DNA sequence analysis of spontaneous histidine mutations in a polA1 strain of Escherichia coli K12 suggests a specific role of the GTGG sequence
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1990
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Spontaneously arising histidine mutations in an Escherichia coli K12 strain deficient for DNA polymerase I were analysed at the DNA sequence level. We screened approximately 150000 colonies and isolated 106 histidine auxotrophs. Of these, 98 were unstable hisC mutations; 12 representative mutants analysed were shown to have arisen by the excision of a single quadruplet repeat in the sequence 5′-GCTGGCTGGCTGGCTG-3′. Of the eight mutations at other sites, three hisA deletions and one hisD deletion occurred as a consequence of misalignment of tandemly repeated pentamers (hisD) or decamers (hisA). A single hisA point mutation was found to be a missense mutation. Two extended deletions, covering the his operon were not analysed. We could not identify the hisC deletion by sequencing. We conclude that polA1 is a strong imitator that induces mutations mostly of the minus frameshift and deletion type by a Streisinger-type of mispairing in repetitive DNA sequences. Finally, the possible role of ...a 5′-GTGG-3′ sequence and its inverted or direct complements, which are found in the vicinity of all the deletions and frameshifts, is discussed.
Ključne reči:
Mutagenic specificity / DNA repair / DNA polymerase IIzvor:
MGG Molecular & General Genetics, 1990, 223, 3, 481-486Izdavač:
- Springer-Verlag
DOI: 10.1007/BF00264457
ISSN: 0026-8925
PubMed: 2270088
Scopus: 2-s2.0-0025038454
Institucija/grupa
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Janković, M. AU - Kostić, Tatjana AU - Savić, D.J. PY - 1990 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/72 AB - Spontaneously arising histidine mutations in an Escherichia coli K12 strain deficient for DNA polymerase I were analysed at the DNA sequence level. We screened approximately 150000 colonies and isolated 106 histidine auxotrophs. Of these, 98 were unstable hisC mutations; 12 representative mutants analysed were shown to have arisen by the excision of a single quadruplet repeat in the sequence 5′-GCTGGCTGGCTGGCTG-3′. Of the eight mutations at other sites, three hisA deletions and one hisD deletion occurred as a consequence of misalignment of tandemly repeated pentamers (hisD) or decamers (hisA). A single hisA point mutation was found to be a missense mutation. Two extended deletions, covering the his operon were not analysed. We could not identify the hisC deletion by sequencing. We conclude that polA1 is a strong imitator that induces mutations mostly of the minus frameshift and deletion type by a Streisinger-type of mispairing in repetitive DNA sequences. Finally, the possible role of a 5′-GTGG-3′ sequence and its inverted or direct complements, which are found in the vicinity of all the deletions and frameshifts, is discussed. PB - Springer-Verlag T2 - MGG Molecular & General Genetics T1 - DNA sequence analysis of spontaneous histidine mutations in a polA1 strain of Escherichia coli K12 suggests a specific role of the GTGG sequence EP - 486 IS - 3 SP - 481 VL - 223 DO - 10.1007/BF00264457 ER -
@article{ author = "Janković, M. and Kostić, Tatjana and Savić, D.J.", year = "1990", abstract = "Spontaneously arising histidine mutations in an Escherichia coli K12 strain deficient for DNA polymerase I were analysed at the DNA sequence level. We screened approximately 150000 colonies and isolated 106 histidine auxotrophs. Of these, 98 were unstable hisC mutations; 12 representative mutants analysed were shown to have arisen by the excision of a single quadruplet repeat in the sequence 5′-GCTGGCTGGCTGGCTG-3′. Of the eight mutations at other sites, three hisA deletions and one hisD deletion occurred as a consequence of misalignment of tandemly repeated pentamers (hisD) or decamers (hisA). A single hisA point mutation was found to be a missense mutation. Two extended deletions, covering the his operon were not analysed. We could not identify the hisC deletion by sequencing. We conclude that polA1 is a strong imitator that induces mutations mostly of the minus frameshift and deletion type by a Streisinger-type of mispairing in repetitive DNA sequences. Finally, the possible role of a 5′-GTGG-3′ sequence and its inverted or direct complements, which are found in the vicinity of all the deletions and frameshifts, is discussed.", publisher = "Springer-Verlag", journal = "MGG Molecular & General Genetics", title = "DNA sequence analysis of spontaneous histidine mutations in a polA1 strain of Escherichia coli K12 suggests a specific role of the GTGG sequence", pages = "486-481", number = "3", volume = "223", doi = "10.1007/BF00264457" }
Janković, M., Kostić, T.,& Savić, D.J.. (1990). DNA sequence analysis of spontaneous histidine mutations in a polA1 strain of Escherichia coli K12 suggests a specific role of the GTGG sequence. in MGG Molecular & General Genetics Springer-Verlag., 223(3), 481-486. https://doi.org/10.1007/BF00264457
Janković M, Kostić T, Savić D. DNA sequence analysis of spontaneous histidine mutations in a polA1 strain of Escherichia coli K12 suggests a specific role of the GTGG sequence. in MGG Molecular & General Genetics. 1990;223(3):481-486. doi:10.1007/BF00264457 .
Janković, M., Kostić, Tatjana , Savić, D.J., "DNA sequence analysis of spontaneous histidine mutations in a polA1 strain of Escherichia coli K12 suggests a specific role of the GTGG sequence" in MGG Molecular & General Genetics, 223, no. 3 (1990):481-486, https://doi.org/10.1007/BF00264457 . .