Construction and functional analysis of novel dominant-negative mutant of human SOX18 protein
Само за регистроване кориснике
2013
Аутори
Milivojević, MilenaPetrović, Isidora
Kovačević Grujičić, Nataša
Popović, Jelena
Mojsin, Marija
Stevanović, Milena
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
SOX18 transcription factor plays important roles in a range of biological processes such as vasculogenesis, hair follicle development, lymphangiogenesis, atherosclerosis, and angiogenesis. In this paper we present the generation of a novel SOX18 dominant-negative mutant (SOX18DN) encoding truncated SOX18 protein that lacks a trans-activation domain. We show that both wild-type SOX18 (SOX18wt) and truncated human SOX18 proteins are able to bind to their consensus sequence in vitro. Functional analysis confirmed that SOX18wt has potent trans-activation properties, while SOX18DN displays dominant-negative effect. We believe that these SOX18wt and SOX18DN expression constructs could be successfully used for further characterization of the function of this protein.
Кључне речи:
truncated protein / transcription factor / SOX18 / dominant-negativeИзвор:
Biochemistry-Moscow, 2013, 78, 11, 1287-1292Издавач:
- Maik Nauka/Interperiodica/Springer, New York
Финансирање / пројекти:
- Проучавање сигналних путева и епигенетичких механизама укључених у контролу експресије хуманих SOX гена: даље расветљавање њихове улоге у одређивању судбине и диференцијацији ћелија (RS-MESTD-Basic Research (BR or ON)-173051)
DOI: 10.1134/S0006297913110096
ISSN: 0006-2979
PubMed: 24460943
WoS: 000327070900009
Scopus: 2-s2.0-84889028951
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Milivojević, Milena AU - Petrović, Isidora AU - Kovačević Grujičić, Nataša AU - Popović, Jelena AU - Mojsin, Marija AU - Stevanović, Milena PY - 2013 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/672 AB - SOX18 transcription factor plays important roles in a range of biological processes such as vasculogenesis, hair follicle development, lymphangiogenesis, atherosclerosis, and angiogenesis. In this paper we present the generation of a novel SOX18 dominant-negative mutant (SOX18DN) encoding truncated SOX18 protein that lacks a trans-activation domain. We show that both wild-type SOX18 (SOX18wt) and truncated human SOX18 proteins are able to bind to their consensus sequence in vitro. Functional analysis confirmed that SOX18wt has potent trans-activation properties, while SOX18DN displays dominant-negative effect. We believe that these SOX18wt and SOX18DN expression constructs could be successfully used for further characterization of the function of this protein. PB - Maik Nauka/Interperiodica/Springer, New York T2 - Biochemistry-Moscow T1 - Construction and functional analysis of novel dominant-negative mutant of human SOX18 protein EP - 1292 IS - 11 SP - 1287 VL - 78 DO - 10.1134/S0006297913110096 ER -
@article{ author = "Milivojević, Milena and Petrović, Isidora and Kovačević Grujičić, Nataša and Popović, Jelena and Mojsin, Marija and Stevanović, Milena", year = "2013", abstract = "SOX18 transcription factor plays important roles in a range of biological processes such as vasculogenesis, hair follicle development, lymphangiogenesis, atherosclerosis, and angiogenesis. In this paper we present the generation of a novel SOX18 dominant-negative mutant (SOX18DN) encoding truncated SOX18 protein that lacks a trans-activation domain. We show that both wild-type SOX18 (SOX18wt) and truncated human SOX18 proteins are able to bind to their consensus sequence in vitro. Functional analysis confirmed that SOX18wt has potent trans-activation properties, while SOX18DN displays dominant-negative effect. We believe that these SOX18wt and SOX18DN expression constructs could be successfully used for further characterization of the function of this protein.", publisher = "Maik Nauka/Interperiodica/Springer, New York", journal = "Biochemistry-Moscow", title = "Construction and functional analysis of novel dominant-negative mutant of human SOX18 protein", pages = "1292-1287", number = "11", volume = "78", doi = "10.1134/S0006297913110096" }
Milivojević, M., Petrović, I., Kovačević Grujičić, N., Popović, J., Mojsin, M.,& Stevanović, M.. (2013). Construction and functional analysis of novel dominant-negative mutant of human SOX18 protein. in Biochemistry-Moscow Maik Nauka/Interperiodica/Springer, New York., 78(11), 1287-1292. https://doi.org/10.1134/S0006297913110096
Milivojević M, Petrović I, Kovačević Grujičić N, Popović J, Mojsin M, Stevanović M. Construction and functional analysis of novel dominant-negative mutant of human SOX18 protein. in Biochemistry-Moscow. 2013;78(11):1287-1292. doi:10.1134/S0006297913110096 .
Milivojević, Milena, Petrović, Isidora, Kovačević Grujičić, Nataša, Popović, Jelena, Mojsin, Marija, Stevanović, Milena, "Construction and functional analysis of novel dominant-negative mutant of human SOX18 protein" in Biochemistry-Moscow, 78, no. 11 (2013):1287-1292, https://doi.org/10.1134/S0006297913110096 . .