Immunomodulatory Properties of Nanoparticles Obtained by Ultrasonic Spray Pirolysis from Gold Scrap
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2012
Authors
Đokić, JelenaRudolf, Rebeka
Tomić, Sergej
Stopić, Srecko
Friedrich, Bernd
Budić, Bojan
Anzel, Ivan
Čolić, Miodrag
Article (Published version)
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We prepared 5 different fractions of nanoparticles from the gold scrap, by using a new technology, Ultrasonic Spray Pirolysis (USP). The aim of this study was to characterize the microstructure and cytotoxicity of the nanoparticles along with their immunomodulatory properties, using Concanavaline A (ConA)-treated rat splenocytes as a model of activated immune cells. Fractions 1 and 2, composed of pure gold nanoparticles, although non-cytotoxic, reduced cellular proliferation. Fraction 2, containing particles smaller in size and lesser agglomerated than fraction 1, up- and down-regulated the production of IL-2 and IL-10, respectively, by activated splenocytes. Fraction 3, containing nanoparticles composed of Au and up to 3 at.% Cu, was non-cytotoxic, but reduced IL-2 production and cell proliferation. Fractions 4 and 5, contaminated with alloying elements from the gold scrap, were cytotoxic. The extent of cytotoxicity and subsequent reduction of cytokine production, as well as the mode ...of cell death, depended on their composition. In conclusion, we showed that USP enables the synthesis of gold nanoparticles, which could be suitable for various biological applications, and that ConA-treated splenocytes represent a reliable model for fast and accurate evaluation of the immunotoxicological profiles of these particles. However, it is necessary to improve this technology and investigate further some of the immunomodulatory mechanisms using more specific immunological tests.
Keywords:
Ultrasonic Spray Pirolysis / Gold Nanoparticles / Cytotoxicity / Cytokines / Cellular ProliferationSource:
Journal of Biomedical Nanotechnology, 2012, 8, 3, 528-538Publisher:
- Amer Scientific Publishers, Valencia
Funding / projects:
- EUREKA [E!4953]
- Application of functionalyzed carbon nanotubes and gold nanoparticles for preparation of dendritic cells for tumor therapy (RS-MESTD-Basic Research (BR or ON)-175102)
- Ministry of Higher Education, Science and Technology of Slovenia
- Slovenian Research Agency
- Slovene Human Resources Development and Scholarship Fund
DOI: 10.1166/jbn.2012.1405
ISSN: 1550-7033
PubMed: 22764424
WoS: 000305093600020
Scopus: 2-s2.0-84862280399
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Institution/Community
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Đokić, Jelena AU - Rudolf, Rebeka AU - Tomić, Sergej AU - Stopić, Srecko AU - Friedrich, Bernd AU - Budić, Bojan AU - Anzel, Ivan AU - Čolić, Miodrag PY - 2012 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/559 AB - We prepared 5 different fractions of nanoparticles from the gold scrap, by using a new technology, Ultrasonic Spray Pirolysis (USP). The aim of this study was to characterize the microstructure and cytotoxicity of the nanoparticles along with their immunomodulatory properties, using Concanavaline A (ConA)-treated rat splenocytes as a model of activated immune cells. Fractions 1 and 2, composed of pure gold nanoparticles, although non-cytotoxic, reduced cellular proliferation. Fraction 2, containing particles smaller in size and lesser agglomerated than fraction 1, up- and down-regulated the production of IL-2 and IL-10, respectively, by activated splenocytes. Fraction 3, containing nanoparticles composed of Au and up to 3 at.% Cu, was non-cytotoxic, but reduced IL-2 production and cell proliferation. Fractions 4 and 5, contaminated with alloying elements from the gold scrap, were cytotoxic. The extent of cytotoxicity and subsequent reduction of cytokine production, as well as the mode of cell death, depended on their composition. In conclusion, we showed that USP enables the synthesis of gold nanoparticles, which could be suitable for various biological applications, and that ConA-treated splenocytes represent a reliable model for fast and accurate evaluation of the immunotoxicological profiles of these particles. However, it is necessary to improve this technology and investigate further some of the immunomodulatory mechanisms using more specific immunological tests. PB - Amer Scientific Publishers, Valencia T2 - Journal of Biomedical Nanotechnology T1 - Immunomodulatory Properties of Nanoparticles Obtained by Ultrasonic Spray Pirolysis from Gold Scrap EP - 538 IS - 3 SP - 528 VL - 8 DO - 10.1166/jbn.2012.1405 ER -
@article{ author = "Đokić, Jelena and Rudolf, Rebeka and Tomić, Sergej and Stopić, Srecko and Friedrich, Bernd and Budić, Bojan and Anzel, Ivan and Čolić, Miodrag", year = "2012", abstract = "We prepared 5 different fractions of nanoparticles from the gold scrap, by using a new technology, Ultrasonic Spray Pirolysis (USP). The aim of this study was to characterize the microstructure and cytotoxicity of the nanoparticles along with their immunomodulatory properties, using Concanavaline A (ConA)-treated rat splenocytes as a model of activated immune cells. Fractions 1 and 2, composed of pure gold nanoparticles, although non-cytotoxic, reduced cellular proliferation. Fraction 2, containing particles smaller in size and lesser agglomerated than fraction 1, up- and down-regulated the production of IL-2 and IL-10, respectively, by activated splenocytes. Fraction 3, containing nanoparticles composed of Au and up to 3 at.% Cu, was non-cytotoxic, but reduced IL-2 production and cell proliferation. Fractions 4 and 5, contaminated with alloying elements from the gold scrap, were cytotoxic. The extent of cytotoxicity and subsequent reduction of cytokine production, as well as the mode of cell death, depended on their composition. In conclusion, we showed that USP enables the synthesis of gold nanoparticles, which could be suitable for various biological applications, and that ConA-treated splenocytes represent a reliable model for fast and accurate evaluation of the immunotoxicological profiles of these particles. However, it is necessary to improve this technology and investigate further some of the immunomodulatory mechanisms using more specific immunological tests.", publisher = "Amer Scientific Publishers, Valencia", journal = "Journal of Biomedical Nanotechnology", title = "Immunomodulatory Properties of Nanoparticles Obtained by Ultrasonic Spray Pirolysis from Gold Scrap", pages = "538-528", number = "3", volume = "8", doi = "10.1166/jbn.2012.1405" }
Đokić, J., Rudolf, R., Tomić, S., Stopić, S., Friedrich, B., Budić, B., Anzel, I.,& Čolić, M.. (2012). Immunomodulatory Properties of Nanoparticles Obtained by Ultrasonic Spray Pirolysis from Gold Scrap. in Journal of Biomedical Nanotechnology Amer Scientific Publishers, Valencia., 8(3), 528-538. https://doi.org/10.1166/jbn.2012.1405
Đokić J, Rudolf R, Tomić S, Stopić S, Friedrich B, Budić B, Anzel I, Čolić M. Immunomodulatory Properties of Nanoparticles Obtained by Ultrasonic Spray Pirolysis from Gold Scrap. in Journal of Biomedical Nanotechnology. 2012;8(3):528-538. doi:10.1166/jbn.2012.1405 .
Đokić, Jelena, Rudolf, Rebeka, Tomić, Sergej, Stopić, Srecko, Friedrich, Bernd, Budić, Bojan, Anzel, Ivan, Čolić, Miodrag, "Immunomodulatory Properties of Nanoparticles Obtained by Ultrasonic Spray Pirolysis from Gold Scrap" in Journal of Biomedical Nanotechnology, 8, no. 3 (2012):528-538, https://doi.org/10.1166/jbn.2012.1405 . .