Macrophage migration inhibitory factor (MIF) enhances palmitic acid-and glucose-induced murine beta cell dysfunction and destruction in vitro
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2012
Authors
Stojanović, IvanaSaksida, Tamara
Timotijević, Gordana
Sandler, Stellan
Stošić-Grujičić, Stanislava
Article (Published version)
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Although several reports suggest a potentially deleterious role of macrophage migration inhibitory factor (MIF) in type 2 diabetes (T2D) pathology, it is still unclear how this pro-inflammatory cytokine acts on pancreatic beta cells. The aim of the present study was to evaluate MIF effects on murine beta cells in the in vitro settings mimicking T2D-associated conditions. Results indicate that recombinant MIF further increased apoptosis of pancreatic islets or MIN6 cells upon exposure to palmitic acid or glucose. This was accompanied by upregulation of several pro-apoptotic molecules. Furthermore, MIF potentiated nutrient-induced islet cell dysfunction, as revealed by lower glucose oxidation rate, ATP content, and depolarized mitochondrial membrane. The final outcome was potentiation of mitochondrial apoptotic pathway. The observed upregulation of nutrient-induced islet cell dysfunction and apoptosis by MIF implicates that silencing MIF may be beneficial for maintaining integrity of end...ocrine pancreas in obesity-associated T2D.
Keywords:
type 2 diabetes / pancreatic islets / palmitic acid / Macrophage migration inhibitory factor / glucose / apoptosisSource:
Growth Factors, 2012, 30, 6, 385-393Publisher:
- Taylor & Francis Ltd, Abingdon
Funding / projects:
- EASD/AstraZeneca
- Molecular mechanisms of physiological and pharmacological control of inflammation and cancer (RS-MESTD-Basic Research (BR or ON)-173013)
DOI: 10.3109/08977194.2012.734506
ISSN: 0897-7194
PubMed: 23137174
WoS: 000312222900003
Scopus: 2-s2.0-84871040863
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Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Stojanović, Ivana AU - Saksida, Tamara AU - Timotijević, Gordana AU - Sandler, Stellan AU - Stošić-Grujičić, Stanislava PY - 2012 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/599 AB - Although several reports suggest a potentially deleterious role of macrophage migration inhibitory factor (MIF) in type 2 diabetes (T2D) pathology, it is still unclear how this pro-inflammatory cytokine acts on pancreatic beta cells. The aim of the present study was to evaluate MIF effects on murine beta cells in the in vitro settings mimicking T2D-associated conditions. Results indicate that recombinant MIF further increased apoptosis of pancreatic islets or MIN6 cells upon exposure to palmitic acid or glucose. This was accompanied by upregulation of several pro-apoptotic molecules. Furthermore, MIF potentiated nutrient-induced islet cell dysfunction, as revealed by lower glucose oxidation rate, ATP content, and depolarized mitochondrial membrane. The final outcome was potentiation of mitochondrial apoptotic pathway. The observed upregulation of nutrient-induced islet cell dysfunction and apoptosis by MIF implicates that silencing MIF may be beneficial for maintaining integrity of endocrine pancreas in obesity-associated T2D. PB - Taylor & Francis Ltd, Abingdon T2 - Growth Factors T1 - Macrophage migration inhibitory factor (MIF) enhances palmitic acid-and glucose-induced murine beta cell dysfunction and destruction in vitro EP - 393 IS - 6 SP - 385 VL - 30 DO - 10.3109/08977194.2012.734506 ER -
@article{ author = "Stojanović, Ivana and Saksida, Tamara and Timotijević, Gordana and Sandler, Stellan and Stošić-Grujičić, Stanislava", year = "2012", abstract = "Although several reports suggest a potentially deleterious role of macrophage migration inhibitory factor (MIF) in type 2 diabetes (T2D) pathology, it is still unclear how this pro-inflammatory cytokine acts on pancreatic beta cells. The aim of the present study was to evaluate MIF effects on murine beta cells in the in vitro settings mimicking T2D-associated conditions. Results indicate that recombinant MIF further increased apoptosis of pancreatic islets or MIN6 cells upon exposure to palmitic acid or glucose. This was accompanied by upregulation of several pro-apoptotic molecules. Furthermore, MIF potentiated nutrient-induced islet cell dysfunction, as revealed by lower glucose oxidation rate, ATP content, and depolarized mitochondrial membrane. The final outcome was potentiation of mitochondrial apoptotic pathway. The observed upregulation of nutrient-induced islet cell dysfunction and apoptosis by MIF implicates that silencing MIF may be beneficial for maintaining integrity of endocrine pancreas in obesity-associated T2D.", publisher = "Taylor & Francis Ltd, Abingdon", journal = "Growth Factors", title = "Macrophage migration inhibitory factor (MIF) enhances palmitic acid-and glucose-induced murine beta cell dysfunction and destruction in vitro", pages = "393-385", number = "6", volume = "30", doi = "10.3109/08977194.2012.734506" }
Stojanović, I., Saksida, T., Timotijević, G., Sandler, S.,& Stošić-Grujičić, S.. (2012). Macrophage migration inhibitory factor (MIF) enhances palmitic acid-and glucose-induced murine beta cell dysfunction and destruction in vitro. in Growth Factors Taylor & Francis Ltd, Abingdon., 30(6), 385-393. https://doi.org/10.3109/08977194.2012.734506
Stojanović I, Saksida T, Timotijević G, Sandler S, Stošić-Grujičić S. Macrophage migration inhibitory factor (MIF) enhances palmitic acid-and glucose-induced murine beta cell dysfunction and destruction in vitro. in Growth Factors. 2012;30(6):385-393. doi:10.3109/08977194.2012.734506 .
Stojanović, Ivana, Saksida, Tamara, Timotijević, Gordana, Sandler, Stellan, Stošić-Grujičić, Stanislava, "Macrophage migration inhibitory factor (MIF) enhances palmitic acid-and glucose-induced murine beta cell dysfunction and destruction in vitro" in Growth Factors, 30, no. 6 (2012):385-393, https://doi.org/10.3109/08977194.2012.734506 . .