Polymerase chain reaction in the identification of periodontopathogens: A reliable and satisfactory method?
2012
Преузимање 🢃
Аутори
Nikolić-Jakoba, NatašaVojnović, Sandra
Pavić, Aleksandar
Janković, S.
Leković, V.
Vasiljević, Branka
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Aggregatibacter actinomycetemcomitans is considered one of the bacterial species of etiological importance in periodontitis. The aim of this study was to evaluate the serotype of A. actinomycetemcomitans in the subgingival biofilm in subjects with periodontal health and disease. Pooled samples of subgingival plaque were taken for culture-based identification of microorganisms. Colonies suspected to be A. actinomycetemcomitans were selected for molecular identification using either multiplex or conventional PCR in serotype-specific genotyping and 16S rRNA gene sequencing. In silico analysis showed that most selected colonies belong to the genus Campylobacter, although positive signals for serotypes of A. actinomycetemcomitans were obtained with these samples. Identification of A. actinomycetemcomitans by conventional PCR for 16S rRNA with one species-specific and one universal primer was inconclusive because an almost identical signal with Campylobacter gracilis was obtained. Although P...CR-based methods for the identification of A. actinomycetemcomitans are more rapid, sequencing should not be omitted.
Кључне речи:
periodontitis / PCR / Aggregatibacter actinomycetemcomitansИзвор:
Archives of Biological Sciences, 2012, 64, 4, 1413-1423Издавач:
- Srpsko biološko društvo, Beograd, i dr.
Финансирање / пројекти:
- Интеракција етиопатогенетских механизама пародонтопатије и паериимплантитиса са системским болестима данашњице (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41008)
- Изучавање микробиолошког диверзитета и карактеризација корисних срединских микроорганизама (RS-MESTD-Basic Research (BR or ON)-173048)
DOI: 10.2298/ABS1204413J
ISSN: 0354-4664
WoS: 000312046200023
Scopus: 2-s2.0-84867784935
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Nikolić-Jakoba, Nataša AU - Vojnović, Sandra AU - Pavić, Aleksandar AU - Janković, S. AU - Leković, V. AU - Vasiljević, Branka PY - 2012 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/608 AB - Aggregatibacter actinomycetemcomitans is considered one of the bacterial species of etiological importance in periodontitis. The aim of this study was to evaluate the serotype of A. actinomycetemcomitans in the subgingival biofilm in subjects with periodontal health and disease. Pooled samples of subgingival plaque were taken for culture-based identification of microorganisms. Colonies suspected to be A. actinomycetemcomitans were selected for molecular identification using either multiplex or conventional PCR in serotype-specific genotyping and 16S rRNA gene sequencing. In silico analysis showed that most selected colonies belong to the genus Campylobacter, although positive signals for serotypes of A. actinomycetemcomitans were obtained with these samples. Identification of A. actinomycetemcomitans by conventional PCR for 16S rRNA with one species-specific and one universal primer was inconclusive because an almost identical signal with Campylobacter gracilis was obtained. Although PCR-based methods for the identification of A. actinomycetemcomitans are more rapid, sequencing should not be omitted. PB - Srpsko biološko društvo, Beograd, i dr. T2 - Archives of Biological Sciences T1 - Polymerase chain reaction in the identification of periodontopathogens: A reliable and satisfactory method? EP - 1423 IS - 4 SP - 1413 VL - 64 DO - 10.2298/ABS1204413J ER -
@article{ author = "Nikolić-Jakoba, Nataša and Vojnović, Sandra and Pavić, Aleksandar and Janković, S. and Leković, V. and Vasiljević, Branka", year = "2012", abstract = "Aggregatibacter actinomycetemcomitans is considered one of the bacterial species of etiological importance in periodontitis. The aim of this study was to evaluate the serotype of A. actinomycetemcomitans in the subgingival biofilm in subjects with periodontal health and disease. Pooled samples of subgingival plaque were taken for culture-based identification of microorganisms. Colonies suspected to be A. actinomycetemcomitans were selected for molecular identification using either multiplex or conventional PCR in serotype-specific genotyping and 16S rRNA gene sequencing. In silico analysis showed that most selected colonies belong to the genus Campylobacter, although positive signals for serotypes of A. actinomycetemcomitans were obtained with these samples. Identification of A. actinomycetemcomitans by conventional PCR for 16S rRNA with one species-specific and one universal primer was inconclusive because an almost identical signal with Campylobacter gracilis was obtained. Although PCR-based methods for the identification of A. actinomycetemcomitans are more rapid, sequencing should not be omitted.", publisher = "Srpsko biološko društvo, Beograd, i dr.", journal = "Archives of Biological Sciences", title = "Polymerase chain reaction in the identification of periodontopathogens: A reliable and satisfactory method?", pages = "1423-1413", number = "4", volume = "64", doi = "10.2298/ABS1204413J" }
Nikolić-Jakoba, N., Vojnović, S., Pavić, A., Janković, S., Leković, V.,& Vasiljević, B.. (2012). Polymerase chain reaction in the identification of periodontopathogens: A reliable and satisfactory method?. in Archives of Biological Sciences Srpsko biološko društvo, Beograd, i dr.., 64(4), 1413-1423. https://doi.org/10.2298/ABS1204413J
Nikolić-Jakoba N, Vojnović S, Pavić A, Janković S, Leković V, Vasiljević B. Polymerase chain reaction in the identification of periodontopathogens: A reliable and satisfactory method?. in Archives of Biological Sciences. 2012;64(4):1413-1423. doi:10.2298/ABS1204413J .
Nikolić-Jakoba, Nataša, Vojnović, Sandra, Pavić, Aleksandar, Janković, S., Leković, V., Vasiljević, Branka, "Polymerase chain reaction in the identification of periodontopathogens: A reliable and satisfactory method?" in Archives of Biological Sciences, 64, no. 4 (2012):1413-1423, https://doi.org/10.2298/ABS1204413J . .