Efficient transformation of Micromonospora melanosporea protoplasts by Streptomyces plasmid

dc.creator	Kojić, Milorad
dc.creator	Topisirović, Ljubiša
dc.creator	Vasiljević, Branka
dc.date.accessioned	2022-11-15T13:27:36Z
dc.date.available	2022-11-15T13:27:36Z
dc.date.issued	1991
dc.identifier.issn	0343-8651
dc.identifier.uri	https://imagine.imgge.bg.ac.rs/handle/123456789/74
dc.description.abstract	An efficient and relatively simple procedure for Micromonospora melanosporea protoplast preparation and transformation is described. Transformation of M. melanosporea protoplast by the Streptomyces plasmid pIJ702 was optimized by altering parameters affecting the formation, regeneration, and transformation of protoplasts. Improvement of regeneration medium resulted in relatively quick growth of transformants (only 7 days). As a result of these experiments we describe a new transformation method that has routinely yielded 106 transformants/μg plasmid DNA.	en
dc.publisher	Springer-Verlag
dc.rights	restrictedAccess
dc.source	Current Microbiology
dc.title	Efficient transformation of Micromonospora melanosporea protoplasts by Streptomyces plasmid	en
dc.type	article
dc.rights.license	ARR
dc.citation.epage	345
dc.citation.issue	6
dc.citation.other	23(6): 343-345
dc.citation.spage	343
dc.citation.volume	23
dc.identifier.doi	10.1007/BF02104137
dc.identifier.scopus	2-s2.0-0026345358
dc.type.version	publishedVersion