Water deficit down-regulates miR398 and miR408 in pea (Pisum sativum L.)
Authorized Users Only
2014
Authors
Jovanović, ŽivkoStanisavljević, Nemanja
Mikić, Aleksandar
Radović, Svetlana
Maksimović, Vesna R.
Article (Published version)
Metadata
Show full item recordAbstract
MicroRNAs (miRNAs), recently recognized as important regulator of gene expression at post-transcriptional level, have been found to be involved in plant stress responses. The observation that some miRNAs are up- or down regulated by stress implies that they could play vital roles in plant resistance to abiotic and biotic stress. We investigated the effect of water stress treatment during 10 days on expression of conserved miRNAs-miR398a/b and miR408 in pea plants. This time frame reflects the changes as close as possible to the changes where water stress causes visible effects under field condition. It was observed that dehydration strongly down regulates the expression of both miR398a/b and miR408 in pea roots and shoots. The down-regulation of miR398a/b and the up-regulation of potential target - genes copper superoxide dismutase, CSD1, highlight the involvement of this miRNA in pea stress response. To the contrary, the mRNA level of cytochrome c oxidase subunit 5 (COX5b) did not cha...nge in roots and shoots of water-stressed plants, compared to control (well) hydrated plants. This suggests that COX5b is not the target of miR398, or that its expression is regulated by some other mechanism. P-1B-ATPase expression increased during water deficit only in the shoots of pea; in the roots there were no changes in expression. Our results help to understand the possible role of investigated miRNAs and their contribution to pea capacity to cope with water deficit.
Keywords:
Water deficit / Pisum sativum / miR408 / miR398 / microRNAsSource:
Plant Physiology and Biochemistry, 2014, 83, 26-31Publisher:
- Elsevier France-Editions Scientifiques Medicales Elsevier, Paris
Funding / projects:
- The Role of Transcription Factors and Small RNAs in Abiotic Stress Response in Plants and Genetic Diversity of Plant Species Important for Agriculture and Biotechnology (RS-MESTD-Basic Research (BR or ON)-173005)
- SEE ERA NET Project [168-SEELEGUMES]
DOI: 10.1016/j.plaphy.2014.07.008
ISSN: 0981-9428
PubMed: 25064597
WoS: 000343349100004
Scopus: 2-s2.0-84904870627
Collections
Institution/Community
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Jovanović, Živko AU - Stanisavljević, Nemanja AU - Mikić, Aleksandar AU - Radović, Svetlana AU - Maksimović, Vesna R. PY - 2014 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/742 AB - MicroRNAs (miRNAs), recently recognized as important regulator of gene expression at post-transcriptional level, have been found to be involved in plant stress responses. The observation that some miRNAs are up- or down regulated by stress implies that they could play vital roles in plant resistance to abiotic and biotic stress. We investigated the effect of water stress treatment during 10 days on expression of conserved miRNAs-miR398a/b and miR408 in pea plants. This time frame reflects the changes as close as possible to the changes where water stress causes visible effects under field condition. It was observed that dehydration strongly down regulates the expression of both miR398a/b and miR408 in pea roots and shoots. The down-regulation of miR398a/b and the up-regulation of potential target - genes copper superoxide dismutase, CSD1, highlight the involvement of this miRNA in pea stress response. To the contrary, the mRNA level of cytochrome c oxidase subunit 5 (COX5b) did not change in roots and shoots of water-stressed plants, compared to control (well) hydrated plants. This suggests that COX5b is not the target of miR398, or that its expression is regulated by some other mechanism. P-1B-ATPase expression increased during water deficit only in the shoots of pea; in the roots there were no changes in expression. Our results help to understand the possible role of investigated miRNAs and their contribution to pea capacity to cope with water deficit. PB - Elsevier France-Editions Scientifiques Medicales Elsevier, Paris T2 - Plant Physiology and Biochemistry T1 - Water deficit down-regulates miR398 and miR408 in pea (Pisum sativum L.) EP - 31 SP - 26 VL - 83 DO - 10.1016/j.plaphy.2014.07.008 ER -
@article{ author = "Jovanović, Živko and Stanisavljević, Nemanja and Mikić, Aleksandar and Radović, Svetlana and Maksimović, Vesna R.", year = "2014", abstract = "MicroRNAs (miRNAs), recently recognized as important regulator of gene expression at post-transcriptional level, have been found to be involved in plant stress responses. The observation that some miRNAs are up- or down regulated by stress implies that they could play vital roles in plant resistance to abiotic and biotic stress. We investigated the effect of water stress treatment during 10 days on expression of conserved miRNAs-miR398a/b and miR408 in pea plants. This time frame reflects the changes as close as possible to the changes where water stress causes visible effects under field condition. It was observed that dehydration strongly down regulates the expression of both miR398a/b and miR408 in pea roots and shoots. The down-regulation of miR398a/b and the up-regulation of potential target - genes copper superoxide dismutase, CSD1, highlight the involvement of this miRNA in pea stress response. To the contrary, the mRNA level of cytochrome c oxidase subunit 5 (COX5b) did not change in roots and shoots of water-stressed plants, compared to control (well) hydrated plants. This suggests that COX5b is not the target of miR398, or that its expression is regulated by some other mechanism. P-1B-ATPase expression increased during water deficit only in the shoots of pea; in the roots there were no changes in expression. Our results help to understand the possible role of investigated miRNAs and their contribution to pea capacity to cope with water deficit.", publisher = "Elsevier France-Editions Scientifiques Medicales Elsevier, Paris", journal = "Plant Physiology and Biochemistry", title = "Water deficit down-regulates miR398 and miR408 in pea (Pisum sativum L.)", pages = "31-26", volume = "83", doi = "10.1016/j.plaphy.2014.07.008" }
Jovanović, Ž., Stanisavljević, N., Mikić, A., Radović, S.,& Maksimović, V. R.. (2014). Water deficit down-regulates miR398 and miR408 in pea (Pisum sativum L.). in Plant Physiology and Biochemistry Elsevier France-Editions Scientifiques Medicales Elsevier, Paris., 83, 26-31. https://doi.org/10.1016/j.plaphy.2014.07.008
Jovanović Ž, Stanisavljević N, Mikić A, Radović S, Maksimović VR. Water deficit down-regulates miR398 and miR408 in pea (Pisum sativum L.). in Plant Physiology and Biochemistry. 2014;83:26-31. doi:10.1016/j.plaphy.2014.07.008 .
Jovanović, Živko, Stanisavljević, Nemanja, Mikić, Aleksandar, Radović, Svetlana, Maksimović, Vesna R., "Water deficit down-regulates miR398 and miR408 in pea (Pisum sativum L.)" in Plant Physiology and Biochemistry, 83 (2014):26-31, https://doi.org/10.1016/j.plaphy.2014.07.008 . .