Cell-type dependent response of melanoma cells to aloe emodin
Само за регистроване кориснике
2012
Аутори
Radović, J.Maksimović-Ivanić, Danijela
Timotijević, Gordana
Popadić, S.
Ramić, Z.
Trajković, V.
Miljković, D.
Stošić-Grujičić, Stanislava
Mijatović, S.
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Intrinsic characteristics of melanoma cells such as expression of inducible nitric oxide synthase (iNOS), redox status, and activity of signaling pathways involved in proliferation, differentiation and cell death define the response of the cells to the diverse treatments. In this context we compared the effectiveness of herbal antaquinone aloe emodin (AE) against mouse B16 melanoma and human A375, different in initial activity of ERK1/2, constitutive iNOS expression and basal level of reactive oxygen species (ROS). Both cell lines are sensitive to AE treatment. However, while the agent induces differentiation of B16 cells toward melanocytes, in A375 cells promoted massive apoptosis. Differentiation of B16 cells, characterized by enhanced melanin production and tyrosinase activity, was mediated by H2O2 production synchronized with rapid p53 accumulation and enhanced expression of cyclins D1 and D3. Caspase mediated apoptosis triggered in A375 cells was accompanied with Bcl-2 but not iNO...S down-regulation. In addition, opposite regulation of Akt-ERK1/2 axis in AE treated B16 and A375 cells correlated with different outcome of the treatment. However, AE in a dose-dependent manner rescued both B16 and A375 cells from doxorubicin- or paclitaxel-induced killing. These data indicate that caution is warranted when AE is administrated to the patients with conventional chemotherapy.
Кључне речи:
Melanoma / ERK1/2 / Differentiation / Apoptosis / Aloe emodin / AktИзвор:
Food and Chemical Toxicology, 2012, 50, 9, 3181-3189Издавач:
- Pergamon-Elsevier Science Ltd, Oxford
Финансирање / пројекти:
- Молекуларни механизми физиолошке и фармаколошке контроле инфламације и канцера (RS-MESTD-Basic Research (BR or ON)-173013)
DOI: 10.1016/j.fct.2012.05.047
ISSN: 0278-6915
PubMed: 22683487
WoS: 000308624500031
Scopus: 2-s2.0-84864142459
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Radović, J. AU - Maksimović-Ivanić, Danijela AU - Timotijević, Gordana AU - Popadić, S. AU - Ramić, Z. AU - Trajković, V. AU - Miljković, D. AU - Stošić-Grujičić, Stanislava AU - Mijatović, S. PY - 2012 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/550 AB - Intrinsic characteristics of melanoma cells such as expression of inducible nitric oxide synthase (iNOS), redox status, and activity of signaling pathways involved in proliferation, differentiation and cell death define the response of the cells to the diverse treatments. In this context we compared the effectiveness of herbal antaquinone aloe emodin (AE) against mouse B16 melanoma and human A375, different in initial activity of ERK1/2, constitutive iNOS expression and basal level of reactive oxygen species (ROS). Both cell lines are sensitive to AE treatment. However, while the agent induces differentiation of B16 cells toward melanocytes, in A375 cells promoted massive apoptosis. Differentiation of B16 cells, characterized by enhanced melanin production and tyrosinase activity, was mediated by H2O2 production synchronized with rapid p53 accumulation and enhanced expression of cyclins D1 and D3. Caspase mediated apoptosis triggered in A375 cells was accompanied with Bcl-2 but not iNOS down-regulation. In addition, opposite regulation of Akt-ERK1/2 axis in AE treated B16 and A375 cells correlated with different outcome of the treatment. However, AE in a dose-dependent manner rescued both B16 and A375 cells from doxorubicin- or paclitaxel-induced killing. These data indicate that caution is warranted when AE is administrated to the patients with conventional chemotherapy. PB - Pergamon-Elsevier Science Ltd, Oxford T2 - Food and Chemical Toxicology T1 - Cell-type dependent response of melanoma cells to aloe emodin EP - 3189 IS - 9 SP - 3181 VL - 50 DO - 10.1016/j.fct.2012.05.047 ER -
@article{ author = "Radović, J. and Maksimović-Ivanić, Danijela and Timotijević, Gordana and Popadić, S. and Ramić, Z. and Trajković, V. and Miljković, D. and Stošić-Grujičić, Stanislava and Mijatović, S.", year = "2012", abstract = "Intrinsic characteristics of melanoma cells such as expression of inducible nitric oxide synthase (iNOS), redox status, and activity of signaling pathways involved in proliferation, differentiation and cell death define the response of the cells to the diverse treatments. In this context we compared the effectiveness of herbal antaquinone aloe emodin (AE) against mouse B16 melanoma and human A375, different in initial activity of ERK1/2, constitutive iNOS expression and basal level of reactive oxygen species (ROS). Both cell lines are sensitive to AE treatment. However, while the agent induces differentiation of B16 cells toward melanocytes, in A375 cells promoted massive apoptosis. Differentiation of B16 cells, characterized by enhanced melanin production and tyrosinase activity, was mediated by H2O2 production synchronized with rapid p53 accumulation and enhanced expression of cyclins D1 and D3. Caspase mediated apoptosis triggered in A375 cells was accompanied with Bcl-2 but not iNOS down-regulation. In addition, opposite regulation of Akt-ERK1/2 axis in AE treated B16 and A375 cells correlated with different outcome of the treatment. However, AE in a dose-dependent manner rescued both B16 and A375 cells from doxorubicin- or paclitaxel-induced killing. These data indicate that caution is warranted when AE is administrated to the patients with conventional chemotherapy.", publisher = "Pergamon-Elsevier Science Ltd, Oxford", journal = "Food and Chemical Toxicology", title = "Cell-type dependent response of melanoma cells to aloe emodin", pages = "3189-3181", number = "9", volume = "50", doi = "10.1016/j.fct.2012.05.047" }
Radović, J., Maksimović-Ivanić, D., Timotijević, G., Popadić, S., Ramić, Z., Trajković, V., Miljković, D., Stošić-Grujičić, S.,& Mijatović, S.. (2012). Cell-type dependent response of melanoma cells to aloe emodin. in Food and Chemical Toxicology Pergamon-Elsevier Science Ltd, Oxford., 50(9), 3181-3189. https://doi.org/10.1016/j.fct.2012.05.047
Radović J, Maksimović-Ivanić D, Timotijević G, Popadić S, Ramić Z, Trajković V, Miljković D, Stošić-Grujičić S, Mijatović S. Cell-type dependent response of melanoma cells to aloe emodin. in Food and Chemical Toxicology. 2012;50(9):3181-3189. doi:10.1016/j.fct.2012.05.047 .
Radović, J., Maksimović-Ivanić, Danijela, Timotijević, Gordana, Popadić, S., Ramić, Z., Trajković, V., Miljković, D., Stošić-Grujičić, Stanislava, Mijatović, S., "Cell-type dependent response of melanoma cells to aloe emodin" in Food and Chemical Toxicology, 50, no. 9 (2012):3181-3189, https://doi.org/10.1016/j.fct.2012.05.047 . .