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Production of a chiral alcohol, 1-(3,4-dihydroxyphenyl) ethanol, by mushroom tyrosinase
dc.creator | Brooks, Sarah J. | |
dc.creator | Nikodinović-Runić, Jasmina | |
dc.creator | Martin, Leona | |
dc.creator | Doyle, Evelyn M. | |
dc.creator | O'Sullivan, Timothy | |
dc.creator | Guiry, Patrick J. | |
dc.creator | Coulombel, Lydie | |
dc.creator | Li, Zhi | |
dc.creator | O'Connor, Kevin | |
dc.date.accessioned | 2022-11-15T14:18:41Z | |
dc.date.available | 2022-11-15T14:18:41Z | |
dc.date.issued | 2013 | |
dc.identifier.issn | 0141-5492 | |
dc.identifier.uri | https://imagine.imgge.bg.ac.rs/handle/123456789/624 | |
dc.description.abstract | 1-(3,4-Dihydroxyphenyl) ethanol was produced biocatalytically for the first time using mushroom tyrosinase. 4-Ethylphenol at 1 mM was consumed over 12 min giving 0.23 mM 4-ethylcatechol and 0.36 mM (R/S)-1-(3,4-dihydroxyphenyl) ethanol (ee 0.5 %). Mushroom tyrosinase consumed 4-ethylphenol at 6.7 mu mol min(-1) mg protein(-1) while the rates of formation of 4-ethylcatechol and 1-(3,4-dihydroxyphenyl) ethanol were 1.1 and 1.9 mu mol min(-1) mg protein(-1). Addition of the ascorbic acid, as a reducing agent to biotransformation reactions, increased 4-ethylcatechol formation by 340 %. However, accumulation of 1-(3,4-dihydroxyphenyl) ethanol was not observed in the presence of ascorbic acid. While the 1-(3,4-dihydroxyphenyl) ethanol was racemic, it is the first chiral product produced by tyrosinase starting from a non-chiral substrate. | en |
dc.publisher | Springer, Dordrecht | |
dc.relation | Enterprise Ireland Research Scholarship Programme [BR/1999/043] | |
dc.relation | Society for General Microbiology Presidents Award Scheme | |
dc.relation | Irish Environmental Protection Agency Contributory Scholarship Programme [2001-CS-(17/26)] | |
dc.rights | restrictedAccess | |
dc.source | Biotechnology Letters | |
dc.subject | Tyrosinase | en |
dc.subject | Oxidation | en |
dc.subject | Chiral product | en |
dc.subject | Biotransformation | en |
dc.title | Production of a chiral alcohol, 1-(3,4-dihydroxyphenyl) ethanol, by mushroom tyrosinase | en |
dc.type | article | |
dc.rights.license | ARR | |
dc.citation.epage | 783 | |
dc.citation.issue | 5 | |
dc.citation.other | 35(5): 779-783 | |
dc.citation.rank | M23 | |
dc.citation.spage | 779 | |
dc.citation.volume | 35 | |
dc.identifier.doi | 10.1007/s10529-013-1148-z | |
dc.identifier.pmid | 23355036 | |
dc.identifier.scopus | 2-s2.0-84876989904 | |
dc.identifier.wos | 000318510500017 | |
dc.type.version | publishedVersion |