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DNA sequence analysis of spontaneous histidine mutations in a polA1 strain of Escherichia coli K12 suggests a specific role of the GTGG sequence
dc.creator | Janković, M. | |
dc.creator | Kostić, Tatjana | |
dc.creator | Savić, D.J. | |
dc.date.accessioned | 2022-11-15T13:27:26Z | |
dc.date.available | 2022-11-15T13:27:26Z | |
dc.date.issued | 1990 | |
dc.identifier.issn | 0026-8925 | |
dc.identifier.uri | https://imagine.imgge.bg.ac.rs/handle/123456789/72 | |
dc.description.abstract | Spontaneously arising histidine mutations in an Escherichia coli K12 strain deficient for DNA polymerase I were analysed at the DNA sequence level. We screened approximately 150000 colonies and isolated 106 histidine auxotrophs. Of these, 98 were unstable hisC mutations; 12 representative mutants analysed were shown to have arisen by the excision of a single quadruplet repeat in the sequence 5′-GCTGGCTGGCTGGCTG-3′. Of the eight mutations at other sites, three hisA deletions and one hisD deletion occurred as a consequence of misalignment of tandemly repeated pentamers (hisD) or decamers (hisA). A single hisA point mutation was found to be a missense mutation. Two extended deletions, covering the his operon were not analysed. We could not identify the hisC deletion by sequencing. We conclude that polA1 is a strong imitator that induces mutations mostly of the minus frameshift and deletion type by a Streisinger-type of mispairing in repetitive DNA sequences. Finally, the possible role of a 5′-GTGG-3′ sequence and its inverted or direct complements, which are found in the vicinity of all the deletions and frameshifts, is discussed. | en |
dc.publisher | Springer-Verlag | |
dc.rights | restrictedAccess | |
dc.source | MGG Molecular & General Genetics | |
dc.subject | Mutagenic specificity | en |
dc.subject | DNA repair | en |
dc.subject | DNA polymerase I | en |
dc.title | DNA sequence analysis of spontaneous histidine mutations in a polA1 strain of Escherichia coli K12 suggests a specific role of the GTGG sequence | en |
dc.type | article | |
dc.rights.license | ARR | |
dc.citation.epage | 486 | |
dc.citation.issue | 3 | |
dc.citation.other | 223(3): 481-486 | |
dc.citation.spage | 481 | |
dc.citation.volume | 223 | |
dc.identifier.doi | 10.1007/BF00264457 | |
dc.identifier.pmid | 2270088 | |
dc.identifier.scopus | 2-s2.0-0025038454 | |
dc.type.version | publishedVersion |