Investigation of the microbial diversity of an extremely acidic, metal-rich water body (Lake Robule, Bor, Serbia)
2014
Аутори
Stanković, SrdanMorić, Ivana
Pavić, Aleksandar
Vasiljević, Branka
Johnson, D. Barrie
Cvetković, Vladica
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
An investigation of the microbial diversity in the extremely acidic, metal-rich Lake Robule was performed using culture-dependant and culture-independent (T-RFLP) methods. In addition, the ability of the indigenous bacteria from the lake water to leach copper from a mineral concentrate was tested. T-RFLP analysis revealed that the dominant bacteria in the lake water samples were the obligate heterotroph Acidiphilium cryptum (approximate to 50 % of the total bacteria) and the iron-oxidizing autotroph Leptospirillum ferrooxidans (approximate to 40 %) The iron/sulfur-oxidizing autotroph Acidithiobacillus ferrooxidans was reported to be the most abundant bacteria in the Lake in an earlier study, but it was not detected in the present study using T-RFLP, although it was isolated on solid media and detected in enrichment (bioleaching) cultures. The presence of the two bacterial species detected by T-RFLP (L. ferrooxidans and A. cryptum) was also confirmed by cultivation on solid media. The p...resence and relative abundance of the bacteria inhabiting Lake Robule was explained by the physiological characteristics of the bacteria and the physico-chemical characteristics of the lake water.
Кључне речи:
T-RFLP / tailings / biomining / bioleaching / acidophilesИзвор:
Journal of the Serbian Chemical Society, 2014, 79, 6, 729-741Издавач:
- Srpsko hemijsko društvo, Beograd
Финансирање / пројекти:
- Serbian Academy of Sciences and Arts (Project Geodynamics)
- Магматизам и геодинамика Балканског полуострва од мезозоика до данас: значај за образовање металичних и неметаличних рудних лежишта (RS-MESTD-Basic Research (BR or ON)-176016)
- Изучавање микробиолошког диверзитета и карактеризација корисних срединских микроорганизама (RS-MESTD-Basic Research (BR or ON)-173048)
DOI: 10.2298/JSC130605071S
ISSN: 0352-5139
WoS: 000338268800010
Scopus: 2-s2.0-84903155426
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Stanković, Srdan AU - Morić, Ivana AU - Pavić, Aleksandar AU - Vasiljević, Branka AU - Johnson, D. Barrie AU - Cvetković, Vladica PY - 2014 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/712 AB - An investigation of the microbial diversity in the extremely acidic, metal-rich Lake Robule was performed using culture-dependant and culture-independent (T-RFLP) methods. In addition, the ability of the indigenous bacteria from the lake water to leach copper from a mineral concentrate was tested. T-RFLP analysis revealed that the dominant bacteria in the lake water samples were the obligate heterotroph Acidiphilium cryptum (approximate to 50 % of the total bacteria) and the iron-oxidizing autotroph Leptospirillum ferrooxidans (approximate to 40 %) The iron/sulfur-oxidizing autotroph Acidithiobacillus ferrooxidans was reported to be the most abundant bacteria in the Lake in an earlier study, but it was not detected in the present study using T-RFLP, although it was isolated on solid media and detected in enrichment (bioleaching) cultures. The presence of the two bacterial species detected by T-RFLP (L. ferrooxidans and A. cryptum) was also confirmed by cultivation on solid media. The presence and relative abundance of the bacteria inhabiting Lake Robule was explained by the physiological characteristics of the bacteria and the physico-chemical characteristics of the lake water. PB - Srpsko hemijsko društvo, Beograd T2 - Journal of the Serbian Chemical Society T1 - Investigation of the microbial diversity of an extremely acidic, metal-rich water body (Lake Robule, Bor, Serbia) EP - 741 IS - 6 SP - 729 VL - 79 DO - 10.2298/JSC130605071S ER -
@article{ author = "Stanković, Srdan and Morić, Ivana and Pavić, Aleksandar and Vasiljević, Branka and Johnson, D. Barrie and Cvetković, Vladica", year = "2014", abstract = "An investigation of the microbial diversity in the extremely acidic, metal-rich Lake Robule was performed using culture-dependant and culture-independent (T-RFLP) methods. In addition, the ability of the indigenous bacteria from the lake water to leach copper from a mineral concentrate was tested. T-RFLP analysis revealed that the dominant bacteria in the lake water samples were the obligate heterotroph Acidiphilium cryptum (approximate to 50 % of the total bacteria) and the iron-oxidizing autotroph Leptospirillum ferrooxidans (approximate to 40 %) The iron/sulfur-oxidizing autotroph Acidithiobacillus ferrooxidans was reported to be the most abundant bacteria in the Lake in an earlier study, but it was not detected in the present study using T-RFLP, although it was isolated on solid media and detected in enrichment (bioleaching) cultures. The presence of the two bacterial species detected by T-RFLP (L. ferrooxidans and A. cryptum) was also confirmed by cultivation on solid media. The presence and relative abundance of the bacteria inhabiting Lake Robule was explained by the physiological characteristics of the bacteria and the physico-chemical characteristics of the lake water.", publisher = "Srpsko hemijsko društvo, Beograd", journal = "Journal of the Serbian Chemical Society", title = "Investigation of the microbial diversity of an extremely acidic, metal-rich water body (Lake Robule, Bor, Serbia)", pages = "741-729", number = "6", volume = "79", doi = "10.2298/JSC130605071S" }
Stanković, S., Morić, I., Pavić, A., Vasiljević, B., Johnson, D. B.,& Cvetković, V.. (2014). Investigation of the microbial diversity of an extremely acidic, metal-rich water body (Lake Robule, Bor, Serbia). in Journal of the Serbian Chemical Society Srpsko hemijsko društvo, Beograd., 79(6), 729-741. https://doi.org/10.2298/JSC130605071S
Stanković S, Morić I, Pavić A, Vasiljević B, Johnson DB, Cvetković V. Investigation of the microbial diversity of an extremely acidic, metal-rich water body (Lake Robule, Bor, Serbia). in Journal of the Serbian Chemical Society. 2014;79(6):729-741. doi:10.2298/JSC130605071S .
Stanković, Srdan, Morić, Ivana, Pavić, Aleksandar, Vasiljević, Branka, Johnson, D. Barrie, Cvetković, Vladica, "Investigation of the microbial diversity of an extremely acidic, metal-rich water body (Lake Robule, Bor, Serbia)" in Journal of the Serbian Chemical Society, 79, no. 6 (2014):729-741, https://doi.org/10.2298/JSC130605071S . .