Assessment of Deoxyribonuclease Activity in Biological Samples by a Fluorescence Detection-Based Method
Apstrakt
Objective: To develop a speedy, sensitive, low-cost fluorescence detection assay for assessing deoxyribonuclease activity in biological samples. Methods: Deoxyribonuclease activity was measured in serum samples of 15 patients with lung cancer and 15 healthy individuals. The assay is based on hydrolysis of fluorescently labeled DNA into fragments that are subsequently measured by capillary electrophoresis. Results: Mean (SD) reduction in signal intensity compared with the negative control reaction was 0.04 (0.09) for the group of patients with lung cancer and 0.01 (0.07) for the control group. The difference in signal intensity between the 2 groups was not statistically significant. Conclusion: The main advantages of the fluorescence detection assay are its high sensitivity, the short time required for it to obtain results, and its low cost. The method provides a useful alternative to the conventional enzyme-linked immunosorbent assay (ELISA) and can potentially be applied to a variety ...of studies, especially when assessment of deoxyribonuclease activity in large sample series is required.
Ključne reči:
serum / fluorescence / deoxyribonuclease / capillary electrophoresisIzvor:
Laboratory Medicine, 2013, 44, 2, 125-128Izdavač:
- Amer Soc Clinical Pathology, Chicago
Finansiranje / projekti:
- Kompleksne bolesti kao model sistem za proučavanje modulacije fenotipa-strukturna i funkcionalna analiza molekularnih biomarkera (RS-MESTD-Basic Research (BR or ON)-173008)
DOI: 10.1309/LMD9INNMFDO5XGIW
ISSN: 0007-5027
WoS: 000318058300005
Scopus: 2-s2.0-84881158532
Kolekcije
Institucija/grupa
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Vancevska, Aleksandra AU - Nikolić, Aleksandra PY - 2013 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/639 AB - Objective: To develop a speedy, sensitive, low-cost fluorescence detection assay for assessing deoxyribonuclease activity in biological samples. Methods: Deoxyribonuclease activity was measured in serum samples of 15 patients with lung cancer and 15 healthy individuals. The assay is based on hydrolysis of fluorescently labeled DNA into fragments that are subsequently measured by capillary electrophoresis. Results: Mean (SD) reduction in signal intensity compared with the negative control reaction was 0.04 (0.09) for the group of patients with lung cancer and 0.01 (0.07) for the control group. The difference in signal intensity between the 2 groups was not statistically significant. Conclusion: The main advantages of the fluorescence detection assay are its high sensitivity, the short time required for it to obtain results, and its low cost. The method provides a useful alternative to the conventional enzyme-linked immunosorbent assay (ELISA) and can potentially be applied to a variety of studies, especially when assessment of deoxyribonuclease activity in large sample series is required. PB - Amer Soc Clinical Pathology, Chicago T2 - Laboratory Medicine T1 - Assessment of Deoxyribonuclease Activity in Biological Samples by a Fluorescence Detection-Based Method EP - 128 IS - 2 SP - 125 VL - 44 DO - 10.1309/LMD9INNMFDO5XGIW ER -
@article{ author = "Vancevska, Aleksandra and Nikolić, Aleksandra", year = "2013", abstract = "Objective: To develop a speedy, sensitive, low-cost fluorescence detection assay for assessing deoxyribonuclease activity in biological samples. Methods: Deoxyribonuclease activity was measured in serum samples of 15 patients with lung cancer and 15 healthy individuals. The assay is based on hydrolysis of fluorescently labeled DNA into fragments that are subsequently measured by capillary electrophoresis. Results: Mean (SD) reduction in signal intensity compared with the negative control reaction was 0.04 (0.09) for the group of patients with lung cancer and 0.01 (0.07) for the control group. The difference in signal intensity between the 2 groups was not statistically significant. Conclusion: The main advantages of the fluorescence detection assay are its high sensitivity, the short time required for it to obtain results, and its low cost. The method provides a useful alternative to the conventional enzyme-linked immunosorbent assay (ELISA) and can potentially be applied to a variety of studies, especially when assessment of deoxyribonuclease activity in large sample series is required.", publisher = "Amer Soc Clinical Pathology, Chicago", journal = "Laboratory Medicine", title = "Assessment of Deoxyribonuclease Activity in Biological Samples by a Fluorescence Detection-Based Method", pages = "128-125", number = "2", volume = "44", doi = "10.1309/LMD9INNMFDO5XGIW" }
Vancevska, A.,& Nikolić, A.. (2013). Assessment of Deoxyribonuclease Activity in Biological Samples by a Fluorescence Detection-Based Method. in Laboratory Medicine Amer Soc Clinical Pathology, Chicago., 44(2), 125-128. https://doi.org/10.1309/LMD9INNMFDO5XGIW
Vancevska A, Nikolić A. Assessment of Deoxyribonuclease Activity in Biological Samples by a Fluorescence Detection-Based Method. in Laboratory Medicine. 2013;44(2):125-128. doi:10.1309/LMD9INNMFDO5XGIW .
Vancevska, Aleksandra, Nikolić, Aleksandra, "Assessment of Deoxyribonuclease Activity in Biological Samples by a Fluorescence Detection-Based Method" in Laboratory Medicine, 44, no. 2 (2013):125-128, https://doi.org/10.1309/LMD9INNMFDO5XGIW . .