja and Jelesić, Z. and Stojanov, I. and Petrović, T. and Stojanović, D. and Golić, Nataša", year = "2011", abstract = "Molecular typing and resistotyping coupled with gyrA single nucleotide polymorphism (SNP) of 60 Salmonella Enteritidis (SE) isolates originated from poultry, food, and humans in Serbia is described. Molecular fingerprinting was performed by randomly amplified polymorphic DNA (RAPD) using four primers, and the diversity index (D) was 0.688. In combination with resistotyping and gyrA SNP, D increased to 0.828. A total of 23 genetic groups were obtained. When four RAPD primers were combined, epidemic isolates from a fast-food restaurant outbreak were clustered in a distinctive genetic group. Among 60 SE strains, three had multiple resistances to three or more antibiotics. Nine strains were resistant to nalidixic acid (NAL; a non-fluorinated quinolone). The mutations in quinolone resistance-determining region (QRDR) found in NAL-resistant strains were attributed to Asp(87) - gt Asn in six strains, Asp(87) - gt Gly in one strain, and Ser(83) - gt Phe in one strain. One NAL-resistant strain had no mutations in QRDR, suggesting another mechanism of resistance.", publisher = "Springer, Dordrecht", journal = "Folia Microbiologica", title = "Molecular typing and antimicrobial resistance of Salmonella Enteritidis isolated from poultry, food, and humans in Serbia", pages = "71-66", number = "1", volume = "56", doi = "10.1007/s12223-011-0003-7" }
Kozoderović, G., Velhner, M., Jelesić, Z., Stojanov, I., Petrović, T., Stojanović, D.,& Golić, N.. (2011). Molecular typing and antimicrobial resistance of Salmonella Enteritidis isolated from poultry, food, and humans in Serbia. in Folia Microbiologica Springer, Dordrecht., 56(1), 66-71. https://doi.org/10.1007/s12223-011-0003-7
Kozoderović G, Velhner M, Jelesić Z, Stojanov I, Petrović T, Stojanović D, Golić N. Molecular typing and antimicrobial resistance of Salmonella Enteritidis isolated from poultry, food, and humans in Serbia. in Folia Microbiologica. 2011;56(1):66-71. doi:10.1007/s12223-011-0003-7 .
Kozoderović, G., Velhner, Maja, Jelesić, Z., Stojanov, I., Petrović, T., Stojanović, D., Golić, Nataša, "Molecular typing and antimicrobial resistance of Salmonella Enteritidis isolated from poultry, food, and humans in Serbia" in Folia Microbiologica, 56, no. 1 (2011):66-71, https://doi.org/10.1007/s12223-011-0003-7 . .