Telomere-associated chromosome fragmentation: Applications in genome manipulation and analysis
Само за регистроване кориснике
1992
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Telomere-associated chromosome fragmentation (TACF) is a new approach for chromosome mapping based on the non-targeted introduction of cloned telomeres into mammalian cells. TACF has been used to generate a panel of somatic cell hybrids with nested terminal deletions of the long arm of the human × chromosome, extending from Xq26 to the centromere. This panel has been characterized using a series of × chromosome loci. Recovery of the end clones by plasmid rescue produces a telomeric marker for each cell line and partial sequencing will allow the generation of sequence tagged sites (STSs). TACF provides a powerful and widely applicable method for genome analysis, a general way of manipulating mammalian chromosomes and a first step towards constructing artificial mammalian chromosomes.
Извор:
Nature Genetics, 1992, 2, 4, 275-282
DOI: 10.1038/ng1292-275
ISSN: 1061-4036
PubMed: 1303279
Scopus: 2-s2.0-0027034002
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Farr, C.J. AU - Stevanović, Milena AU - Thomson, E.J. AU - Goodfellow, P.N. AU - Cooke, H.J. PY - 1992 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/79 AB - Telomere-associated chromosome fragmentation (TACF) is a new approach for chromosome mapping based on the non-targeted introduction of cloned telomeres into mammalian cells. TACF has been used to generate a panel of somatic cell hybrids with nested terminal deletions of the long arm of the human × chromosome, extending from Xq26 to the centromere. This panel has been characterized using a series of × chromosome loci. Recovery of the end clones by plasmid rescue produces a telomeric marker for each cell line and partial sequencing will allow the generation of sequence tagged sites (STSs). TACF provides a powerful and widely applicable method for genome analysis, a general way of manipulating mammalian chromosomes and a first step towards constructing artificial mammalian chromosomes. T2 - Nature Genetics T1 - Telomere-associated chromosome fragmentation: Applications in genome manipulation and analysis EP - 282 IS - 4 SP - 275 VL - 2 DO - 10.1038/ng1292-275 ER -
@article{ author = "Farr, C.J. and Stevanović, Milena and Thomson, E.J. and Goodfellow, P.N. and Cooke, H.J.", year = "1992", abstract = "Telomere-associated chromosome fragmentation (TACF) is a new approach for chromosome mapping based on the non-targeted introduction of cloned telomeres into mammalian cells. TACF has been used to generate a panel of somatic cell hybrids with nested terminal deletions of the long arm of the human × chromosome, extending from Xq26 to the centromere. This panel has been characterized using a series of × chromosome loci. Recovery of the end clones by plasmid rescue produces a telomeric marker for each cell line and partial sequencing will allow the generation of sequence tagged sites (STSs). TACF provides a powerful and widely applicable method for genome analysis, a general way of manipulating mammalian chromosomes and a first step towards constructing artificial mammalian chromosomes.", journal = "Nature Genetics", title = "Telomere-associated chromosome fragmentation: Applications in genome manipulation and analysis", pages = "282-275", number = "4", volume = "2", doi = "10.1038/ng1292-275" }
Farr, C.J., Stevanović, M., Thomson, E.J., Goodfellow, P.N.,& Cooke, H.J.. (1992). Telomere-associated chromosome fragmentation: Applications in genome manipulation and analysis. in Nature Genetics, 2(4), 275-282. https://doi.org/10.1038/ng1292-275
Farr C, Stevanović M, Thomson E, Goodfellow P, Cooke H. Telomere-associated chromosome fragmentation: Applications in genome manipulation and analysis. in Nature Genetics. 1992;2(4):275-282. doi:10.1038/ng1292-275 .
Farr, C.J., Stevanović, Milena, Thomson, E.J., Goodfellow, P.N., Cooke, H.J., "Telomere-associated chromosome fragmentation: Applications in genome manipulation and analysis" in Nature Genetics, 2, no. 4 (1992):275-282, https://doi.org/10.1038/ng1292-275 . .