Differential response of three contrasting pea (Pisum arvense, P. Sativum and P. Fulvum) species to salt stress: Assessment of variation in antioxidative defence and miRNA expression
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Autori
Miljuš-Đukić, JovankaStanisavljević, Nemanja
Radović, S.
Jovanović, Živko
Mikić, A.
Maksimović, Vesna R.
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Soil salinity is one of the major abiotic stresses leading to crops yield failure. For investigation of salinity response in pea, in vitro cultures of three pea species (Pisum arvense, P. sativum and P. fulvum) were induced. Regenerated shoots of these pea species were grown on a medium with moderate and high NaCl concentrations (75, 120, 150 and 200 mM NaCl) to compare the antioxidative capacity in response to salt stress. Growth reduction was observed in all species and it correlated with the increase of NaCl concentration. After four weeks of treatment, the crude tissue extracts were used for measuring the stress response parameters. Level of lipid peroxidation increased in all three species, but in P. fulvum the effect of NaCl was less pronounced. Total chlorophyll and phenolic content showed differences among the three species, while the DPPH-scavenging activity was the most evident in P. fulvum. The northern blot analysis of the miRNA398 expression showed a similar pattern betwee...n P. sativum and P. arvense but different to that of P. fulvum, as in P. fulvum miRNA 398 was only expressed at moderate NaCl concentration, with total inhibition at high concentrations. On the basis of these results it can be concluded that P. fulvum could represent a gene pool for improving P. sativum and P. arvense stress defence capacity.
Ključne reči:
Salt stress / Pea / Nacl / MiRNA398a. / MDA / FC assay / DPPH assayIzvor:
Australian Journal of Crop Science, 2013, 7, 13, 2145-2153Institucija/grupa
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Miljuš-Đukić, Jovanka AU - Stanisavljević, Nemanja AU - Radović, S. AU - Jovanović, Živko AU - Mikić, A. AU - Maksimović, Vesna R. PY - 2013 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/688 AB - Soil salinity is one of the major abiotic stresses leading to crops yield failure. For investigation of salinity response in pea, in vitro cultures of three pea species (Pisum arvense, P. sativum and P. fulvum) were induced. Regenerated shoots of these pea species were grown on a medium with moderate and high NaCl concentrations (75, 120, 150 and 200 mM NaCl) to compare the antioxidative capacity in response to salt stress. Growth reduction was observed in all species and it correlated with the increase of NaCl concentration. After four weeks of treatment, the crude tissue extracts were used for measuring the stress response parameters. Level of lipid peroxidation increased in all three species, but in P. fulvum the effect of NaCl was less pronounced. Total chlorophyll and phenolic content showed differences among the three species, while the DPPH-scavenging activity was the most evident in P. fulvum. The northern blot analysis of the miRNA398 expression showed a similar pattern between P. sativum and P. arvense but different to that of P. fulvum, as in P. fulvum miRNA 398 was only expressed at moderate NaCl concentration, with total inhibition at high concentrations. On the basis of these results it can be concluded that P. fulvum could represent a gene pool for improving P. sativum and P. arvense stress defence capacity. T2 - Australian Journal of Crop Science T1 - Differential response of three contrasting pea (Pisum arvense, P. Sativum and P. Fulvum) species to salt stress: Assessment of variation in antioxidative defence and miRNA expression EP - 2153 IS - 13 SP - 2145 VL - 7 UR - https://hdl.handle.net/21.15107/rcub_imagine_688 ER -
@article{ author = "Miljuš-Đukić, Jovanka and Stanisavljević, Nemanja and Radović, S. and Jovanović, Živko and Mikić, A. and Maksimović, Vesna R.", year = "2013", abstract = "Soil salinity is one of the major abiotic stresses leading to crops yield failure. For investigation of salinity response in pea, in vitro cultures of three pea species (Pisum arvense, P. sativum and P. fulvum) were induced. Regenerated shoots of these pea species were grown on a medium with moderate and high NaCl concentrations (75, 120, 150 and 200 mM NaCl) to compare the antioxidative capacity in response to salt stress. Growth reduction was observed in all species and it correlated with the increase of NaCl concentration. After four weeks of treatment, the crude tissue extracts were used for measuring the stress response parameters. Level of lipid peroxidation increased in all three species, but in P. fulvum the effect of NaCl was less pronounced. Total chlorophyll and phenolic content showed differences among the three species, while the DPPH-scavenging activity was the most evident in P. fulvum. The northern blot analysis of the miRNA398 expression showed a similar pattern between P. sativum and P. arvense but different to that of P. fulvum, as in P. fulvum miRNA 398 was only expressed at moderate NaCl concentration, with total inhibition at high concentrations. On the basis of these results it can be concluded that P. fulvum could represent a gene pool for improving P. sativum and P. arvense stress defence capacity.", journal = "Australian Journal of Crop Science", title = "Differential response of three contrasting pea (Pisum arvense, P. Sativum and P. Fulvum) species to salt stress: Assessment of variation in antioxidative defence and miRNA expression", pages = "2153-2145", number = "13", volume = "7", url = "https://hdl.handle.net/21.15107/rcub_imagine_688" }
Miljuš-Đukić, J., Stanisavljević, N., Radović, S., Jovanović, Ž., Mikić, A.,& Maksimović, V. R.. (2013). Differential response of three contrasting pea (Pisum arvense, P. Sativum and P. Fulvum) species to salt stress: Assessment of variation in antioxidative defence and miRNA expression. in Australian Journal of Crop Science, 7(13), 2145-2153. https://hdl.handle.net/21.15107/rcub_imagine_688
Miljuš-Đukić J, Stanisavljević N, Radović S, Jovanović Ž, Mikić A, Maksimović VR. Differential response of three contrasting pea (Pisum arvense, P. Sativum and P. Fulvum) species to salt stress: Assessment of variation in antioxidative defence and miRNA expression. in Australian Journal of Crop Science. 2013;7(13):2145-2153. https://hdl.handle.net/21.15107/rcub_imagine_688 .
Miljuš-Đukić, Jovanka, Stanisavljević, Nemanja, Radović, S., Jovanović, Živko, Mikić, A., Maksimović, Vesna R., "Differential response of three contrasting pea (Pisum arvense, P. Sativum and P. Fulvum) species to salt stress: Assessment of variation in antioxidative defence and miRNA expression" in Australian Journal of Crop Science, 7, no. 13 (2013):2145-2153, https://hdl.handle.net/21.15107/rcub_imagine_688 .