CXCL12-gamma expression is inhibited in neuroinflammation
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2013
Authors
Timotijević, GordanaPetković, Filip
Blazevski, Jana
Momcilović, Miljana
Mostarica-Stojković, Marija
Miljković, Djordje
Article (Published version)
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Show full item recordAbstract
CXCL12 plays a protective role in CNS autoimmunity. Expression of CXCL12-gamma, which has distinct structural and functional properties than the other isoforms of CXCL12, was determined in spinal cords of rats immunized to develop experimental autoimmune encephalomyelitis (EAE). CNS expression of CXCL12-gamma was markedly lower in EAE-prone Dark Agouti rats than in EAE-resistant Albino Oxford rats, both in spinal cord homogenates and micro-blood vessels isolated from spinal cords. Inhibition of nitric oxide (NO) synthesis in DA rats upregulated, while donation of NO in AO rats downregulated CNS expression of CXCL12-gamma. NO inhibited CXCL12-gamma expression in astrocytes in vitro. A splice variant of CXCL12-gamma which migrates into nucleolus was not detected in spinal cord or astrocytes. Thus, CXCL12-gamma is expressed in the CNS after EAE induction, but its expression is markedly suppressed in spinal cord affected with full blown inflammation. NO is an important regulator of CXCL12-...gamma expression in neuroinflammation.
Keywords:
Nitric oxide / Micro-blood vessel / Experimental autoimmune / encephalomyelitis / CXCL12 / AstrocyteSource:
Brain Research, 2013, 1519, 120-126Publisher:
- Elsevier Science Bv, Amsterdam
Funding / projects:
- Cellular and molecular mechanisms of recovery of rats from experimental autoimmune encephalomyelitis (RS-MESTD-Basic Research (BR or ON)-173035)
- Immunopathogenic and regulatory mechanisms in autoimmune diseases and chronic inflamation (RS-MESTD-Basic Research (BR or ON)-175038)
- Molecular mechanisms of physiological and pharmacological control of inflammation and cancer (RS-MESTD-Basic Research (BR or ON)-173013)
DOI: 10.1016/j.brainres.2013.04.056
ISSN: 0006-8993
PubMed: 23651977
WoS: 000321473500014
Scopus: 2-s2.0-84878749945
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Institution/Community
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Timotijević, Gordana AU - Petković, Filip AU - Blazevski, Jana AU - Momcilović, Miljana AU - Mostarica-Stojković, Marija AU - Miljković, Djordje PY - 2013 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/654 AB - CXCL12 plays a protective role in CNS autoimmunity. Expression of CXCL12-gamma, which has distinct structural and functional properties than the other isoforms of CXCL12, was determined in spinal cords of rats immunized to develop experimental autoimmune encephalomyelitis (EAE). CNS expression of CXCL12-gamma was markedly lower in EAE-prone Dark Agouti rats than in EAE-resistant Albino Oxford rats, both in spinal cord homogenates and micro-blood vessels isolated from spinal cords. Inhibition of nitric oxide (NO) synthesis in DA rats upregulated, while donation of NO in AO rats downregulated CNS expression of CXCL12-gamma. NO inhibited CXCL12-gamma expression in astrocytes in vitro. A splice variant of CXCL12-gamma which migrates into nucleolus was not detected in spinal cord or astrocytes. Thus, CXCL12-gamma is expressed in the CNS after EAE induction, but its expression is markedly suppressed in spinal cord affected with full blown inflammation. NO is an important regulator of CXCL12-gamma expression in neuroinflammation. PB - Elsevier Science Bv, Amsterdam T2 - Brain Research T1 - CXCL12-gamma expression is inhibited in neuroinflammation EP - 126 SP - 120 VL - 1519 DO - 10.1016/j.brainres.2013.04.056 ER -
@article{ author = "Timotijević, Gordana and Petković, Filip and Blazevski, Jana and Momcilović, Miljana and Mostarica-Stojković, Marija and Miljković, Djordje", year = "2013", abstract = "CXCL12 plays a protective role in CNS autoimmunity. Expression of CXCL12-gamma, which has distinct structural and functional properties than the other isoforms of CXCL12, was determined in spinal cords of rats immunized to develop experimental autoimmune encephalomyelitis (EAE). CNS expression of CXCL12-gamma was markedly lower in EAE-prone Dark Agouti rats than in EAE-resistant Albino Oxford rats, both in spinal cord homogenates and micro-blood vessels isolated from spinal cords. Inhibition of nitric oxide (NO) synthesis in DA rats upregulated, while donation of NO in AO rats downregulated CNS expression of CXCL12-gamma. NO inhibited CXCL12-gamma expression in astrocytes in vitro. A splice variant of CXCL12-gamma which migrates into nucleolus was not detected in spinal cord or astrocytes. Thus, CXCL12-gamma is expressed in the CNS after EAE induction, but its expression is markedly suppressed in spinal cord affected with full blown inflammation. NO is an important regulator of CXCL12-gamma expression in neuroinflammation.", publisher = "Elsevier Science Bv, Amsterdam", journal = "Brain Research", title = "CXCL12-gamma expression is inhibited in neuroinflammation", pages = "126-120", volume = "1519", doi = "10.1016/j.brainres.2013.04.056" }
Timotijević, G., Petković, F., Blazevski, J., Momcilović, M., Mostarica-Stojković, M.,& Miljković, D.. (2013). CXCL12-gamma expression is inhibited in neuroinflammation. in Brain Research Elsevier Science Bv, Amsterdam., 1519, 120-126. https://doi.org/10.1016/j.brainres.2013.04.056
Timotijević G, Petković F, Blazevski J, Momcilović M, Mostarica-Stojković M, Miljković D. CXCL12-gamma expression is inhibited in neuroinflammation. in Brain Research. 2013;1519:120-126. doi:10.1016/j.brainres.2013.04.056 .
Timotijević, Gordana, Petković, Filip, Blazevski, Jana, Momcilović, Miljana, Mostarica-Stojković, Marija, Miljković, Djordje, "CXCL12-gamma expression is inhibited in neuroinflammation" in Brain Research, 1519 (2013):120-126, https://doi.org/10.1016/j.brainres.2013.04.056 . .