Quercetin and lithium chloride modulate Wnt signaling in pluripotent embryonal carcinoma NT2/D1 cells
Apstrakt
Wnt signaling functions in numerous cellular activities such as cell fate determination, patterning, and migration in embryogenesis, apoptosis, etc. In this study, we used quercetin and lithium chloride to investigate modulations of the Wnt signaling pathway in human pluripotent embryonal carcinoma NT2/D1 cell line. First, we optimized conditions for NT2/D1 cell treatments with quercetin and lithium chloride and assessed their cytotoxic effects on the cells, cell viability and proliferation rate. Our results showed that induction of cell death by quercetin and LiCl is p53-dependent in NT2/D cells. We also examined the degree of Wnt signaling modulations by analyzing the expression of c-myc, a wellknown Wnt signaling target gene. Since the retinoic acid induction of NT2/D1 cells is good in an in vitro model system for human neural differentiation, studying Wnt signaling modulation in NT2/D1 would contribute to a better understanding of the mechanisms involved in neural stem cell mainten...ance and human neural development.
Ključne reči:
Wnt signaling / quercetin / NT2/D1 cells / LiClIzvor:
Archives of Biological Sciences, 2013, 65, 1, 201-209Izdavač:
- Srpsko biološko društvo, Beograd, i dr.
Finansiranje / projekti:
- Proučavanje signalnih puteva i epigenetičkih mehanizama uključenih u kontrolu ekspresije humanih SOX gena: dalje rasvetljavanje njihove uloge u određivanju sudbine i diferencijaciji ćelija (RS-MESTD-Basic Research (BR or ON)-173051)
Povezane informacije:
- Povezani sadržaj
doi.org/10.2298/ABS1301201M
DOI: 10.2298/ABS1301201M
ISSN: 0354-4664
WoS: 000312046300025
Scopus: 2-s2.0-84873114320
Institucija/grupa
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Mojsin, Marija AU - Topalović, Vladanka AU - Marjanović, Jelena AU - Stevanović, Milena PY - 2013 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/682 AB - Wnt signaling functions in numerous cellular activities such as cell fate determination, patterning, and migration in embryogenesis, apoptosis, etc. In this study, we used quercetin and lithium chloride to investigate modulations of the Wnt signaling pathway in human pluripotent embryonal carcinoma NT2/D1 cell line. First, we optimized conditions for NT2/D1 cell treatments with quercetin and lithium chloride and assessed their cytotoxic effects on the cells, cell viability and proliferation rate. Our results showed that induction of cell death by quercetin and LiCl is p53-dependent in NT2/D cells. We also examined the degree of Wnt signaling modulations by analyzing the expression of c-myc, a wellknown Wnt signaling target gene. Since the retinoic acid induction of NT2/D1 cells is good in an in vitro model system for human neural differentiation, studying Wnt signaling modulation in NT2/D1 would contribute to a better understanding of the mechanisms involved in neural stem cell maintenance and human neural development. PB - Srpsko biološko društvo, Beograd, i dr. T2 - Archives of Biological Sciences T1 - Quercetin and lithium chloride modulate Wnt signaling in pluripotent embryonal carcinoma NT2/D1 cells EP - 209 IS - 1 SP - 201 VL - 65 DO - 10.2298/ABS1301201M ER -
@article{ author = "Mojsin, Marija and Topalović, Vladanka and Marjanović, Jelena and Stevanović, Milena", year = "2013", abstract = "Wnt signaling functions in numerous cellular activities such as cell fate determination, patterning, and migration in embryogenesis, apoptosis, etc. In this study, we used quercetin and lithium chloride to investigate modulations of the Wnt signaling pathway in human pluripotent embryonal carcinoma NT2/D1 cell line. First, we optimized conditions for NT2/D1 cell treatments with quercetin and lithium chloride and assessed their cytotoxic effects on the cells, cell viability and proliferation rate. Our results showed that induction of cell death by quercetin and LiCl is p53-dependent in NT2/D cells. We also examined the degree of Wnt signaling modulations by analyzing the expression of c-myc, a wellknown Wnt signaling target gene. Since the retinoic acid induction of NT2/D1 cells is good in an in vitro model system for human neural differentiation, studying Wnt signaling modulation in NT2/D1 would contribute to a better understanding of the mechanisms involved in neural stem cell maintenance and human neural development.", publisher = "Srpsko biološko društvo, Beograd, i dr.", journal = "Archives of Biological Sciences", title = "Quercetin and lithium chloride modulate Wnt signaling in pluripotent embryonal carcinoma NT2/D1 cells", pages = "209-201", number = "1", volume = "65", doi = "10.2298/ABS1301201M" }
Mojsin, M., Topalović, V., Marjanović, J.,& Stevanović, M.. (2013). Quercetin and lithium chloride modulate Wnt signaling in pluripotent embryonal carcinoma NT2/D1 cells. in Archives of Biological Sciences Srpsko biološko društvo, Beograd, i dr.., 65(1), 201-209. https://doi.org/10.2298/ABS1301201M
Mojsin M, Topalović V, Marjanović J, Stevanović M. Quercetin and lithium chloride modulate Wnt signaling in pluripotent embryonal carcinoma NT2/D1 cells. in Archives of Biological Sciences. 2013;65(1):201-209. doi:10.2298/ABS1301201M .
Mojsin, Marija, Topalović, Vladanka, Marjanović, Jelena, Stevanović, Milena, "Quercetin and lithium chloride modulate Wnt signaling in pluripotent embryonal carcinoma NT2/D1 cells" in Archives of Biological Sciences, 65, no. 1 (2013):201-209, https://doi.org/10.2298/ABS1301201M . .