Molecular tools for utilization of mitochondrial diversity in faba bean (vicia faba)
2014
Authors
Aleksić, Jelena M.Miljuš-Đukić, Jovanka
Jovanović, Živko
Tomić, Branko
Banović Đeri, Bojana
Article (Published version)
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Show full item recordAbstract
We performed in silico PCR analyses utilizing complete mitochondrial (mtDNA) genome sequences of faba bean (Vicia faba) and two related species, Vigna angularis and Vigna radiata, currently available in GenBank, to infer whether 15 published universal primer pairs for amplification of all 14 cis-spliced introns in genes of NADH subunits (nad genes) are suitable for V. faba and related species. Then, we tested via PCR reactions whether seven out of 15 primer pairs would generate PCR products suitable for further manipulation in 16 genotypes of V. faba representing all botanical varieties of this species (major, minor, equina and subsp. paucijuga) of various levels of improvement (traditional and improved cultivars) originating from Europe, Africa, Asia and south America. We provide new PCR primers for amplification of nad1 intron 2/3 in V. faba, and demonstrate intraspecific variability in primary nucleotide sequences at this locus. Based on outcomes of both in silico predictions and PC...R amplification, we report a set of PCR primers for amplification of five introns in nad genes that are promising molecular tools for future phylogeographic and other studies in this species for which unambiguous data on wild ancestors, centre of origin and domestication are lacking.
Keywords:
universal primers / NADH dehydrogenase / mitochondrial genome / introns / in silico PCR / faba beanSource:
Genetika-Belgrade, 2014, 46, 3, 745-762Publisher:
- Društvo genetičara Srbije, Beograd
Funding / projects:
- The Role of Transcription Factors and Small RNAs in Abiotic Stress Response in Plants and Genetic Diversity of Plant Species Important for Agriculture and Biotechnology (RS-MESTD-Basic Research (BR or ON)-173005)
- "Pavle Savic" program [680-00-132/2012-09/15]
DOI: 10.2298/GENSR1403745A
ISSN: 0534-0012
WoS: 000348608200009
Scopus: 2-s2.0-84922109157
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Institution/Community
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Aleksić, Jelena M. AU - Miljuš-Đukić, Jovanka AU - Jovanović, Živko AU - Tomić, Branko AU - Banović Đeri, Bojana PY - 2014 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/732 AB - We performed in silico PCR analyses utilizing complete mitochondrial (mtDNA) genome sequences of faba bean (Vicia faba) and two related species, Vigna angularis and Vigna radiata, currently available in GenBank, to infer whether 15 published universal primer pairs for amplification of all 14 cis-spliced introns in genes of NADH subunits (nad genes) are suitable for V. faba and related species. Then, we tested via PCR reactions whether seven out of 15 primer pairs would generate PCR products suitable for further manipulation in 16 genotypes of V. faba representing all botanical varieties of this species (major, minor, equina and subsp. paucijuga) of various levels of improvement (traditional and improved cultivars) originating from Europe, Africa, Asia and south America. We provide new PCR primers for amplification of nad1 intron 2/3 in V. faba, and demonstrate intraspecific variability in primary nucleotide sequences at this locus. Based on outcomes of both in silico predictions and PCR amplification, we report a set of PCR primers for amplification of five introns in nad genes that are promising molecular tools for future phylogeographic and other studies in this species for which unambiguous data on wild ancestors, centre of origin and domestication are lacking. PB - Društvo genetičara Srbije, Beograd T2 - Genetika-Belgrade T1 - Molecular tools for utilization of mitochondrial diversity in faba bean (vicia faba) EP - 762 IS - 3 SP - 745 VL - 46 DO - 10.2298/GENSR1403745A ER -
@article{ author = "Aleksić, Jelena M. and Miljuš-Đukić, Jovanka and Jovanović, Živko and Tomić, Branko and Banović Đeri, Bojana", year = "2014", abstract = "We performed in silico PCR analyses utilizing complete mitochondrial (mtDNA) genome sequences of faba bean (Vicia faba) and two related species, Vigna angularis and Vigna radiata, currently available in GenBank, to infer whether 15 published universal primer pairs for amplification of all 14 cis-spliced introns in genes of NADH subunits (nad genes) are suitable for V. faba and related species. Then, we tested via PCR reactions whether seven out of 15 primer pairs would generate PCR products suitable for further manipulation in 16 genotypes of V. faba representing all botanical varieties of this species (major, minor, equina and subsp. paucijuga) of various levels of improvement (traditional and improved cultivars) originating from Europe, Africa, Asia and south America. We provide new PCR primers for amplification of nad1 intron 2/3 in V. faba, and demonstrate intraspecific variability in primary nucleotide sequences at this locus. Based on outcomes of both in silico predictions and PCR amplification, we report a set of PCR primers for amplification of five introns in nad genes that are promising molecular tools for future phylogeographic and other studies in this species for which unambiguous data on wild ancestors, centre of origin and domestication are lacking.", publisher = "Društvo genetičara Srbije, Beograd", journal = "Genetika-Belgrade", title = "Molecular tools for utilization of mitochondrial diversity in faba bean (vicia faba)", pages = "762-745", number = "3", volume = "46", doi = "10.2298/GENSR1403745A" }
Aleksić, J. M., Miljuš-Đukić, J., Jovanović, Ž., Tomić, B.,& Banović Đeri, B.. (2014). Molecular tools for utilization of mitochondrial diversity in faba bean (vicia faba). in Genetika-Belgrade Društvo genetičara Srbije, Beograd., 46(3), 745-762. https://doi.org/10.2298/GENSR1403745A
Aleksić JM, Miljuš-Đukić J, Jovanović Ž, Tomić B, Banović Đeri B. Molecular tools for utilization of mitochondrial diversity in faba bean (vicia faba). in Genetika-Belgrade. 2014;46(3):745-762. doi:10.2298/GENSR1403745A .
Aleksić, Jelena M., Miljuš-Đukić, Jovanka, Jovanović, Živko, Tomić, Branko, Banović Đeri, Bojana, "Molecular tools for utilization of mitochondrial diversity in faba bean (vicia faba)" in Genetika-Belgrade, 46, no. 3 (2014):745-762, https://doi.org/10.2298/GENSR1403745A . .