Regulation of ATP-Gated P2X Channels: From Redox Signaling to Interactions with Other Proteins
Abstract
Significance: The family of purinergic P2X receptors (P2XRs) is a part of ligand-gated superfamily of channels activated by extracellular adenosine-5'-triphosphate. P2XRs are present in virtually all mammalian tissues as well as in tissues of other vertebrate and nonvertebrate species and mediate a large variety of functions, including fast transmission at central synapses, contraction of smooth muscle cells, platelet aggregation, and macrophage activation to proliferation and cell death. Recent Advances: The recent solving of crystal structure of the zebrafish P2X4.1R is a major advance in the understanding of structural correlates of channel activation and regulation. Combined with growing information obtained in the post-structure era and the reinterpretation of previous work within the context of the tridimensional structure, these data provide a better understanding of how the channel operates at the molecular levels. Critical Issues: This review focuses on the relationship betwee...n redox signaling and P2XR function. We also discuss other allosteric modulation of P2XR gating in the physiological/pathophysiological context. This includes the summary of extracellular actions of trace metals, which can be released to the synaptic cleft, pH decrease that happens during ischemia and inflammation, and calcium, an extracellular and intracellular messenger. Future Directions: Our evolving understanding of activation and regulation of P2XRs is helpful in clarifying the mechanism by which these channels trigger and modulate cellular functions. Further research is required to identify the signaling pathways contributing to the regulation of the receptor activity and to develop novel and receptor-specific allosteric modulators, which could be used in vivo with therapeutic potential.
Source:
Antioxidants & Redox Signaling, 2014, 21, 6, 953-970Publisher:
- Mary Ann Liebert, Inc, New Rochelle
Funding / projects:
- Intramural Research Program of the National Institute of Child Health and Human Development, NIH
- FONDECYT Initiation [11121302]
- EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT [ZIAHD000195] Funding Source: NIH RePORTER
DOI: 10.1089/ars.2013.5549
ISSN: 1523-0864
PubMed: 23944253
WoS: 000340541700010
Scopus: 2-s2.0-84905042743
Collections
Institution/Community
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Stojilković, Stanko S. AU - Leiva-Salcedo, Elias AU - Rokić, Miloš AU - Coddou, Claudio PY - 2014 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/741 AB - Significance: The family of purinergic P2X receptors (P2XRs) is a part of ligand-gated superfamily of channels activated by extracellular adenosine-5'-triphosphate. P2XRs are present in virtually all mammalian tissues as well as in tissues of other vertebrate and nonvertebrate species and mediate a large variety of functions, including fast transmission at central synapses, contraction of smooth muscle cells, platelet aggregation, and macrophage activation to proliferation and cell death. Recent Advances: The recent solving of crystal structure of the zebrafish P2X4.1R is a major advance in the understanding of structural correlates of channel activation and regulation. Combined with growing information obtained in the post-structure era and the reinterpretation of previous work within the context of the tridimensional structure, these data provide a better understanding of how the channel operates at the molecular levels. Critical Issues: This review focuses on the relationship between redox signaling and P2XR function. We also discuss other allosteric modulation of P2XR gating in the physiological/pathophysiological context. This includes the summary of extracellular actions of trace metals, which can be released to the synaptic cleft, pH decrease that happens during ischemia and inflammation, and calcium, an extracellular and intracellular messenger. Future Directions: Our evolving understanding of activation and regulation of P2XRs is helpful in clarifying the mechanism by which these channels trigger and modulate cellular functions. Further research is required to identify the signaling pathways contributing to the regulation of the receptor activity and to develop novel and receptor-specific allosteric modulators, which could be used in vivo with therapeutic potential. PB - Mary Ann Liebert, Inc, New Rochelle T2 - Antioxidants & Redox Signaling T1 - Regulation of ATP-Gated P2X Channels: From Redox Signaling to Interactions with Other Proteins EP - 970 IS - 6 SP - 953 VL - 21 DO - 10.1089/ars.2013.5549 ER -
@article{ author = "Stojilković, Stanko S. and Leiva-Salcedo, Elias and Rokić, Miloš and Coddou, Claudio", year = "2014", abstract = "Significance: The family of purinergic P2X receptors (P2XRs) is a part of ligand-gated superfamily of channels activated by extracellular adenosine-5'-triphosphate. P2XRs are present in virtually all mammalian tissues as well as in tissues of other vertebrate and nonvertebrate species and mediate a large variety of functions, including fast transmission at central synapses, contraction of smooth muscle cells, platelet aggregation, and macrophage activation to proliferation and cell death. Recent Advances: The recent solving of crystal structure of the zebrafish P2X4.1R is a major advance in the understanding of structural correlates of channel activation and regulation. Combined with growing information obtained in the post-structure era and the reinterpretation of previous work within the context of the tridimensional structure, these data provide a better understanding of how the channel operates at the molecular levels. Critical Issues: This review focuses on the relationship between redox signaling and P2XR function. We also discuss other allosteric modulation of P2XR gating in the physiological/pathophysiological context. This includes the summary of extracellular actions of trace metals, which can be released to the synaptic cleft, pH decrease that happens during ischemia and inflammation, and calcium, an extracellular and intracellular messenger. Future Directions: Our evolving understanding of activation and regulation of P2XRs is helpful in clarifying the mechanism by which these channels trigger and modulate cellular functions. Further research is required to identify the signaling pathways contributing to the regulation of the receptor activity and to develop novel and receptor-specific allosteric modulators, which could be used in vivo with therapeutic potential.", publisher = "Mary Ann Liebert, Inc, New Rochelle", journal = "Antioxidants & Redox Signaling", title = "Regulation of ATP-Gated P2X Channels: From Redox Signaling to Interactions with Other Proteins", pages = "970-953", number = "6", volume = "21", doi = "10.1089/ars.2013.5549" }
Stojilković, S. S., Leiva-Salcedo, E., Rokić, M.,& Coddou, C.. (2014). Regulation of ATP-Gated P2X Channels: From Redox Signaling to Interactions with Other Proteins. in Antioxidants & Redox Signaling Mary Ann Liebert, Inc, New Rochelle., 21(6), 953-970. https://doi.org/10.1089/ars.2013.5549
Stojilković SS, Leiva-Salcedo E, Rokić M, Coddou C. Regulation of ATP-Gated P2X Channels: From Redox Signaling to Interactions with Other Proteins. in Antioxidants & Redox Signaling. 2014;21(6):953-970. doi:10.1089/ars.2013.5549 .
Stojilković, Stanko S., Leiva-Salcedo, Elias, Rokić, Miloš, Coddou, Claudio, "Regulation of ATP-Gated P2X Channels: From Redox Signaling to Interactions with Other Proteins" in Antioxidants & Redox Signaling, 21, no. 6 (2014):953-970, https://doi.org/10.1089/ars.2013.5549 . .