A Simple and Efficient DNA Isolation Method for Salvia officinalis
Abstract
We report an efficient, simple, and cost-effective protocol for the isolation of genomic DNA from an aromatic medicinal plant, common sage (Salvia officinalis L.). Our modification of the standard CTAB protocol includes two polyphenol adsorbents (PVP 10 and activated charcoal), high NaCl concentrations (4 M) for removing polysaccharides, and repeated Sevag treatment to remove proteins and other carbohydrate contaminants. The mean DNA yield obtained with our Protocol 2 was 330.6 mu g DNA g(-1) of dry leaf tissue, and the absorbance ratios 260/280 and 260/230 nm averaged 1.909 and 1.894, respectively, revealing lack of contamination. PCR amplifications of one nuclear (26S rDNA) and one chloroplast (rps16-trnK) locus indicated that our DNA isolation protocol may be used in common sage and other aromatic and medicinal plants containing essential oil for molecular biologic and biotechnological studies and for population genetics, phylogeographic, and conservation surveys in which nuclear or... chloroplast genomes would be studied in large numbers of individuals.
Keywords:
Sevag / Salvia officinalis / rps16-trnK / PVP 10 / PCR / NaCl / DNA isolation / CTAB / Activated charcoal / 26S rDNASource:
Biochemical Genetics, 2012, 50, 11-12, 881-892Publisher:
- Springer/Plenum Publishers, New York
Funding / projects:
- Plant Biodiversity of Serbia and the Balkans - assesment, sustainable use and protection (RS-MESTD-Basic Research (BR or ON)-173030)
- Investigation on the medicinal plants: morphological, chemical and pharmacological characterisation (RS-MESTD-Basic Research (BR or ON)-173021)
DOI: 10.1007/s10528-012-9528-y
ISSN: 0006-2928
PubMed: 22847363
WoS: 000310887500006
Scopus: 2-s2.0-84871194909
Collections
Institution/Community
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Aleksić, Jelena M. AU - Stojanović, Danilo AU - Banović Đeri, Bojana AU - Jancić, Radisa PY - 2012 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/606 AB - We report an efficient, simple, and cost-effective protocol for the isolation of genomic DNA from an aromatic medicinal plant, common sage (Salvia officinalis L.). Our modification of the standard CTAB protocol includes two polyphenol adsorbents (PVP 10 and activated charcoal), high NaCl concentrations (4 M) for removing polysaccharides, and repeated Sevag treatment to remove proteins and other carbohydrate contaminants. The mean DNA yield obtained with our Protocol 2 was 330.6 mu g DNA g(-1) of dry leaf tissue, and the absorbance ratios 260/280 and 260/230 nm averaged 1.909 and 1.894, respectively, revealing lack of contamination. PCR amplifications of one nuclear (26S rDNA) and one chloroplast (rps16-trnK) locus indicated that our DNA isolation protocol may be used in common sage and other aromatic and medicinal plants containing essential oil for molecular biologic and biotechnological studies and for population genetics, phylogeographic, and conservation surveys in which nuclear or chloroplast genomes would be studied in large numbers of individuals. PB - Springer/Plenum Publishers, New York T2 - Biochemical Genetics T1 - A Simple and Efficient DNA Isolation Method for Salvia officinalis EP - 892 IS - 11-12 SP - 881 VL - 50 DO - 10.1007/s10528-012-9528-y ER -
@article{ author = "Aleksić, Jelena M. and Stojanović, Danilo and Banović Đeri, Bojana and Jancić, Radisa", year = "2012", abstract = "We report an efficient, simple, and cost-effective protocol for the isolation of genomic DNA from an aromatic medicinal plant, common sage (Salvia officinalis L.). Our modification of the standard CTAB protocol includes two polyphenol adsorbents (PVP 10 and activated charcoal), high NaCl concentrations (4 M) for removing polysaccharides, and repeated Sevag treatment to remove proteins and other carbohydrate contaminants. The mean DNA yield obtained with our Protocol 2 was 330.6 mu g DNA g(-1) of dry leaf tissue, and the absorbance ratios 260/280 and 260/230 nm averaged 1.909 and 1.894, respectively, revealing lack of contamination. PCR amplifications of one nuclear (26S rDNA) and one chloroplast (rps16-trnK) locus indicated that our DNA isolation protocol may be used in common sage and other aromatic and medicinal plants containing essential oil for molecular biologic and biotechnological studies and for population genetics, phylogeographic, and conservation surveys in which nuclear or chloroplast genomes would be studied in large numbers of individuals.", publisher = "Springer/Plenum Publishers, New York", journal = "Biochemical Genetics", title = "A Simple and Efficient DNA Isolation Method for Salvia officinalis", pages = "892-881", number = "11-12", volume = "50", doi = "10.1007/s10528-012-9528-y" }
Aleksić, J. M., Stojanović, D., Banović Đeri, B.,& Jancić, R.. (2012). A Simple and Efficient DNA Isolation Method for Salvia officinalis. in Biochemical Genetics Springer/Plenum Publishers, New York., 50(11-12), 881-892. https://doi.org/10.1007/s10528-012-9528-y
Aleksić JM, Stojanović D, Banović Đeri B, Jancić R. A Simple and Efficient DNA Isolation Method for Salvia officinalis. in Biochemical Genetics. 2012;50(11-12):881-892. doi:10.1007/s10528-012-9528-y .
Aleksić, Jelena M., Stojanović, Danilo, Banović Đeri, Bojana, Jancić, Radisa, "A Simple and Efficient DNA Isolation Method for Salvia officinalis" in Biochemical Genetics, 50, no. 11-12 (2012):881-892, https://doi.org/10.1007/s10528-012-9528-y . .