Probiotic features of two oral lactobacillus isolates
Autori
Zavisić, GordanaPetricević, Sasa
Radulović, Zeljka
Begović, Jelena
Golić, Nataša
Topisirović, Ljubiša
Strahinić, Ivana
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
In this study, we checked lactobacilli strains of human origin for their potential as probiotic. Samples were collected from oral mucosa of 16 healthy individuals, out of which twenty isolates were obtained and two of them were selected and identified as Lactobacillus plantarum (G1) and L. casei (G3). Both isolates exhibited antagonistic action towards pathogenic microorganisms such as Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Salmonella abony, and Clostridium sporogenes, but not on the growth of Candida albicans. The bacteriocin activity against Staphylococcus aureus ATCC 6358-P was shown only by L. plantarum G1. Moreover, the isolates G1 and G3 showed good viability in the acid gastric environment and in the gut environment containing bovine bile salts. The viability of G1 and G3 isolates in the gastrointestinal tract, and the adhesion to the intestinal mucosa were also confirmed in vivo. The biochemical tests of blood samples revealed lower levels of serum tri...glycerides and cholesterol, as well as reduced activity of alkaline phosphatase in all lactobacilli-treated Wistar rats, compared to control ones. No toxicity for NMRI Ham mice was observed. According to our experimental results, these findings imply that L. plantarum G1 and L. casei G3 could be characterized as potential probiotics.
Ključne reči:
probiotics / Lactobacillus / hipolypemic effect / antimicrobial activityIzvor:
Brazilian Journal of Microbiology, 2012, 43, 1, 418-428Izdavač:
- Sociedade Brasileira de Microbiologia
Finansiranje / projekti:
- 451-01-0065/2008-01/28
- Izučavanje gena i molekularnih mehanizama u osnovi probiotičke aktivnosti bakterija mlečne kiseline izolovanih sa područja zapadnog Balkana (RS-MESTD-Basic Research (BR or ON)-173019)
DOI: 10.1590/S1517-83822012000100050
ISSN: 1517-8382
WoS: 000303939800050
Scopus: 2-s2.0-84861389690
Institucija/grupa
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Zavisić, Gordana AU - Petricević, Sasa AU - Radulović, Zeljka AU - Begović, Jelena AU - Golić, Nataša AU - Topisirović, Ljubiša AU - Strahinić, Ivana PY - 2012 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/571 AB - In this study, we checked lactobacilli strains of human origin for their potential as probiotic. Samples were collected from oral mucosa of 16 healthy individuals, out of which twenty isolates were obtained and two of them were selected and identified as Lactobacillus plantarum (G1) and L. casei (G3). Both isolates exhibited antagonistic action towards pathogenic microorganisms such as Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Salmonella abony, and Clostridium sporogenes, but not on the growth of Candida albicans. The bacteriocin activity against Staphylococcus aureus ATCC 6358-P was shown only by L. plantarum G1. Moreover, the isolates G1 and G3 showed good viability in the acid gastric environment and in the gut environment containing bovine bile salts. The viability of G1 and G3 isolates in the gastrointestinal tract, and the adhesion to the intestinal mucosa were also confirmed in vivo. The biochemical tests of blood samples revealed lower levels of serum triglycerides and cholesterol, as well as reduced activity of alkaline phosphatase in all lactobacilli-treated Wistar rats, compared to control ones. No toxicity for NMRI Ham mice was observed. According to our experimental results, these findings imply that L. plantarum G1 and L. casei G3 could be characterized as potential probiotics. PB - Sociedade Brasileira de Microbiologia T2 - Brazilian Journal of Microbiology T1 - Probiotic features of two oral lactobacillus isolates EP - 428 IS - 1 SP - 418 VL - 43 DO - 10.1590/S1517-83822012000100050 ER -
@article{ author = "Zavisić, Gordana and Petricević, Sasa and Radulović, Zeljka and Begović, Jelena and Golić, Nataša and Topisirović, Ljubiša and Strahinić, Ivana", year = "2012", abstract = "In this study, we checked lactobacilli strains of human origin for their potential as probiotic. Samples were collected from oral mucosa of 16 healthy individuals, out of which twenty isolates were obtained and two of them were selected and identified as Lactobacillus plantarum (G1) and L. casei (G3). Both isolates exhibited antagonistic action towards pathogenic microorganisms such as Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Salmonella abony, and Clostridium sporogenes, but not on the growth of Candida albicans. The bacteriocin activity against Staphylococcus aureus ATCC 6358-P was shown only by L. plantarum G1. Moreover, the isolates G1 and G3 showed good viability in the acid gastric environment and in the gut environment containing bovine bile salts. The viability of G1 and G3 isolates in the gastrointestinal tract, and the adhesion to the intestinal mucosa were also confirmed in vivo. The biochemical tests of blood samples revealed lower levels of serum triglycerides and cholesterol, as well as reduced activity of alkaline phosphatase in all lactobacilli-treated Wistar rats, compared to control ones. No toxicity for NMRI Ham mice was observed. According to our experimental results, these findings imply that L. plantarum G1 and L. casei G3 could be characterized as potential probiotics.", publisher = "Sociedade Brasileira de Microbiologia", journal = "Brazilian Journal of Microbiology", title = "Probiotic features of two oral lactobacillus isolates", pages = "428-418", number = "1", volume = "43", doi = "10.1590/S1517-83822012000100050" }
Zavisić, G., Petricević, S., Radulović, Z., Begović, J., Golić, N., Topisirović, L.,& Strahinić, I.. (2012). Probiotic features of two oral lactobacillus isolates. in Brazilian Journal of Microbiology Sociedade Brasileira de Microbiologia., 43(1), 418-428. https://doi.org/10.1590/S1517-83822012000100050
Zavisić G, Petricević S, Radulović Z, Begović J, Golić N, Topisirović L, Strahinić I. Probiotic features of two oral lactobacillus isolates. in Brazilian Journal of Microbiology. 2012;43(1):418-428. doi:10.1590/S1517-83822012000100050 .
Zavisić, Gordana, Petricević, Sasa, Radulović, Zeljka, Begović, Jelena, Golić, Nataša, Topisirović, Ljubiša, Strahinić, Ivana, "Probiotic features of two oral lactobacillus isolates" in Brazilian Journal of Microbiology, 43, no. 1 (2012):418-428, https://doi.org/10.1590/S1517-83822012000100050 . .