Genetic Alterations in SMAD4 and K-ras in Serbian Patients With Endometrial Carcinoma
Само за регистроване кориснике
2012
Аутори
Nikolić, AleksandraRistanović, Momcilo
Perović, Vladimir
Trifunović, Jovanka
Perović, Milan
Radojković, Dragica
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Objective: This study was aimed at analyzing alterations in K-ras gene and SMAD4 gene promoter in endometrial carcinoma tissue in Serbian patients. Methods/Materials: The study has encompassed 36 patients whose endometrial cancer tissue samples and peripheral blood samples were analyzed for the presence of alterations in the K-ras gene and the SMAD4 gene promoter. The detection of K-ras codon 12 mutation was performed by polymerase chain reaction restriction fragment length polymorphism technique. Analysis of mononucleotide repeat variants at -462T(15) and -4T(12) of the SMAD4 gene promoter was performed by capillary electrophoresis analysis of DNA fragments fluorescently labeled by polymerase chain reaction. Results: Mutation in codon 12 of the K-ras gene was detected with relatively high frequency of 75.0% (27 of 36 cases). Analysis of 2 mononucleotide repeats in the SMAD4 gene promoter showed that in most cases, haplotypes -462T(15)/-4T(12) and -462T(16)/-4T(12) were present; wherea...s in one case, a novel haplotype - 462T( 15)/- 4T( 10) was detected. Conclusions: Findings on the role and potential significance of the K-ras codon 12 mutation and SMAD4 gene promoter variants in patients with endometrial carcinoma remain controversial, and their occurrence in this type of cancer should be further investigated.
Кључне речи:
SMAD4 / K-ras / Endometrial cancerИзвор:
International Journal of Gynecological Cancer, 2012, 22, 3, 442-446Издавач:
- BMJ Publishing Group, London
Финансирање / пројекти:
- Комплексне болести као модел систем за проучавање модулације фенотипа-структурна и функционална анализа молекуларних биомаркера (RS-MESTD-Basic Research (BR or ON)-173008)
DOI: 10.1097/IGC.0b013e31823fabab
ISSN: 1048-891X
PubMed: 22266936
WoS: 000300735900016
Scopus: 2-s2.0-84858256673
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Nikolić, Aleksandra AU - Ristanović, Momcilo AU - Perović, Vladimir AU - Trifunović, Jovanka AU - Perović, Milan AU - Radojković, Dragica PY - 2012 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/566 AB - Objective: This study was aimed at analyzing alterations in K-ras gene and SMAD4 gene promoter in endometrial carcinoma tissue in Serbian patients. Methods/Materials: The study has encompassed 36 patients whose endometrial cancer tissue samples and peripheral blood samples were analyzed for the presence of alterations in the K-ras gene and the SMAD4 gene promoter. The detection of K-ras codon 12 mutation was performed by polymerase chain reaction restriction fragment length polymorphism technique. Analysis of mononucleotide repeat variants at -462T(15) and -4T(12) of the SMAD4 gene promoter was performed by capillary electrophoresis analysis of DNA fragments fluorescently labeled by polymerase chain reaction. Results: Mutation in codon 12 of the K-ras gene was detected with relatively high frequency of 75.0% (27 of 36 cases). Analysis of 2 mononucleotide repeats in the SMAD4 gene promoter showed that in most cases, haplotypes -462T(15)/-4T(12) and -462T(16)/-4T(12) were present; whereas in one case, a novel haplotype - 462T( 15)/- 4T( 10) was detected. Conclusions: Findings on the role and potential significance of the K-ras codon 12 mutation and SMAD4 gene promoter variants in patients with endometrial carcinoma remain controversial, and their occurrence in this type of cancer should be further investigated. PB - BMJ Publishing Group, London T2 - International Journal of Gynecological Cancer T1 - Genetic Alterations in SMAD4 and K-ras in Serbian Patients With Endometrial Carcinoma EP - 446 IS - 3 SP - 442 VL - 22 DO - 10.1097/IGC.0b013e31823fabab ER -
@article{ author = "Nikolić, Aleksandra and Ristanović, Momcilo and Perović, Vladimir and Trifunović, Jovanka and Perović, Milan and Radojković, Dragica", year = "2012", abstract = "Objective: This study was aimed at analyzing alterations in K-ras gene and SMAD4 gene promoter in endometrial carcinoma tissue in Serbian patients. Methods/Materials: The study has encompassed 36 patients whose endometrial cancer tissue samples and peripheral blood samples were analyzed for the presence of alterations in the K-ras gene and the SMAD4 gene promoter. The detection of K-ras codon 12 mutation was performed by polymerase chain reaction restriction fragment length polymorphism technique. Analysis of mononucleotide repeat variants at -462T(15) and -4T(12) of the SMAD4 gene promoter was performed by capillary electrophoresis analysis of DNA fragments fluorescently labeled by polymerase chain reaction. Results: Mutation in codon 12 of the K-ras gene was detected with relatively high frequency of 75.0% (27 of 36 cases). Analysis of 2 mononucleotide repeats in the SMAD4 gene promoter showed that in most cases, haplotypes -462T(15)/-4T(12) and -462T(16)/-4T(12) were present; whereas in one case, a novel haplotype - 462T( 15)/- 4T( 10) was detected. Conclusions: Findings on the role and potential significance of the K-ras codon 12 mutation and SMAD4 gene promoter variants in patients with endometrial carcinoma remain controversial, and their occurrence in this type of cancer should be further investigated.", publisher = "BMJ Publishing Group, London", journal = "International Journal of Gynecological Cancer", title = "Genetic Alterations in SMAD4 and K-ras in Serbian Patients With Endometrial Carcinoma", pages = "446-442", number = "3", volume = "22", doi = "10.1097/IGC.0b013e31823fabab" }
Nikolić, A., Ristanović, M., Perović, V., Trifunović, J., Perović, M.,& Radojković, D.. (2012). Genetic Alterations in SMAD4 and K-ras in Serbian Patients With Endometrial Carcinoma. in International Journal of Gynecological Cancer BMJ Publishing Group, London., 22(3), 442-446. https://doi.org/10.1097/IGC.0b013e31823fabab
Nikolić A, Ristanović M, Perović V, Trifunović J, Perović M, Radojković D. Genetic Alterations in SMAD4 and K-ras in Serbian Patients With Endometrial Carcinoma. in International Journal of Gynecological Cancer. 2012;22(3):442-446. doi:10.1097/IGC.0b013e31823fabab .
Nikolić, Aleksandra, Ristanović, Momcilo, Perović, Vladimir, Trifunović, Jovanka, Perović, Milan, Radojković, Dragica, "Genetic Alterations in SMAD4 and K-ras in Serbian Patients With Endometrial Carcinoma" in International Journal of Gynecological Cancer, 22, no. 3 (2012):442-446, https://doi.org/10.1097/IGC.0b013e31823fabab . .