Effect of steroids on transcription and secretion of Gal-1 by the human trophoblast cell line in vitro
Abstract
Galectin-1 (Gal-1) is a lectin with recently documented pro-invasive function in trophoblasts in vitro, whose regulation is currently insufficiently known. The potential involvement of steroid hormones, synthetic glucocorticoid dexamethasone (DEX), the sex steroid progesterone (PRG) and mifepristone (RU486) in the regulation of Gal-1 in the trophoblast-derived cell line HTR-8/SVneo was investigated. Gal-1 mRNA levels were assessed by real-time PCR. The effect on secretion of Gal-1 into the culture media was followed using the SELDI-TOF protein chip array. We present evidence that DEX and RU486 significantly reduced Gal-1 in the HTR-8/SVneo cell line at the mRNA level. In addition, trophoblast-derived HTR-8/SVneo cells were shown to secrete detectable Gal-1 protein, which was only slightly increased by PRG. The potential clinical relevance of these findings remains to be determined.
Keywords:
trophoblast / steroids / regulation / HTR-8/SVneo cells / Galectin-1Source:
Archives of Biological Sciences, 2013, 65, 4, 1331-1337Publisher:
- Srpsko biološko društvo, Beograd, i dr.
Funding / projects:
- Trophoblast and extraembryonic fetal cells: plasticity, differentiation factors and in vitro modulation of functional characteristics (RS-MESTD-Basic Research (BR or ON)-173004)
DOI: 10.2298/ABS1304331C
ISSN: 0354-4664
WoS: 000330326600009
Scopus: 2-s2.0-84884694175
Collections
Institution/Community
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Ćujić, Danica AU - Bojić-Trbojević, Žanka AU - Tošić, Nataša AU - Pavlović, Sonja AU - Vićovac, Ljiljana PY - 2013 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/689 AB - Galectin-1 (Gal-1) is a lectin with recently documented pro-invasive function in trophoblasts in vitro, whose regulation is currently insufficiently known. The potential involvement of steroid hormones, synthetic glucocorticoid dexamethasone (DEX), the sex steroid progesterone (PRG) and mifepristone (RU486) in the regulation of Gal-1 in the trophoblast-derived cell line HTR-8/SVneo was investigated. Gal-1 mRNA levels were assessed by real-time PCR. The effect on secretion of Gal-1 into the culture media was followed using the SELDI-TOF protein chip array. We present evidence that DEX and RU486 significantly reduced Gal-1 in the HTR-8/SVneo cell line at the mRNA level. In addition, trophoblast-derived HTR-8/SVneo cells were shown to secrete detectable Gal-1 protein, which was only slightly increased by PRG. The potential clinical relevance of these findings remains to be determined. PB - Srpsko biološko društvo, Beograd, i dr. T2 - Archives of Biological Sciences T1 - Effect of steroids on transcription and secretion of Gal-1 by the human trophoblast cell line in vitro EP - 1337 IS - 4 SP - 1331 VL - 65 DO - 10.2298/ABS1304331C ER -
@article{ author = "Ćujić, Danica and Bojić-Trbojević, Žanka and Tošić, Nataša and Pavlović, Sonja and Vićovac, Ljiljana", year = "2013", abstract = "Galectin-1 (Gal-1) is a lectin with recently documented pro-invasive function in trophoblasts in vitro, whose regulation is currently insufficiently known. The potential involvement of steroid hormones, synthetic glucocorticoid dexamethasone (DEX), the sex steroid progesterone (PRG) and mifepristone (RU486) in the regulation of Gal-1 in the trophoblast-derived cell line HTR-8/SVneo was investigated. Gal-1 mRNA levels were assessed by real-time PCR. The effect on secretion of Gal-1 into the culture media was followed using the SELDI-TOF protein chip array. We present evidence that DEX and RU486 significantly reduced Gal-1 in the HTR-8/SVneo cell line at the mRNA level. In addition, trophoblast-derived HTR-8/SVneo cells were shown to secrete detectable Gal-1 protein, which was only slightly increased by PRG. The potential clinical relevance of these findings remains to be determined.", publisher = "Srpsko biološko društvo, Beograd, i dr.", journal = "Archives of Biological Sciences", title = "Effect of steroids on transcription and secretion of Gal-1 by the human trophoblast cell line in vitro", pages = "1337-1331", number = "4", volume = "65", doi = "10.2298/ABS1304331C" }
Ćujić, D., Bojić-Trbojević, Ž., Tošić, N., Pavlović, S.,& Vićovac, L.. (2013). Effect of steroids on transcription and secretion of Gal-1 by the human trophoblast cell line in vitro. in Archives of Biological Sciences Srpsko biološko društvo, Beograd, i dr.., 65(4), 1331-1337. https://doi.org/10.2298/ABS1304331C
Ćujić D, Bojić-Trbojević Ž, Tošić N, Pavlović S, Vićovac L. Effect of steroids on transcription and secretion of Gal-1 by the human trophoblast cell line in vitro. in Archives of Biological Sciences. 2013;65(4):1331-1337. doi:10.2298/ABS1304331C .
Ćujić, Danica, Bojić-Trbojević, Žanka, Tošić, Nataša, Pavlović, Sonja, Vićovac, Ljiljana, "Effect of steroids on transcription and secretion of Gal-1 by the human trophoblast cell line in vitro" in Archives of Biological Sciences, 65, no. 4 (2013):1331-1337, https://doi.org/10.2298/ABS1304331C . .