Cytotoxic effect of Reseda lutea L.: A case of forgotten remedy
Samo za registrovane korisnike
2014
Autori
Radulović, Niko S.Zlatković, Dragan B.
Ilić-Tomić, Tatjana
Šenerović, Lidija
Nikodinović-Runić, Jasmina
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Ethnopharmacological relevance: Reseda lutea L (Resedaceae) or Wild Mignonette is a widely distributed plant species. Pliny the Elder (AD 23-AD 79), a Roman scholar and naturalist, reported the use of R. lutea for reducing tumors in his Historia naturalis. Accounts of the beneficial effects of R. lutea in tumor treatment could also be found in the works of later authors, such as Etienne Francois Geoffroy (1672-1731) and Samuel Frederick Gray (1766-1828). However, to date no in vivo or in vitro evidence exists in support of the alleged tumor healing properties of R. lutea. Materials and methods: The composition of autolysates obtained from different organs (root, flower and fruit) of R. lutea was investigated by GC and GC-MS analyses and IR, 1D and 2D NMR spectroscopy. These analyses led to the discovery of a new compound isolated in pure form from the flower autolysate. Autolysates and their major constituents were submitted to MU-dye reduction cytotoxic assay on human A375 (melanoma) ...and MRC5 (fibroblast) cell lines. Mechanism of the cytotoxic effects was studied by cell cycle analysis and Annexin V assay. Results: Benzyl isothiocyanate and 2-(alpha-L-rhamnopyranosyloxy)benzyl isothiocyanate were identified as the major constituents of the root and flower autolysates, respectively (the later represents a new natural product). These compounds showed significant antiproliferative effects against both cell lines, which could also explain the observed high cytotoxic activity of the tested autolysates. Cell cycle analysis revealed apoptosis as the probable mechanism of cell death. Conclusions: Tumor healing properties attributed to R. lutea in the pre-modern texts were substantiated by the herein obtained results. Two isothiocyanates were found to be the major carriers of the observed activity. Although there was a relatively low differential effect of the plant metabolites on transformed and non-transformed cell lines, one can argue that the noted strong cytotoxicity provides first evidence that could explain the long forgotten use of this particular species.
Ključne reči:
Structure elucidation / Reseda lutea L. / Isothiocyanates / Cytotoxic effect / ApoptosisIzvor:
Journal of Ethnopharmacology, 2014, 153, 1, 125-132Izdavač:
- Elsevier Ireland Ltd, Clare
Finansiranje / projekti:
- Kombinatorne biblioteke heterogenih katalizatora, prirodnih proizvoda, modifikovanih prirodnih proizvoda i njihovih analoga: put ka novim biološki aktivnim agensima (RS-MESTD-Basic Research (BR or ON)-172061)
- Izučavanje mikrobiološkog diverziteta i karakterizacija korisnih sredinskih mikroorganizama (RS-MESTD-Basic Research (BR or ON)-173048)
DOI: 10.1016/j.jep.2014.01.034
ISSN: 0378-8741
PubMed: 24509155
WoS: 000335428500010
Scopus: 2-s2.0-84897110717
Institucija/grupa
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Radulović, Niko S. AU - Zlatković, Dragan B. AU - Ilić-Tomić, Tatjana AU - Šenerović, Lidija AU - Nikodinović-Runić, Jasmina PY - 2014 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/724 AB - Ethnopharmacological relevance: Reseda lutea L (Resedaceae) or Wild Mignonette is a widely distributed plant species. Pliny the Elder (AD 23-AD 79), a Roman scholar and naturalist, reported the use of R. lutea for reducing tumors in his Historia naturalis. Accounts of the beneficial effects of R. lutea in tumor treatment could also be found in the works of later authors, such as Etienne Francois Geoffroy (1672-1731) and Samuel Frederick Gray (1766-1828). However, to date no in vivo or in vitro evidence exists in support of the alleged tumor healing properties of R. lutea. Materials and methods: The composition of autolysates obtained from different organs (root, flower and fruit) of R. lutea was investigated by GC and GC-MS analyses and IR, 1D and 2D NMR spectroscopy. These analyses led to the discovery of a new compound isolated in pure form from the flower autolysate. Autolysates and their major constituents were submitted to MU-dye reduction cytotoxic assay on human A375 (melanoma) and MRC5 (fibroblast) cell lines. Mechanism of the cytotoxic effects was studied by cell cycle analysis and Annexin V assay. Results: Benzyl isothiocyanate and 2-(alpha-L-rhamnopyranosyloxy)benzyl isothiocyanate were identified as the major constituents of the root and flower autolysates, respectively (the later represents a new natural product). These compounds showed significant antiproliferative effects against both cell lines, which could also explain the observed high cytotoxic activity of the tested autolysates. Cell cycle analysis revealed apoptosis as the probable mechanism of cell death. Conclusions: Tumor healing properties attributed to R. lutea in the pre-modern texts were substantiated by the herein obtained results. Two isothiocyanates were found to be the major carriers of the observed activity. Although there was a relatively low differential effect of the plant metabolites on transformed and non-transformed cell lines, one can argue that the noted strong cytotoxicity provides first evidence that could explain the long forgotten use of this particular species. PB - Elsevier Ireland Ltd, Clare T2 - Journal of Ethnopharmacology T1 - Cytotoxic effect of Reseda lutea L.: A case of forgotten remedy EP - 132 IS - 1 SP - 125 VL - 153 DO - 10.1016/j.jep.2014.01.034 ER -
@article{ author = "Radulović, Niko S. and Zlatković, Dragan B. and Ilić-Tomić, Tatjana and Šenerović, Lidija and Nikodinović-Runić, Jasmina", year = "2014", abstract = "Ethnopharmacological relevance: Reseda lutea L (Resedaceae) or Wild Mignonette is a widely distributed plant species. Pliny the Elder (AD 23-AD 79), a Roman scholar and naturalist, reported the use of R. lutea for reducing tumors in his Historia naturalis. Accounts of the beneficial effects of R. lutea in tumor treatment could also be found in the works of later authors, such as Etienne Francois Geoffroy (1672-1731) and Samuel Frederick Gray (1766-1828). However, to date no in vivo or in vitro evidence exists in support of the alleged tumor healing properties of R. lutea. Materials and methods: The composition of autolysates obtained from different organs (root, flower and fruit) of R. lutea was investigated by GC and GC-MS analyses and IR, 1D and 2D NMR spectroscopy. These analyses led to the discovery of a new compound isolated in pure form from the flower autolysate. Autolysates and their major constituents were submitted to MU-dye reduction cytotoxic assay on human A375 (melanoma) and MRC5 (fibroblast) cell lines. Mechanism of the cytotoxic effects was studied by cell cycle analysis and Annexin V assay. Results: Benzyl isothiocyanate and 2-(alpha-L-rhamnopyranosyloxy)benzyl isothiocyanate were identified as the major constituents of the root and flower autolysates, respectively (the later represents a new natural product). These compounds showed significant antiproliferative effects against both cell lines, which could also explain the observed high cytotoxic activity of the tested autolysates. Cell cycle analysis revealed apoptosis as the probable mechanism of cell death. Conclusions: Tumor healing properties attributed to R. lutea in the pre-modern texts were substantiated by the herein obtained results. Two isothiocyanates were found to be the major carriers of the observed activity. Although there was a relatively low differential effect of the plant metabolites on transformed and non-transformed cell lines, one can argue that the noted strong cytotoxicity provides first evidence that could explain the long forgotten use of this particular species.", publisher = "Elsevier Ireland Ltd, Clare", journal = "Journal of Ethnopharmacology", title = "Cytotoxic effect of Reseda lutea L.: A case of forgotten remedy", pages = "132-125", number = "1", volume = "153", doi = "10.1016/j.jep.2014.01.034" }
Radulović, N. S., Zlatković, D. B., Ilić-Tomić, T., Šenerović, L.,& Nikodinović-Runić, J.. (2014). Cytotoxic effect of Reseda lutea L.: A case of forgotten remedy. in Journal of Ethnopharmacology Elsevier Ireland Ltd, Clare., 153(1), 125-132. https://doi.org/10.1016/j.jep.2014.01.034
Radulović NS, Zlatković DB, Ilić-Tomić T, Šenerović L, Nikodinović-Runić J. Cytotoxic effect of Reseda lutea L.: A case of forgotten remedy. in Journal of Ethnopharmacology. 2014;153(1):125-132. doi:10.1016/j.jep.2014.01.034 .
Radulović, Niko S., Zlatković, Dragan B., Ilić-Tomić, Tatjana, Šenerović, Lidija, Nikodinović-Runić, Jasmina, "Cytotoxic effect of Reseda lutea L.: A case of forgotten remedy" in Journal of Ethnopharmacology, 153, no. 1 (2014):125-132, https://doi.org/10.1016/j.jep.2014.01.034 . .