Initiation of Meiotic Recombination in Ustilago maydis
Апстракт
A central feature of meiosis is the pairing and recombination of homologous chromosomes. Ustilago maydis, a biotrophic fungus that parasitizes maize, has long been utilized as an experimental system for studying recombination, but it has not been clear when in the life cycle meiotic recombination initiates. U. maydis forms dormant diploid teliospores as the end product of the infection process. Upon germination, teliospores complete meiosis to produce four haploid basidiospores. Here we asked whether the meiotic process begins when teliospores germinate or at an earlier stage in development. When teliospores homozygous for a cdc45 mutation temperature sensitive for DNA synthesis were germinated at the restrictive temperature, four nuclei became visible. This implies that teliospores have already undergone premeiotic DNA synthesis and suggests that meiotic recombination initiates at a stage of infection before teliospores mature. Determination of homologous recombination in plant tissue... infected with U. maydis strains heteroallelic for the nar1 gene revealed that Nar(+) recombinants were produced at a stage before teliospore maturation. Teliospores obtained from a spo11 cross were still able to germinate but the process was highly disturbed and the meiotic products were imbalanced in chromosomal complement. These results show that in U. maydis, homologous recombination initiates during the infection process and that meiosis can proceed even in the absence of Spo11, but with loss of genomic integrity.
Кључне речи:
teliospore / Spo11 / meiosis / homologous recombination / Cdc45Извор:
Genetics, 2013, 195, 4, 1231-1240Издавач:
- Genetics Soc Am, Bethesda
Финансирање / пројекти:
- National Institutes of Health [GM042482, GM079859]
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM042482, R01GM079859] Funding Source: NIH RePORTER
DOI: 10.1534/genetics.113.156752
ISSN: 1943-2631
PubMed: 24077302
WoS: 000328816400004
Scopus: 2-s2.0-84889055104
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Kojić, Milorad AU - Sutherland, Jeanette H. AU - Perez-Martin, Jose AU - Holloman, William K. PY - 2013 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/698 AB - A central feature of meiosis is the pairing and recombination of homologous chromosomes. Ustilago maydis, a biotrophic fungus that parasitizes maize, has long been utilized as an experimental system for studying recombination, but it has not been clear when in the life cycle meiotic recombination initiates. U. maydis forms dormant diploid teliospores as the end product of the infection process. Upon germination, teliospores complete meiosis to produce four haploid basidiospores. Here we asked whether the meiotic process begins when teliospores germinate or at an earlier stage in development. When teliospores homozygous for a cdc45 mutation temperature sensitive for DNA synthesis were germinated at the restrictive temperature, four nuclei became visible. This implies that teliospores have already undergone premeiotic DNA synthesis and suggests that meiotic recombination initiates at a stage of infection before teliospores mature. Determination of homologous recombination in plant tissue infected with U. maydis strains heteroallelic for the nar1 gene revealed that Nar(+) recombinants were produced at a stage before teliospore maturation. Teliospores obtained from a spo11 cross were still able to germinate but the process was highly disturbed and the meiotic products were imbalanced in chromosomal complement. These results show that in U. maydis, homologous recombination initiates during the infection process and that meiosis can proceed even in the absence of Spo11, but with loss of genomic integrity. PB - Genetics Soc Am, Bethesda T2 - Genetics T1 - Initiation of Meiotic Recombination in Ustilago maydis EP - 1240 IS - 4 SP - 1231 VL - 195 DO - 10.1534/genetics.113.156752 ER -
@article{ author = "Kojić, Milorad and Sutherland, Jeanette H. and Perez-Martin, Jose and Holloman, William K.", year = "2013", abstract = "A central feature of meiosis is the pairing and recombination of homologous chromosomes. Ustilago maydis, a biotrophic fungus that parasitizes maize, has long been utilized as an experimental system for studying recombination, but it has not been clear when in the life cycle meiotic recombination initiates. U. maydis forms dormant diploid teliospores as the end product of the infection process. Upon germination, teliospores complete meiosis to produce four haploid basidiospores. Here we asked whether the meiotic process begins when teliospores germinate or at an earlier stage in development. When teliospores homozygous for a cdc45 mutation temperature sensitive for DNA synthesis were germinated at the restrictive temperature, four nuclei became visible. This implies that teliospores have already undergone premeiotic DNA synthesis and suggests that meiotic recombination initiates at a stage of infection before teliospores mature. Determination of homologous recombination in plant tissue infected with U. maydis strains heteroallelic for the nar1 gene revealed that Nar(+) recombinants were produced at a stage before teliospore maturation. Teliospores obtained from a spo11 cross were still able to germinate but the process was highly disturbed and the meiotic products were imbalanced in chromosomal complement. These results show that in U. maydis, homologous recombination initiates during the infection process and that meiosis can proceed even in the absence of Spo11, but with loss of genomic integrity.", publisher = "Genetics Soc Am, Bethesda", journal = "Genetics", title = "Initiation of Meiotic Recombination in Ustilago maydis", pages = "1240-1231", number = "4", volume = "195", doi = "10.1534/genetics.113.156752" }
Kojić, M., Sutherland, J. H., Perez-Martin, J.,& Holloman, W. K.. (2013). Initiation of Meiotic Recombination in Ustilago maydis. in Genetics Genetics Soc Am, Bethesda., 195(4), 1231-1240. https://doi.org/10.1534/genetics.113.156752
Kojić M, Sutherland JH, Perez-Martin J, Holloman WK. Initiation of Meiotic Recombination in Ustilago maydis. in Genetics. 2013;195(4):1231-1240. doi:10.1534/genetics.113.156752 .
Kojić, Milorad, Sutherland, Jeanette H., Perez-Martin, Jose, Holloman, William K., "Initiation of Meiotic Recombination in Ustilago maydis" in Genetics, 195, no. 4 (2013):1231-1240, https://doi.org/10.1534/genetics.113.156752 . .