The Telomerase Reverse Transcriptase Subunit from the Dimorphic Fungus Ustilago maydis
2014
Аутори
Bautista-Espana, DoloresAnastacio-Marcelino, Estela
Horta-Valerdi, Guillermo
Celestino-Montes, Antonio
Kojić, Milorad
Negrete-Abascal, Erasmo
Reyes-Cervantes, Hortensia
Vazquez-Cruz, Candelario
Guzman, Plinio
Sanchez-Alonso, Patricia
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
In this study, we investigated the reverse transcriptase subunit of telomerase in the dimorphic fungus Ustilago maydis. This protein (Trt1) contains 1371 amino acids and all of the characteristic TERT motifs. Mutants created by disrupting trt1 had senescent traits, such as delayed growth, low replicative potential, and reduced survival, that were reminiscent of the traits observed in est2 budding yeast mutants. Telomerase activity was observed in wild-type fungus sporidia but not those of the disruption mutant. The introduction of a self-replicating plasmid expressing Trt1 into the mutant strain restored growth proficiency and replicative potential. Analyses of trt1 crosses in planta suggested that Trt1 is necessary for teliospore formation in homozygous disrupted diploids and that telomerase is haploinsufficient in heterozygous diploids. Additionally, terminal restriction fragment analysis in the progeny hinted at alternative survival mechanisms similar to those of budding yeast.
Извор:
PLoS One, 2014, 9, 10Издавач:
- Public Library Science, San Francisco
Финансирање / пројекти:
- CONACyT [46378-Q, CB106140-P2]
DOI: 10.1371/journal.pone.0109981
ISSN: 1932-6203
PubMed: 25299159
WoS: 000343941200091
Scopus: 2-s2.0-84907842497
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Bautista-Espana, Dolores AU - Anastacio-Marcelino, Estela AU - Horta-Valerdi, Guillermo AU - Celestino-Montes, Antonio AU - Kojić, Milorad AU - Negrete-Abascal, Erasmo AU - Reyes-Cervantes, Hortensia AU - Vazquez-Cruz, Candelario AU - Guzman, Plinio AU - Sanchez-Alonso, Patricia PY - 2014 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/726 AB - In this study, we investigated the reverse transcriptase subunit of telomerase in the dimorphic fungus Ustilago maydis. This protein (Trt1) contains 1371 amino acids and all of the characteristic TERT motifs. Mutants created by disrupting trt1 had senescent traits, such as delayed growth, low replicative potential, and reduced survival, that were reminiscent of the traits observed in est2 budding yeast mutants. Telomerase activity was observed in wild-type fungus sporidia but not those of the disruption mutant. The introduction of a self-replicating plasmid expressing Trt1 into the mutant strain restored growth proficiency and replicative potential. Analyses of trt1 crosses in planta suggested that Trt1 is necessary for teliospore formation in homozygous disrupted diploids and that telomerase is haploinsufficient in heterozygous diploids. Additionally, terminal restriction fragment analysis in the progeny hinted at alternative survival mechanisms similar to those of budding yeast. PB - Public Library Science, San Francisco T2 - PLoS One T1 - The Telomerase Reverse Transcriptase Subunit from the Dimorphic Fungus Ustilago maydis IS - 10 VL - 9 DO - 10.1371/journal.pone.0109981 ER -
@article{ author = "Bautista-Espana, Dolores and Anastacio-Marcelino, Estela and Horta-Valerdi, Guillermo and Celestino-Montes, Antonio and Kojić, Milorad and Negrete-Abascal, Erasmo and Reyes-Cervantes, Hortensia and Vazquez-Cruz, Candelario and Guzman, Plinio and Sanchez-Alonso, Patricia", year = "2014", abstract = "In this study, we investigated the reverse transcriptase subunit of telomerase in the dimorphic fungus Ustilago maydis. This protein (Trt1) contains 1371 amino acids and all of the characteristic TERT motifs. Mutants created by disrupting trt1 had senescent traits, such as delayed growth, low replicative potential, and reduced survival, that were reminiscent of the traits observed in est2 budding yeast mutants. Telomerase activity was observed in wild-type fungus sporidia but not those of the disruption mutant. The introduction of a self-replicating plasmid expressing Trt1 into the mutant strain restored growth proficiency and replicative potential. Analyses of trt1 crosses in planta suggested that Trt1 is necessary for teliospore formation in homozygous disrupted diploids and that telomerase is haploinsufficient in heterozygous diploids. Additionally, terminal restriction fragment analysis in the progeny hinted at alternative survival mechanisms similar to those of budding yeast.", publisher = "Public Library Science, San Francisco", journal = "PLoS One", title = "The Telomerase Reverse Transcriptase Subunit from the Dimorphic Fungus Ustilago maydis", number = "10", volume = "9", doi = "10.1371/journal.pone.0109981" }
Bautista-Espana, D., Anastacio-Marcelino, E., Horta-Valerdi, G., Celestino-Montes, A., Kojić, M., Negrete-Abascal, E., Reyes-Cervantes, H., Vazquez-Cruz, C., Guzman, P.,& Sanchez-Alonso, P.. (2014). The Telomerase Reverse Transcriptase Subunit from the Dimorphic Fungus Ustilago maydis. in PLoS One Public Library Science, San Francisco., 9(10). https://doi.org/10.1371/journal.pone.0109981
Bautista-Espana D, Anastacio-Marcelino E, Horta-Valerdi G, Celestino-Montes A, Kojić M, Negrete-Abascal E, Reyes-Cervantes H, Vazquez-Cruz C, Guzman P, Sanchez-Alonso P. The Telomerase Reverse Transcriptase Subunit from the Dimorphic Fungus Ustilago maydis. in PLoS One. 2014;9(10). doi:10.1371/journal.pone.0109981 .
Bautista-Espana, Dolores, Anastacio-Marcelino, Estela, Horta-Valerdi, Guillermo, Celestino-Montes, Antonio, Kojić, Milorad, Negrete-Abascal, Erasmo, Reyes-Cervantes, Hortensia, Vazquez-Cruz, Candelario, Guzman, Plinio, Sanchez-Alonso, Patricia, "The Telomerase Reverse Transcriptase Subunit from the Dimorphic Fungus Ustilago maydis" in PLoS One, 9, no. 10 (2014), https://doi.org/10.1371/journal.pone.0109981 . .