Brh2 and Rad51 promote telomere maintenance in Ustilago maydis, a new model system of DNA repair proteins at telomeres
Abstract
Recent studies implicate a number of DNA repair proteins in mammalian telomere maintenance. However, because several key repair proteins in mammals are missing from the well-studied budding and fission yeast, their roles at telomeres cannot be modeled in standard fungi. In this report, we explored the dimorphic fungus Ustilago maydis as an alternative model for telomere research. This fungus, which belongs to the phylum Basidiomycota, has a telomere repeat unit that is identical to the mammalian repeat, as well as a constellation of DNA repair proteins that more closely mimic the mammalian collection. We showed that the two core components of homology-directed repair (HDR) in U. maydis, namely Brh2 and Rad51, both promote telomere maintenance in telomerase positive cells, just like in mammals. In addition, we found that Brh2 is localized to telomeres in vivo, suggesting that it acts directly at chromosome ends. We surveyed a series of mutants with DNA repair defects, and found many of ...them to have short telomeres. Our results indicate that factors involved in DNA repair are probably also needed for optimal telomere maintenance in U. maydis, and that this fungus is a useful alternative model system for telomere research.
Keywords:
Ustilago maydis / Telomere / Rad51 / Homology-directed repair / Brh2Source:
DNA Repair, 2013, 12, 7, 472-479Publisher:
- Elsevier Science Bv, Amsterdam
Funding / projects:
- Pilot Project Grant from the Weill Cornell Cancer Center
- NIH [GM042482]
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM062631, R01GM042482] Funding Source: NIH RePORTER
Note:
- Peer-reviewed manuscript: https://imagine.imgge.bg.ac.rs/handle/123456789/1622
Related info:
DOI: 10.1016/j.dnarep.2013.04.027
ISSN: 1568-7864
PubMed: 23726221
WoS: 000321417300003
Scopus: 2-s2.0-84878648069
Collections
Institution/Community
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Yu, Eun Young AU - Kojić, Milorad AU - Holloman, William K. AU - Lue, Neal F. PY - 2013 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/653 AB - Recent studies implicate a number of DNA repair proteins in mammalian telomere maintenance. However, because several key repair proteins in mammals are missing from the well-studied budding and fission yeast, their roles at telomeres cannot be modeled in standard fungi. In this report, we explored the dimorphic fungus Ustilago maydis as an alternative model for telomere research. This fungus, which belongs to the phylum Basidiomycota, has a telomere repeat unit that is identical to the mammalian repeat, as well as a constellation of DNA repair proteins that more closely mimic the mammalian collection. We showed that the two core components of homology-directed repair (HDR) in U. maydis, namely Brh2 and Rad51, both promote telomere maintenance in telomerase positive cells, just like in mammals. In addition, we found that Brh2 is localized to telomeres in vivo, suggesting that it acts directly at chromosome ends. We surveyed a series of mutants with DNA repair defects, and found many of them to have short telomeres. Our results indicate that factors involved in DNA repair are probably also needed for optimal telomere maintenance in U. maydis, and that this fungus is a useful alternative model system for telomere research. PB - Elsevier Science Bv, Amsterdam T2 - DNA Repair T1 - Brh2 and Rad51 promote telomere maintenance in Ustilago maydis, a new model system of DNA repair proteins at telomeres EP - 479 IS - 7 SP - 472 VL - 12 DO - 10.1016/j.dnarep.2013.04.027 ER -
@article{ author = "Yu, Eun Young and Kojić, Milorad and Holloman, William K. and Lue, Neal F.", year = "2013", abstract = "Recent studies implicate a number of DNA repair proteins in mammalian telomere maintenance. However, because several key repair proteins in mammals are missing from the well-studied budding and fission yeast, their roles at telomeres cannot be modeled in standard fungi. In this report, we explored the dimorphic fungus Ustilago maydis as an alternative model for telomere research. This fungus, which belongs to the phylum Basidiomycota, has a telomere repeat unit that is identical to the mammalian repeat, as well as a constellation of DNA repair proteins that more closely mimic the mammalian collection. We showed that the two core components of homology-directed repair (HDR) in U. maydis, namely Brh2 and Rad51, both promote telomere maintenance in telomerase positive cells, just like in mammals. In addition, we found that Brh2 is localized to telomeres in vivo, suggesting that it acts directly at chromosome ends. We surveyed a series of mutants with DNA repair defects, and found many of them to have short telomeres. Our results indicate that factors involved in DNA repair are probably also needed for optimal telomere maintenance in U. maydis, and that this fungus is a useful alternative model system for telomere research.", publisher = "Elsevier Science Bv, Amsterdam", journal = "DNA Repair", title = "Brh2 and Rad51 promote telomere maintenance in Ustilago maydis, a new model system of DNA repair proteins at telomeres", pages = "479-472", number = "7", volume = "12", doi = "10.1016/j.dnarep.2013.04.027" }
Yu, E. Y., Kojić, M., Holloman, W. K.,& Lue, N. F.. (2013). Brh2 and Rad51 promote telomere maintenance in Ustilago maydis, a new model system of DNA repair proteins at telomeres. in DNA Repair Elsevier Science Bv, Amsterdam., 12(7), 472-479. https://doi.org/10.1016/j.dnarep.2013.04.027
Yu EY, Kojić M, Holloman WK, Lue NF. Brh2 and Rad51 promote telomere maintenance in Ustilago maydis, a new model system of DNA repair proteins at telomeres. in DNA Repair. 2013;12(7):472-479. doi:10.1016/j.dnarep.2013.04.027 .
Yu, Eun Young, Kojić, Milorad, Holloman, William K., Lue, Neal F., "Brh2 and Rad51 promote telomere maintenance in Ustilago maydis, a new model system of DNA repair proteins at telomeres" in DNA Repair, 12, no. 7 (2013):472-479, https://doi.org/10.1016/j.dnarep.2013.04.027 . .