Hybrid P(L)tl promoter with dual regulation control
Само за регистроване кориснике
1991
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The newly constructed P(L)tl hybrid promoter is composed of the operator and promoter sequences of tac and the -35 to -135 region of the phage λ P(L) promoter, which contains the AT-rich block and the OL2 and OL3 segments. Transcriptional properties of P(L)tl were examined and compared with tac and λ P(L) as reference promoters. The hybrid P(L)tl exhibits different and improved properties over tac promoter in four ways: (i) when repressed, the repression is almost complete; (ii) after complete induction, the hybrid P(L)tl promoter shows a 1.4-2 times higher expression; (iii) the hybrid P(L)tl promoter permits flexible gene expression because it can be utilized under either or both repression controls simultaneously; (iv) the P(L)tl promoter permits enhanced expression of genes encoding products with unknown properties. When compared with the strong promoter P(L) from phage λ, results with the P(L)tl promoter in lacZ fusion constructs show higher levels of β-galactosidase activity. We a...lso constructed a plasmid vector, pP(L)tl7G, which contains the hybrid P(L)tl promoter with a polylinker sequence at its 3' end, which facilitates efficient fusions of foreign genes in any of the reading frame.
Извор:
DNA and Cell Biology, 1991, 10, 5, 389-395
DOI: 10.1089/dna.1991.10.389
ISSN: 1044-5498
PubMed: 1830746
Scopus: 2-s2.0-0025922523
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Konstantinović, M. AU - Maksimović, Vesna R. AU - Nikčević, Gordana AU - Glisin, V. PY - 1991 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/77 AB - The newly constructed P(L)tl hybrid promoter is composed of the operator and promoter sequences of tac and the -35 to -135 region of the phage λ P(L) promoter, which contains the AT-rich block and the OL2 and OL3 segments. Transcriptional properties of P(L)tl were examined and compared with tac and λ P(L) as reference promoters. The hybrid P(L)tl exhibits different and improved properties over tac promoter in four ways: (i) when repressed, the repression is almost complete; (ii) after complete induction, the hybrid P(L)tl promoter shows a 1.4-2 times higher expression; (iii) the hybrid P(L)tl promoter permits flexible gene expression because it can be utilized under either or both repression controls simultaneously; (iv) the P(L)tl promoter permits enhanced expression of genes encoding products with unknown properties. When compared with the strong promoter P(L) from phage λ, results with the P(L)tl promoter in lacZ fusion constructs show higher levels of β-galactosidase activity. We also constructed a plasmid vector, pP(L)tl7G, which contains the hybrid P(L)tl promoter with a polylinker sequence at its 3' end, which facilitates efficient fusions of foreign genes in any of the reading frame. T2 - DNA and Cell Biology T1 - Hybrid P(L)tl promoter with dual regulation control EP - 395 IS - 5 SP - 389 VL - 10 DO - 10.1089/dna.1991.10.389 ER -
@article{ author = "Konstantinović, M. and Maksimović, Vesna R. and Nikčević, Gordana and Glisin, V.", year = "1991", abstract = "The newly constructed P(L)tl hybrid promoter is composed of the operator and promoter sequences of tac and the -35 to -135 region of the phage λ P(L) promoter, which contains the AT-rich block and the OL2 and OL3 segments. Transcriptional properties of P(L)tl were examined and compared with tac and λ P(L) as reference promoters. The hybrid P(L)tl exhibits different and improved properties over tac promoter in four ways: (i) when repressed, the repression is almost complete; (ii) after complete induction, the hybrid P(L)tl promoter shows a 1.4-2 times higher expression; (iii) the hybrid P(L)tl promoter permits flexible gene expression because it can be utilized under either or both repression controls simultaneously; (iv) the P(L)tl promoter permits enhanced expression of genes encoding products with unknown properties. When compared with the strong promoter P(L) from phage λ, results with the P(L)tl promoter in lacZ fusion constructs show higher levels of β-galactosidase activity. We also constructed a plasmid vector, pP(L)tl7G, which contains the hybrid P(L)tl promoter with a polylinker sequence at its 3' end, which facilitates efficient fusions of foreign genes in any of the reading frame.", journal = "DNA and Cell Biology", title = "Hybrid P(L)tl promoter with dual regulation control", pages = "395-389", number = "5", volume = "10", doi = "10.1089/dna.1991.10.389" }
Konstantinović, M., Maksimović, V. R., Nikčević, G.,& Glisin, V.. (1991). Hybrid P(L)tl promoter with dual regulation control. in DNA and Cell Biology, 10(5), 389-395. https://doi.org/10.1089/dna.1991.10.389
Konstantinović M, Maksimović VR, Nikčević G, Glisin V. Hybrid P(L)tl promoter with dual regulation control. in DNA and Cell Biology. 1991;10(5):389-395. doi:10.1089/dna.1991.10.389 .
Konstantinović, M., Maksimović, Vesna R., Nikčević, Gordana, Glisin, V., "Hybrid P(L)tl promoter with dual regulation control" in DNA and Cell Biology, 10, no. 5 (1991):389-395, https://doi.org/10.1089/dna.1991.10.389 . .