Molecular characterization of natural dairy isolates of enterococcus faecalis and evaluation of their antimicrobial potential
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Veljović, KatarinaTerzić-Vidojević, Amarela
Tolinački, Maja
Mihajlović, S.
Vukotić, Goran
Golić, Nataša
Kojić, Milan
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Due to their ability to survive adverse conditions, enterococci are widespread in nature and can be found in milk, dairy products and human and animal gastrointestinal tracts. Still, the use of enterococci in food preparation is controversial, since they have traditionally been branded as indicators of faecal contamination and their role in food spoilage is well known. However, some enterococcal strains exhibit antimicrobial effects and have probiotic potential, contributing to the improvement of the general state of health. For that reason, we have analyzed natural isolates of Enterococcus faecalis originating from various dairy products manufactured in rural households located in the mountains of Serbia. Genotyping analysis of selected enterococci showed high diversity among the isolates. The antimicrobial activity of the isolates showed a great effect on the number of pathogenic and non-pathogenic strains, including L. monocytogenes, L. innocua, E. coli, Pseudomonas sp., and Candida... pseudotropicalis. Furthermore, analysis of the presence of known bacteriocin encoding genes showed that the genes for various enterocins were present. Although in some strains more than one enterocin gene was detected, there was no correlation between the number of enterocin genes and the antimicrobial spectrum. Nevertheless, in order to characterize the strains that could be safely used as starter cultures in functional food, the frequency of virulence determinants and antibiotic resistance, as well as the synthesis of biogenic amines, was analyzed. The results show that the presence of virulence determinants and antibiotic resistance is strain dependent and region specific. In addition, a large percentage of the strains have the ability to decarboxylate tyrosine and other amino acids. Such capacity for decarboxylation of amino acids limits the use of the strains in the food industry. Based on these results, it can be concluded that enterococci isolated from animal food must be viewed with particular caution because they are reservoirs of genes for antibiotic resistance and virulence.
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Enterococcus Faecalis: Molecular Characteristics, Role in Nosocomial Infections and Antibacterial Effects, 2014, 123-135Publisher:
- Nova Science Publishers, Inc.
Scopus: 2-s2.0-84946710067
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Institut za molekularnu genetiku i genetičko inženjerstvoTY - CHAP AU - Veljović, Katarina AU - Terzić-Vidojević, Amarela AU - Tolinački, Maja AU - Mihajlović, S. AU - Vukotić, Goran AU - Golić, Nataša AU - Kojić, Milan PY - 2014 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/762 AB - Due to their ability to survive adverse conditions, enterococci are widespread in nature and can be found in milk, dairy products and human and animal gastrointestinal tracts. Still, the use of enterococci in food preparation is controversial, since they have traditionally been branded as indicators of faecal contamination and their role in food spoilage is well known. However, some enterococcal strains exhibit antimicrobial effects and have probiotic potential, contributing to the improvement of the general state of health. For that reason, we have analyzed natural isolates of Enterococcus faecalis originating from various dairy products manufactured in rural households located in the mountains of Serbia. Genotyping analysis of selected enterococci showed high diversity among the isolates. The antimicrobial activity of the isolates showed a great effect on the number of pathogenic and non-pathogenic strains, including L. monocytogenes, L. innocua, E. coli, Pseudomonas sp., and Candida pseudotropicalis. Furthermore, analysis of the presence of known bacteriocin encoding genes showed that the genes for various enterocins were present. Although in some strains more than one enterocin gene was detected, there was no correlation between the number of enterocin genes and the antimicrobial spectrum. Nevertheless, in order to characterize the strains that could be safely used as starter cultures in functional food, the frequency of virulence determinants and antibiotic resistance, as well as the synthesis of biogenic amines, was analyzed. The results show that the presence of virulence determinants and antibiotic resistance is strain dependent and region specific. In addition, a large percentage of the strains have the ability to decarboxylate tyrosine and other amino acids. Such capacity for decarboxylation of amino acids limits the use of the strains in the food industry. Based on these results, it can be concluded that enterococci isolated from animal food must be viewed with particular caution because they are reservoirs of genes for antibiotic resistance and virulence. PB - Nova Science Publishers, Inc. T2 - Enterococcus Faecalis: Molecular Characteristics, Role in Nosocomial Infections and Antibacterial Effects T1 - Molecular characterization of natural dairy isolates of enterococcus faecalis and evaluation of their antimicrobial potential EP - 135 SP - 123 UR - https://hdl.handle.net/21.15107/rcub_imagine_762 ER -
@inbook{ author = "Veljović, Katarina and Terzić-Vidojević, Amarela and Tolinački, Maja and Mihajlović, S. and Vukotić, Goran and Golić, Nataša and Kojić, Milan", year = "2014", abstract = "Due to their ability to survive adverse conditions, enterococci are widespread in nature and can be found in milk, dairy products and human and animal gastrointestinal tracts. Still, the use of enterococci in food preparation is controversial, since they have traditionally been branded as indicators of faecal contamination and their role in food spoilage is well known. However, some enterococcal strains exhibit antimicrobial effects and have probiotic potential, contributing to the improvement of the general state of health. For that reason, we have analyzed natural isolates of Enterococcus faecalis originating from various dairy products manufactured in rural households located in the mountains of Serbia. Genotyping analysis of selected enterococci showed high diversity among the isolates. The antimicrobial activity of the isolates showed a great effect on the number of pathogenic and non-pathogenic strains, including L. monocytogenes, L. innocua, E. coli, Pseudomonas sp., and Candida pseudotropicalis. Furthermore, analysis of the presence of known bacteriocin encoding genes showed that the genes for various enterocins were present. Although in some strains more than one enterocin gene was detected, there was no correlation between the number of enterocin genes and the antimicrobial spectrum. Nevertheless, in order to characterize the strains that could be safely used as starter cultures in functional food, the frequency of virulence determinants and antibiotic resistance, as well as the synthesis of biogenic amines, was analyzed. The results show that the presence of virulence determinants and antibiotic resistance is strain dependent and region specific. In addition, a large percentage of the strains have the ability to decarboxylate tyrosine and other amino acids. Such capacity for decarboxylation of amino acids limits the use of the strains in the food industry. Based on these results, it can be concluded that enterococci isolated from animal food must be viewed with particular caution because they are reservoirs of genes for antibiotic resistance and virulence.", publisher = "Nova Science Publishers, Inc.", journal = "Enterococcus Faecalis: Molecular Characteristics, Role in Nosocomial Infections and Antibacterial Effects", booktitle = "Molecular characterization of natural dairy isolates of enterococcus faecalis and evaluation of their antimicrobial potential", pages = "135-123", url = "https://hdl.handle.net/21.15107/rcub_imagine_762" }
Veljović, K., Terzić-Vidojević, A., Tolinački, M., Mihajlović, S., Vukotić, G., Golić, N.,& Kojić, M.. (2014). Molecular characterization of natural dairy isolates of enterococcus faecalis and evaluation of their antimicrobial potential. in Enterococcus Faecalis: Molecular Characteristics, Role in Nosocomial Infections and Antibacterial Effects Nova Science Publishers, Inc.., 123-135. https://hdl.handle.net/21.15107/rcub_imagine_762
Veljović K, Terzić-Vidojević A, Tolinački M, Mihajlović S, Vukotić G, Golić N, Kojić M. Molecular characterization of natural dairy isolates of enterococcus faecalis and evaluation of their antimicrobial potential. in Enterococcus Faecalis: Molecular Characteristics, Role in Nosocomial Infections and Antibacterial Effects. 2014;:123-135. https://hdl.handle.net/21.15107/rcub_imagine_762 .
Veljović, Katarina, Terzić-Vidojević, Amarela, Tolinački, Maja, Mihajlović, S., Vukotić, Goran, Golić, Nataša, Kojić, Milan, "Molecular characterization of natural dairy isolates of enterococcus faecalis and evaluation of their antimicrobial potential" in Enterococcus Faecalis: Molecular Characteristics, Role in Nosocomial Infections and Antibacterial Effects (2014):123-135, https://hdl.handle.net/21.15107/rcub_imagine_762 .