Prevalence of quinolone resistance and mutations in the topoisomerase genes in Salmonella enterica serotype Enteritidis isolates from Serbia
Samo za registrovane korisnike
2012
Autori
Kozoderović, GordanaVelhner, Maja
Jelesić, Zora
Golić, Nataša
Lozo, Jelena
Kehrenberg, Corinna
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
The prevalence of quinolone resistance was studied in Salmonella enterica serotype Enteritidis isolates collected during 2005-2010 in Southern Ba. cka County, Serbia. A total of 878 clinical isolates were examined, among which 19 (2.2%) nalidixic acid (NAL)-resistant S. Enteritidis were detected by selection on agar plates containing 64 mg/L NAL. Antimicrobial susceptibility of the isolates was tested by the agar dilution method. According to Clinical and Laboratory Standards Institute (CLSI) breakpoints, ciprofloxacin (CIP) resistance was not present in the strains. Multiple drug resistance was rare, and resistance to NAL was most often present as a single resistance property. All but one NAL-resistant S. Enteritidis showed reduced susceptibility to CIP [i.e. minimum inhibitory concentration (MIC) gt = 0.125 mg/L]. This isolate of human origin had a CIP MIC of 0.064 mg/L and DNA sequencing revealed that it contained an Asp87Gly gyrA mutation. Most of the remaining isolates had MICs f...or NAL and CIP of 256 mg/L and 0.256 mg/L, respectively. Mutations in the Asp87 codon resulted in substitutions to Asn in most of the isolates, but Asp87Gly and Ser83Phe exchanges were also detected. No mutations were present in the gyrB, parC or parE genes. Although CIP resistance was absent, reduced susceptibility characterised by mutations in gyrA was apparent among S. Enteritidis isolates from Serbia.
Ključne reči:
Salmonella Enteritidis / Resistance / Quinolones / QRDR / gyrAIzvor:
International Journal of Antimicrobial Agents, 2012, 40, 5, 455-457Izdavač:
- Elsevier, Amsterdam
Finansiranje / projekti:
- Istraživanje farmakoloških karakteristika antimikrobnih agenasa, uvođenje novih tehnoloških rešenja i alternativnih metoda profilakse s ciljem da se poboljša kontrola infektivnih oboljenja domaćih životinja (RS-MESTD-Technological Development (TD or TR)-31071)
- Izučavanje gena i molekularnih mehanizama u osnovi probiotičke aktivnosti bakterija mlečne kiseline izolovanih sa područja zapadnog Balkana (RS-MESTD-Basic Research (BR or ON)-173019)
DOI: 10.1016/j.ijantimicag.2012.07.012
ISSN: 0924-8579
PubMed: 22999768
WoS: 000311030700013
Scopus: 2-s2.0-84867397981
Institucija/grupa
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Kozoderović, Gordana AU - Velhner, Maja AU - Jelesić, Zora AU - Golić, Nataša AU - Lozo, Jelena AU - Kehrenberg, Corinna PY - 2012 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/583 AB - The prevalence of quinolone resistance was studied in Salmonella enterica serotype Enteritidis isolates collected during 2005-2010 in Southern Ba. cka County, Serbia. A total of 878 clinical isolates were examined, among which 19 (2.2%) nalidixic acid (NAL)-resistant S. Enteritidis were detected by selection on agar plates containing 64 mg/L NAL. Antimicrobial susceptibility of the isolates was tested by the agar dilution method. According to Clinical and Laboratory Standards Institute (CLSI) breakpoints, ciprofloxacin (CIP) resistance was not present in the strains. Multiple drug resistance was rare, and resistance to NAL was most often present as a single resistance property. All but one NAL-resistant S. Enteritidis showed reduced susceptibility to CIP [i.e. minimum inhibitory concentration (MIC) gt = 0.125 mg/L]. This isolate of human origin had a CIP MIC of 0.064 mg/L and DNA sequencing revealed that it contained an Asp87Gly gyrA mutation. Most of the remaining isolates had MICs for NAL and CIP of 256 mg/L and 0.256 mg/L, respectively. Mutations in the Asp87 codon resulted in substitutions to Asn in most of the isolates, but Asp87Gly and Ser83Phe exchanges were also detected. No mutations were present in the gyrB, parC or parE genes. Although CIP resistance was absent, reduced susceptibility characterised by mutations in gyrA was apparent among S. Enteritidis isolates from Serbia. PB - Elsevier, Amsterdam T2 - International Journal of Antimicrobial Agents T1 - Prevalence of quinolone resistance and mutations in the topoisomerase genes in Salmonella enterica serotype Enteritidis isolates from Serbia EP - 457 IS - 5 SP - 455 VL - 40 DO - 10.1016/j.ijantimicag.2012.07.012 ER -
@article{ author = "Kozoderović, Gordana and Velhner, Maja and Jelesić, Zora and Golić, Nataša and Lozo, Jelena and Kehrenberg, Corinna", year = "2012", abstract = "The prevalence of quinolone resistance was studied in Salmonella enterica serotype Enteritidis isolates collected during 2005-2010 in Southern Ba. cka County, Serbia. A total of 878 clinical isolates were examined, among which 19 (2.2%) nalidixic acid (NAL)-resistant S. Enteritidis were detected by selection on agar plates containing 64 mg/L NAL. Antimicrobial susceptibility of the isolates was tested by the agar dilution method. According to Clinical and Laboratory Standards Institute (CLSI) breakpoints, ciprofloxacin (CIP) resistance was not present in the strains. Multiple drug resistance was rare, and resistance to NAL was most often present as a single resistance property. All but one NAL-resistant S. Enteritidis showed reduced susceptibility to CIP [i.e. minimum inhibitory concentration (MIC) gt = 0.125 mg/L]. This isolate of human origin had a CIP MIC of 0.064 mg/L and DNA sequencing revealed that it contained an Asp87Gly gyrA mutation. Most of the remaining isolates had MICs for NAL and CIP of 256 mg/L and 0.256 mg/L, respectively. Mutations in the Asp87 codon resulted in substitutions to Asn in most of the isolates, but Asp87Gly and Ser83Phe exchanges were also detected. No mutations were present in the gyrB, parC or parE genes. Although CIP resistance was absent, reduced susceptibility characterised by mutations in gyrA was apparent among S. Enteritidis isolates from Serbia.", publisher = "Elsevier, Amsterdam", journal = "International Journal of Antimicrobial Agents", title = "Prevalence of quinolone resistance and mutations in the topoisomerase genes in Salmonella enterica serotype Enteritidis isolates from Serbia", pages = "457-455", number = "5", volume = "40", doi = "10.1016/j.ijantimicag.2012.07.012" }
Kozoderović, G., Velhner, M., Jelesić, Z., Golić, N., Lozo, J.,& Kehrenberg, C.. (2012). Prevalence of quinolone resistance and mutations in the topoisomerase genes in Salmonella enterica serotype Enteritidis isolates from Serbia. in International Journal of Antimicrobial Agents Elsevier, Amsterdam., 40(5), 455-457. https://doi.org/10.1016/j.ijantimicag.2012.07.012
Kozoderović G, Velhner M, Jelesić Z, Golić N, Lozo J, Kehrenberg C. Prevalence of quinolone resistance and mutations in the topoisomerase genes in Salmonella enterica serotype Enteritidis isolates from Serbia. in International Journal of Antimicrobial Agents. 2012;40(5):455-457. doi:10.1016/j.ijantimicag.2012.07.012 .
Kozoderović, Gordana, Velhner, Maja, Jelesić, Zora, Golić, Nataša, Lozo, Jelena, Kehrenberg, Corinna, "Prevalence of quinolone resistance and mutations in the topoisomerase genes in Salmonella enterica serotype Enteritidis isolates from Serbia" in International Journal of Antimicrobial Agents, 40, no. 5 (2012):455-457, https://doi.org/10.1016/j.ijantimicag.2012.07.012 . .