Saquinavir-NO inhibits S6 kinase activity, impairs secretion of the encephalytogenic cytokines interleukin-17 and interferon-gamma and ameliorates experimental autoimmune encephalomyelitis
Само за регистроване кориснике
2013
Аутори
Petković, FilipBlazevski, Jana
Momcilović, Miljana
Timotijević, Gordana
Zocca, Mai-Britt
Mijatović, Sanja
Maksimović-Ivanić, Danijela
Mangano, Katia
Fagone, Paolo
Stošić-Grujičić, Stanislava
Nicoletti, Ferdinando
Miljković, Djordje
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
NO-hybridization of the HIV protease inhibitor Saquinavir generates a new chemical entity named Saq-NO, that retains the anti-viral activity and exerts lower toxicity. We show that Saq-NO inhibited the generation of various cytokines in ConA-stimulated unfractionated murine spleen cells and rat lymph nodes stimulated with ConA as well as in purified CD4(+) T cells in vitro and reduced the circulating levels of cytokines in mice challenged with anti-CD3 antibody. Furthermore, Saq-NO reduced IL-17 and IFN-gamma production in myelin basic protein (MBP)-specific cells isolated from rats immunized with MBP. These findings translated well into the in vivo setting as Saq-NO ameliorated the course of the disease in two preclinical models of multiple sclerosis. Our results demonstrate that Saq-NO exerts immunomodulatory effects that warrant studies on its application in autoimmune diseases.
Кључне речи:
T cell / Multiple sclerosis / EAE / Cytokines / AutoimmunityИзвор:
Journal of Neuroimmunology, 2013, 259, 1-2, 55-65Издавач:
- Elsevier Science Bv, Amsterdam
Финансирање / пројекти:
- Ћелијски и молекулски механизми опоравка пацова од експерименталног аутоимунског енцефаломијелитиса (RS-MESTD-Basic Research (BR or ON)-173035)
- Молекуларни механизми физиолошке и фармаколошке контроле инфламације и канцера (RS-MESTD-Basic Research (BR or ON)-173013)
DOI: 10.1016/j.jneuroim.2013.03.010
ISSN: 0165-5728
PubMed: 23602714
WoS: 000319848100007
Scopus: 2-s2.0-84877600866
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Petković, Filip AU - Blazevski, Jana AU - Momcilović, Miljana AU - Timotijević, Gordana AU - Zocca, Mai-Britt AU - Mijatović, Sanja AU - Maksimović-Ivanić, Danijela AU - Mangano, Katia AU - Fagone, Paolo AU - Stošić-Grujičić, Stanislava AU - Nicoletti, Ferdinando AU - Miljković, Djordje PY - 2013 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/647 AB - NO-hybridization of the HIV protease inhibitor Saquinavir generates a new chemical entity named Saq-NO, that retains the anti-viral activity and exerts lower toxicity. We show that Saq-NO inhibited the generation of various cytokines in ConA-stimulated unfractionated murine spleen cells and rat lymph nodes stimulated with ConA as well as in purified CD4(+) T cells in vitro and reduced the circulating levels of cytokines in mice challenged with anti-CD3 antibody. Furthermore, Saq-NO reduced IL-17 and IFN-gamma production in myelin basic protein (MBP)-specific cells isolated from rats immunized with MBP. These findings translated well into the in vivo setting as Saq-NO ameliorated the course of the disease in two preclinical models of multiple sclerosis. Our results demonstrate that Saq-NO exerts immunomodulatory effects that warrant studies on its application in autoimmune diseases. PB - Elsevier Science Bv, Amsterdam T2 - Journal of Neuroimmunology T1 - Saquinavir-NO inhibits S6 kinase activity, impairs secretion of the encephalytogenic cytokines interleukin-17 and interferon-gamma and ameliorates experimental autoimmune encephalomyelitis EP - 65 IS - 1-2 SP - 55 VL - 259 DO - 10.1016/j.jneuroim.2013.03.010 ER -
@article{ author = "Petković, Filip and Blazevski, Jana and Momcilović, Miljana and Timotijević, Gordana and Zocca, Mai-Britt and Mijatović, Sanja and Maksimović-Ivanić, Danijela and Mangano, Katia and Fagone, Paolo and Stošić-Grujičić, Stanislava and Nicoletti, Ferdinando and Miljković, Djordje", year = "2013", abstract = "NO-hybridization of the HIV protease inhibitor Saquinavir generates a new chemical entity named Saq-NO, that retains the anti-viral activity and exerts lower toxicity. We show that Saq-NO inhibited the generation of various cytokines in ConA-stimulated unfractionated murine spleen cells and rat lymph nodes stimulated with ConA as well as in purified CD4(+) T cells in vitro and reduced the circulating levels of cytokines in mice challenged with anti-CD3 antibody. Furthermore, Saq-NO reduced IL-17 and IFN-gamma production in myelin basic protein (MBP)-specific cells isolated from rats immunized with MBP. These findings translated well into the in vivo setting as Saq-NO ameliorated the course of the disease in two preclinical models of multiple sclerosis. Our results demonstrate that Saq-NO exerts immunomodulatory effects that warrant studies on its application in autoimmune diseases.", publisher = "Elsevier Science Bv, Amsterdam", journal = "Journal of Neuroimmunology", title = "Saquinavir-NO inhibits S6 kinase activity, impairs secretion of the encephalytogenic cytokines interleukin-17 and interferon-gamma and ameliorates experimental autoimmune encephalomyelitis", pages = "65-55", number = "1-2", volume = "259", doi = "10.1016/j.jneuroim.2013.03.010" }
Petković, F., Blazevski, J., Momcilović, M., Timotijević, G., Zocca, M., Mijatović, S., Maksimović-Ivanić, D., Mangano, K., Fagone, P., Stošić-Grujičić, S., Nicoletti, F.,& Miljković, D.. (2013). Saquinavir-NO inhibits S6 kinase activity, impairs secretion of the encephalytogenic cytokines interleukin-17 and interferon-gamma and ameliorates experimental autoimmune encephalomyelitis. in Journal of Neuroimmunology Elsevier Science Bv, Amsterdam., 259(1-2), 55-65. https://doi.org/10.1016/j.jneuroim.2013.03.010
Petković F, Blazevski J, Momcilović M, Timotijević G, Zocca M, Mijatović S, Maksimović-Ivanić D, Mangano K, Fagone P, Stošić-Grujičić S, Nicoletti F, Miljković D. Saquinavir-NO inhibits S6 kinase activity, impairs secretion of the encephalytogenic cytokines interleukin-17 and interferon-gamma and ameliorates experimental autoimmune encephalomyelitis. in Journal of Neuroimmunology. 2013;259(1-2):55-65. doi:10.1016/j.jneuroim.2013.03.010 .
Petković, Filip, Blazevski, Jana, Momcilović, Miljana, Timotijević, Gordana, Zocca, Mai-Britt, Mijatović, Sanja, Maksimović-Ivanić, Danijela, Mangano, Katia, Fagone, Paolo, Stošić-Grujičić, Stanislava, Nicoletti, Ferdinando, Miljković, Djordje, "Saquinavir-NO inhibits S6 kinase activity, impairs secretion of the encephalytogenic cytokines interleukin-17 and interferon-gamma and ameliorates experimental autoimmune encephalomyelitis" in Journal of Neuroimmunology, 259, no. 1-2 (2013):55-65, https://doi.org/10.1016/j.jneuroim.2013.03.010 . .