Molecular and biochemical characterization of pseudomonas putida isolated from bottled uncarbonated mineral drinking water
Апстракт
Pseudomonas putida belongs to a group of opportunistic pathogens that can cause disease in people with weakened or damaged immune systems. Some strains have medical significance, and for most ingestion is not the primary route of infection. If water used by predisposed subjects is contaminated by P. putida, they may become ill. The aim of this work was the biochemical and molecular characterization of strain ST3 of P. putida isolated from non-carbonated bottled drinking water from Jakov Do 4 on Mt. Vlasina. Characterization of P. putida was performed to assess the risk to human health of the indigenous strains present in the water. Biochemical characterization of strains was performed using the manual identification system ID 32 GN (BioMerieux). Identification was obtained using the database identification software ATB System (Bio-Merieux). Molecular characterization was performed by PCR amplification and 16S rDNA "thermal cycling sequencing". Biochemical identification of the strain S...T3 was accurate (Id = 99.8%). Comparing the sequences obtained for strain ST3 with NCBI gene bank sequences for 16S rRNA, the highest similarity of our strain (96% identity) with a strain of P. putida, designated as biotype A (gi vertical bar 18076625 vertical bar emb vertical bar AJ308311.1 vertical bar.PPU308311) isolated in New Zealand, was obtained. While comparison with the NCBI collection of all deposited sequences showed that the 16S rRNA gene sequence of strain ST3 has very high homology, it is not identical, indicating indirectly that strain ST-3 is an indigenous strain.
Кључне речи:
Pseudomonas putida / drinking water / 16S rRNA geneИзвор:
Archives of Biological Sciences, 2014, 66, 1, 23-28Издавач:
- Srpsko biološko društvo, Beograd, i dr.
DOI: 10.2298/ABS1401023T
ISSN: 0354-4664
WoS: 000330552400004
Scopus: 2-s2.0-84892393662
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Tasić, S. AU - Kojić, Milan AU - Obradović, D. AU - Tasić, Irena PY - 2014 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/765 AB - Pseudomonas putida belongs to a group of opportunistic pathogens that can cause disease in people with weakened or damaged immune systems. Some strains have medical significance, and for most ingestion is not the primary route of infection. If water used by predisposed subjects is contaminated by P. putida, they may become ill. The aim of this work was the biochemical and molecular characterization of strain ST3 of P. putida isolated from non-carbonated bottled drinking water from Jakov Do 4 on Mt. Vlasina. Characterization of P. putida was performed to assess the risk to human health of the indigenous strains present in the water. Biochemical characterization of strains was performed using the manual identification system ID 32 GN (BioMerieux). Identification was obtained using the database identification software ATB System (Bio-Merieux). Molecular characterization was performed by PCR amplification and 16S rDNA "thermal cycling sequencing". Biochemical identification of the strain ST3 was accurate (Id = 99.8%). Comparing the sequences obtained for strain ST3 with NCBI gene bank sequences for 16S rRNA, the highest similarity of our strain (96% identity) with a strain of P. putida, designated as biotype A (gi vertical bar 18076625 vertical bar emb vertical bar AJ308311.1 vertical bar.PPU308311) isolated in New Zealand, was obtained. While comparison with the NCBI collection of all deposited sequences showed that the 16S rRNA gene sequence of strain ST3 has very high homology, it is not identical, indicating indirectly that strain ST-3 is an indigenous strain. PB - Srpsko biološko društvo, Beograd, i dr. T2 - Archives of Biological Sciences T1 - Molecular and biochemical characterization of pseudomonas putida isolated from bottled uncarbonated mineral drinking water EP - 28 IS - 1 SP - 23 VL - 66 DO - 10.2298/ABS1401023T ER -
@article{ author = "Tasić, S. and Kojić, Milan and Obradović, D. and Tasić, Irena", year = "2014", abstract = "Pseudomonas putida belongs to a group of opportunistic pathogens that can cause disease in people with weakened or damaged immune systems. Some strains have medical significance, and for most ingestion is not the primary route of infection. If water used by predisposed subjects is contaminated by P. putida, they may become ill. The aim of this work was the biochemical and molecular characterization of strain ST3 of P. putida isolated from non-carbonated bottled drinking water from Jakov Do 4 on Mt. Vlasina. Characterization of P. putida was performed to assess the risk to human health of the indigenous strains present in the water. Biochemical characterization of strains was performed using the manual identification system ID 32 GN (BioMerieux). Identification was obtained using the database identification software ATB System (Bio-Merieux). Molecular characterization was performed by PCR amplification and 16S rDNA "thermal cycling sequencing". Biochemical identification of the strain ST3 was accurate (Id = 99.8%). Comparing the sequences obtained for strain ST3 with NCBI gene bank sequences for 16S rRNA, the highest similarity of our strain (96% identity) with a strain of P. putida, designated as biotype A (gi vertical bar 18076625 vertical bar emb vertical bar AJ308311.1 vertical bar.PPU308311) isolated in New Zealand, was obtained. While comparison with the NCBI collection of all deposited sequences showed that the 16S rRNA gene sequence of strain ST3 has very high homology, it is not identical, indicating indirectly that strain ST-3 is an indigenous strain.", publisher = "Srpsko biološko društvo, Beograd, i dr.", journal = "Archives of Biological Sciences", title = "Molecular and biochemical characterization of pseudomonas putida isolated from bottled uncarbonated mineral drinking water", pages = "28-23", number = "1", volume = "66", doi = "10.2298/ABS1401023T" }
Tasić, S., Kojić, M., Obradović, D.,& Tasić, I.. (2014). Molecular and biochemical characterization of pseudomonas putida isolated from bottled uncarbonated mineral drinking water. in Archives of Biological Sciences Srpsko biološko društvo, Beograd, i dr.., 66(1), 23-28. https://doi.org/10.2298/ABS1401023T
Tasić S, Kojić M, Obradović D, Tasić I. Molecular and biochemical characterization of pseudomonas putida isolated from bottled uncarbonated mineral drinking water. in Archives of Biological Sciences. 2014;66(1):23-28. doi:10.2298/ABS1401023T .
Tasić, S., Kojić, Milan, Obradović, D., Tasić, Irena, "Molecular and biochemical characterization of pseudomonas putida isolated from bottled uncarbonated mineral drinking water" in Archives of Biological Sciences, 66, no. 1 (2014):23-28, https://doi.org/10.2298/ABS1401023T . .