Apotransferrin inhibits interleukin-2 expression and protects mice from experimental autoimmune encephalomyelitis
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2013
Authors
Saksida, TamaraMiljković, Djordje
Timotijević, Gordana
Stojanović, Ivana
Mijatović, Sanja
Fagone, Paolo
Mangano, Katia
Mammana, Santa
Farina, Claudio
Ascione, Ester
Maiello, Valentina
Nicoletti, Ferdinando
Stošić-Grujičić, Stanislava
Article (Published version)
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Show full item recordAbstract
Transferrin (Tf) has a major role in T cell activation and proliferation. Here, we investigated whether Tf exerts immunomodulatory effects on T cells and in development of T-cell driven experimental autoimmune encephalomyelitis (EAE). While treatment of concanavalin A-stimulated splenocytes with apotransferrin (ApoTf) did not affect release of IL-1 beta, TNF, INF-gamma, IL-17, IL-4, and IL-10, it markedly and dose-dependently down-regulated synthesis of IL-2 in these cells. ApoTf also inhibited IL-2 generation in purified CD3(+) T cells and the effect was accompanied with down-regulation of MAPK p44/42 and NF kappa B signaling. Despite impeded IL-2 release, proliferation of splenocytes was not inhibited by ApoTf. Importantly, ApoTf ameliorated EAE in mice and significantly reduced ex vivo IL-2 production in proteolipid protein-specific lymphocytes. Thus ApoTf may be a promising beneficial agent for multiple sclerosis.
Keywords:
T cell activation / IL-2 / Experimental autoimmune encephalomyelitis / ApotransferrinSource:
Journal of Neuroimmunology, 2013, 262, 1-2, 72-78Publisher:
- Elsevier, Amsterdam
Funding / projects:
- MIUR (Ministry of Education, University, Research) [795]
- Molecular mechanisms of physiological and pharmacological control of inflammation and cancer (RS-MESTD-Basic Research (BR or ON)-173013)
- Cellular and molecular mechanisms of recovery of rats from experimental autoimmune encephalomyelitis (RS-MESTD-Basic Research (BR or ON)-173035)
DOI: 10.1016/j.jneuroim.2013.07.001
ISSN: 0165-5728
PubMed: 23890777
WoS: 000324970400010
Scopus: 2-s2.0-84884138712
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Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Saksida, Tamara AU - Miljković, Djordje AU - Timotijević, Gordana AU - Stojanović, Ivana AU - Mijatović, Sanja AU - Fagone, Paolo AU - Mangano, Katia AU - Mammana, Santa AU - Farina, Claudio AU - Ascione, Ester AU - Maiello, Valentina AU - Nicoletti, Ferdinando AU - Stošić-Grujičić, Stanislava PY - 2013 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/627 AB - Transferrin (Tf) has a major role in T cell activation and proliferation. Here, we investigated whether Tf exerts immunomodulatory effects on T cells and in development of T-cell driven experimental autoimmune encephalomyelitis (EAE). While treatment of concanavalin A-stimulated splenocytes with apotransferrin (ApoTf) did not affect release of IL-1 beta, TNF, INF-gamma, IL-17, IL-4, and IL-10, it markedly and dose-dependently down-regulated synthesis of IL-2 in these cells. ApoTf also inhibited IL-2 generation in purified CD3(+) T cells and the effect was accompanied with down-regulation of MAPK p44/42 and NF kappa B signaling. Despite impeded IL-2 release, proliferation of splenocytes was not inhibited by ApoTf. Importantly, ApoTf ameliorated EAE in mice and significantly reduced ex vivo IL-2 production in proteolipid protein-specific lymphocytes. Thus ApoTf may be a promising beneficial agent for multiple sclerosis. PB - Elsevier, Amsterdam T2 - Journal of Neuroimmunology T1 - Apotransferrin inhibits interleukin-2 expression and protects mice from experimental autoimmune encephalomyelitis EP - 78 IS - 1-2 SP - 72 VL - 262 DO - 10.1016/j.jneuroim.2013.07.001 ER -
@article{ author = "Saksida, Tamara and Miljković, Djordje and Timotijević, Gordana and Stojanović, Ivana and Mijatović, Sanja and Fagone, Paolo and Mangano, Katia and Mammana, Santa and Farina, Claudio and Ascione, Ester and Maiello, Valentina and Nicoletti, Ferdinando and Stošić-Grujičić, Stanislava", year = "2013", abstract = "Transferrin (Tf) has a major role in T cell activation and proliferation. Here, we investigated whether Tf exerts immunomodulatory effects on T cells and in development of T-cell driven experimental autoimmune encephalomyelitis (EAE). While treatment of concanavalin A-stimulated splenocytes with apotransferrin (ApoTf) did not affect release of IL-1 beta, TNF, INF-gamma, IL-17, IL-4, and IL-10, it markedly and dose-dependently down-regulated synthesis of IL-2 in these cells. ApoTf also inhibited IL-2 generation in purified CD3(+) T cells and the effect was accompanied with down-regulation of MAPK p44/42 and NF kappa B signaling. Despite impeded IL-2 release, proliferation of splenocytes was not inhibited by ApoTf. Importantly, ApoTf ameliorated EAE in mice and significantly reduced ex vivo IL-2 production in proteolipid protein-specific lymphocytes. Thus ApoTf may be a promising beneficial agent for multiple sclerosis.", publisher = "Elsevier, Amsterdam", journal = "Journal of Neuroimmunology", title = "Apotransferrin inhibits interleukin-2 expression and protects mice from experimental autoimmune encephalomyelitis", pages = "78-72", number = "1-2", volume = "262", doi = "10.1016/j.jneuroim.2013.07.001" }
Saksida, T., Miljković, D., Timotijević, G., Stojanović, I., Mijatović, S., Fagone, P., Mangano, K., Mammana, S., Farina, C., Ascione, E., Maiello, V., Nicoletti, F.,& Stošić-Grujičić, S.. (2013). Apotransferrin inhibits interleukin-2 expression and protects mice from experimental autoimmune encephalomyelitis. in Journal of Neuroimmunology Elsevier, Amsterdam., 262(1-2), 72-78. https://doi.org/10.1016/j.jneuroim.2013.07.001
Saksida T, Miljković D, Timotijević G, Stojanović I, Mijatović S, Fagone P, Mangano K, Mammana S, Farina C, Ascione E, Maiello V, Nicoletti F, Stošić-Grujičić S. Apotransferrin inhibits interleukin-2 expression and protects mice from experimental autoimmune encephalomyelitis. in Journal of Neuroimmunology. 2013;262(1-2):72-78. doi:10.1016/j.jneuroim.2013.07.001 .
Saksida, Tamara, Miljković, Djordje, Timotijević, Gordana, Stojanović, Ivana, Mijatović, Sanja, Fagone, Paolo, Mangano, Katia, Mammana, Santa, Farina, Claudio, Ascione, Ester, Maiello, Valentina, Nicoletti, Ferdinando, Stošić-Grujičić, Stanislava, "Apotransferrin inhibits interleukin-2 expression and protects mice from experimental autoimmune encephalomyelitis" in Journal of Neuroimmunology, 262, no. 1-2 (2013):72-78, https://doi.org/10.1016/j.jneuroim.2013.07.001 . .