Cyclic AMP response element binding (CREB) protein acts as a positive regulator of SOX3 gene expression in NT2/D1 cells
2014
Аутори
Kovačević Grujičić, NatašaMojsin, Marija
Popović, Jelena
Petrović, Isidora
Topalović, Vladanka
Stevanović, Milena
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
SOX3 is one of the earliest neural markers in vertebrates, playing the role in specifying neuronal fate. In this study we have established first functional link between CREB and human SOX3 gene which both have important roles in the nervous system throughout development and in the adulthood. Here we demonstrate both in vitro and in vivo that CREB binds to CRE half-site located -195 to -191 within the human SOX3 promoter. Overexpression studies with CREB or its dominant-negative inhibitor A-CREB indicate that this transcription factor acts as a positive regulator of basal SOX3 gene expression in NT2/D1 cells. This is further confirmed by mutational analysis where mutation of CREB binding site results in reduction of SOX3 promoter activity. Our results point at CREB as a positive regulator of SOX3 gene transcription in NT2/D1 cells, while its contribution to RA induction of SOX3 promoter is not prominent.
Кључне речи:
Transcription regulation / SOX3 / Retinoic acid / NT2/D1 cells / CREBИзвор:
BMB Reports, 2014, 47, 4, 197-202Издавач:
- Korean Society Biochemistry & Molecular Biology, Seoul
Финансирање / пројекти:
- Проучавање сигналних путева и епигенетичких механизама укључених у контролу експресије хуманих SOX гена: даље расветљавање њихове улоге у одређивању судбине и диференцијацији ћелија (RS-MESTD-Basic Research (BR or ON)-173051)
DOI: 10.5483/BMBRep.2014.47.4.084
ISSN: 1976-6696
PubMed: 24257117
WoS: 000335653100004
Scopus: 2-s2.0-84899645172
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Kovačević Grujičić, Nataša AU - Mojsin, Marija AU - Popović, Jelena AU - Petrović, Isidora AU - Topalović, Vladanka AU - Stevanović, Milena PY - 2014 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/723 AB - SOX3 is one of the earliest neural markers in vertebrates, playing the role in specifying neuronal fate. In this study we have established first functional link between CREB and human SOX3 gene which both have important roles in the nervous system throughout development and in the adulthood. Here we demonstrate both in vitro and in vivo that CREB binds to CRE half-site located -195 to -191 within the human SOX3 promoter. Overexpression studies with CREB or its dominant-negative inhibitor A-CREB indicate that this transcription factor acts as a positive regulator of basal SOX3 gene expression in NT2/D1 cells. This is further confirmed by mutational analysis where mutation of CREB binding site results in reduction of SOX3 promoter activity. Our results point at CREB as a positive regulator of SOX3 gene transcription in NT2/D1 cells, while its contribution to RA induction of SOX3 promoter is not prominent. PB - Korean Society Biochemistry & Molecular Biology, Seoul T2 - BMB Reports T1 - Cyclic AMP response element binding (CREB) protein acts as a positive regulator of SOX3 gene expression in NT2/D1 cells EP - 202 IS - 4 SP - 197 VL - 47 DO - 10.5483/BMBRep.2014.47.4.084 ER -
@article{ author = "Kovačević Grujičić, Nataša and Mojsin, Marija and Popović, Jelena and Petrović, Isidora and Topalović, Vladanka and Stevanović, Milena", year = "2014", abstract = "SOX3 is one of the earliest neural markers in vertebrates, playing the role in specifying neuronal fate. In this study we have established first functional link between CREB and human SOX3 gene which both have important roles in the nervous system throughout development and in the adulthood. Here we demonstrate both in vitro and in vivo that CREB binds to CRE half-site located -195 to -191 within the human SOX3 promoter. Overexpression studies with CREB or its dominant-negative inhibitor A-CREB indicate that this transcription factor acts as a positive regulator of basal SOX3 gene expression in NT2/D1 cells. This is further confirmed by mutational analysis where mutation of CREB binding site results in reduction of SOX3 promoter activity. Our results point at CREB as a positive regulator of SOX3 gene transcription in NT2/D1 cells, while its contribution to RA induction of SOX3 promoter is not prominent.", publisher = "Korean Society Biochemistry & Molecular Biology, Seoul", journal = "BMB Reports", title = "Cyclic AMP response element binding (CREB) protein acts as a positive regulator of SOX3 gene expression in NT2/D1 cells", pages = "202-197", number = "4", volume = "47", doi = "10.5483/BMBRep.2014.47.4.084" }
Kovačević Grujičić, N., Mojsin, M., Popović, J., Petrović, I., Topalović, V.,& Stevanović, M.. (2014). Cyclic AMP response element binding (CREB) protein acts as a positive regulator of SOX3 gene expression in NT2/D1 cells. in BMB Reports Korean Society Biochemistry & Molecular Biology, Seoul., 47(4), 197-202. https://doi.org/10.5483/BMBRep.2014.47.4.084
Kovačević Grujičić N, Mojsin M, Popović J, Petrović I, Topalović V, Stevanović M. Cyclic AMP response element binding (CREB) protein acts as a positive regulator of SOX3 gene expression in NT2/D1 cells. in BMB Reports. 2014;47(4):197-202. doi:10.5483/BMBRep.2014.47.4.084 .
Kovačević Grujičić, Nataša, Mojsin, Marija, Popović, Jelena, Petrović, Isidora, Topalović, Vladanka, Stevanović, Milena, "Cyclic AMP response element binding (CREB) protein acts as a positive regulator of SOX3 gene expression in NT2/D1 cells" in BMB Reports, 47, no. 4 (2014):197-202, https://doi.org/10.5483/BMBRep.2014.47.4.084 . .