Dynamics and stoichiometry of a regulated enhancer-binding protein in live Escherichia coli cells
2013
Аутори
Mehta, ParulJovanović, Goran
Lenn, Tchern
Bruckbauer, Andreas
Engl, Christoph
Ying, Liming
Buck, Martin
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Bacterial enhancer-dependent transcription systems support major adaptive responses and offer a singular paradigm in gene control analogous to complex eukaryotic systems. Here we report new mechanistic insights into the control of one-membrane stress-responsive bacterial enhancer-dependent system. Using millisecond single-molecule fluorescence microscopy of live cells we determine the localizations, two-dimensional diffusion dynamics and stoichiometries of complexes of the bacterial enhancer-binding ATPase PspF during its action at promoters as regulated by inner membrane interacting negative controller PspA. We establish that a stable repressive PspF-PspA complex is located in the nucleoid, transiently communicating with the inner membrane via PspA. The PspF as a hexamer stably binds only one of the two psp promoters at a time, suggesting that psp promoters will fire asynchronously and cooperative interactions of PspF with the basal transcription complex influence dynamics of the PspF... hexamer-DNA complex and regulation of the psp promoters.
Извор:
Nature Communications, 2013, 4Издавач:
- Nature Publishing Group, London
Финансирање / пројекти:
- BBSRC
- Cancer Research UK
- BBSRC [BB/G00594X/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/G00594X/1, JF20607/2] Funding Source: researchfish
DOI: 10.1038/ncomms2997
ISSN: 2041-1723
PubMed: 23764692
WoS: 000323624600020
Scopus: 2-s2.0-84879645709
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Mehta, Parul AU - Jovanović, Goran AU - Lenn, Tchern AU - Bruckbauer, Andreas AU - Engl, Christoph AU - Ying, Liming AU - Buck, Martin PY - 2013 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/660 AB - Bacterial enhancer-dependent transcription systems support major adaptive responses and offer a singular paradigm in gene control analogous to complex eukaryotic systems. Here we report new mechanistic insights into the control of one-membrane stress-responsive bacterial enhancer-dependent system. Using millisecond single-molecule fluorescence microscopy of live cells we determine the localizations, two-dimensional diffusion dynamics and stoichiometries of complexes of the bacterial enhancer-binding ATPase PspF during its action at promoters as regulated by inner membrane interacting negative controller PspA. We establish that a stable repressive PspF-PspA complex is located in the nucleoid, transiently communicating with the inner membrane via PspA. The PspF as a hexamer stably binds only one of the two psp promoters at a time, suggesting that psp promoters will fire asynchronously and cooperative interactions of PspF with the basal transcription complex influence dynamics of the PspF hexamer-DNA complex and regulation of the psp promoters. PB - Nature Publishing Group, London T2 - Nature Communications T1 - Dynamics and stoichiometry of a regulated enhancer-binding protein in live Escherichia coli cells VL - 4 DO - 10.1038/ncomms2997 ER -
@article{ author = "Mehta, Parul and Jovanović, Goran and Lenn, Tchern and Bruckbauer, Andreas and Engl, Christoph and Ying, Liming and Buck, Martin", year = "2013", abstract = "Bacterial enhancer-dependent transcription systems support major adaptive responses and offer a singular paradigm in gene control analogous to complex eukaryotic systems. Here we report new mechanistic insights into the control of one-membrane stress-responsive bacterial enhancer-dependent system. Using millisecond single-molecule fluorescence microscopy of live cells we determine the localizations, two-dimensional diffusion dynamics and stoichiometries of complexes of the bacterial enhancer-binding ATPase PspF during its action at promoters as regulated by inner membrane interacting negative controller PspA. We establish that a stable repressive PspF-PspA complex is located in the nucleoid, transiently communicating with the inner membrane via PspA. The PspF as a hexamer stably binds only one of the two psp promoters at a time, suggesting that psp promoters will fire asynchronously and cooperative interactions of PspF with the basal transcription complex influence dynamics of the PspF hexamer-DNA complex and regulation of the psp promoters.", publisher = "Nature Publishing Group, London", journal = "Nature Communications", title = "Dynamics and stoichiometry of a regulated enhancer-binding protein in live Escherichia coli cells", volume = "4", doi = "10.1038/ncomms2997" }
Mehta, P., Jovanović, G., Lenn, T., Bruckbauer, A., Engl, C., Ying, L.,& Buck, M.. (2013). Dynamics and stoichiometry of a regulated enhancer-binding protein in live Escherichia coli cells. in Nature Communications Nature Publishing Group, London., 4. https://doi.org/10.1038/ncomms2997
Mehta P, Jovanović G, Lenn T, Bruckbauer A, Engl C, Ying L, Buck M. Dynamics and stoichiometry of a regulated enhancer-binding protein in live Escherichia coli cells. in Nature Communications. 2013;4. doi:10.1038/ncomms2997 .
Mehta, Parul, Jovanović, Goran, Lenn, Tchern, Bruckbauer, Andreas, Engl, Christoph, Ying, Liming, Buck, Martin, "Dynamics and stoichiometry of a regulated enhancer-binding protein in live Escherichia coli cells" in Nature Communications, 4 (2013), https://doi.org/10.1038/ncomms2997 . .