Cloning and characterization of an aminoglycoside resistance determinant from Micromonospora zionensis
Apstrakt
The sisomicin-gentamicin resistance methylase (sgm) gene was isolated from Micromonospora zionensis and cloned in Streptomyces lividans. The sgm gene was expressed in Micromonospora melanosporea, where its own promoter was active, and also in Escherichia coli under the control of the lacZ promoter. The complete nucleotide sequence of 1,122 bp and a transcription start point were determined. The sequence contains an open reading frame that encodes a polypeptide of 274 amino acids. The methylation of 30S ribosomal subunits by Sgm methylase accounts adequately for all known resistance characteristics of M. zionensis, but expression of high-level resistance to hygromycin B is background dependent. A comparison of the amino acid sequence of the predicted Sgm protein with the deduced amino acid sequences for the 16S rRNA methylases showed extensive similarity to Grm and significant similarity to KgmB but not to KamB methylase.
Izvor:
Journal of Bacteriology, 1992, 174, 23, 7868-7872
DOI: 10.1128/jb.174.23.7868-7872.1992
ISSN: 0021-9193
PubMed: 1447159
Scopus: 2-s2.0-0026471641
Institucija/grupa
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Kojić, Milorad AU - Topisirović, Ljubiša AU - Vasiljević, Branka PY - 1992 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/80 AB - The sisomicin-gentamicin resistance methylase (sgm) gene was isolated from Micromonospora zionensis and cloned in Streptomyces lividans. The sgm gene was expressed in Micromonospora melanosporea, where its own promoter was active, and also in Escherichia coli under the control of the lacZ promoter. The complete nucleotide sequence of 1,122 bp and a transcription start point were determined. The sequence contains an open reading frame that encodes a polypeptide of 274 amino acids. The methylation of 30S ribosomal subunits by Sgm methylase accounts adequately for all known resistance characteristics of M. zionensis, but expression of high-level resistance to hygromycin B is background dependent. A comparison of the amino acid sequence of the predicted Sgm protein with the deduced amino acid sequences for the 16S rRNA methylases showed extensive similarity to Grm and significant similarity to KgmB but not to KamB methylase. T2 - Journal of Bacteriology T1 - Cloning and characterization of an aminoglycoside resistance determinant from Micromonospora zionensis EP - 7872 IS - 23 SP - 7868 VL - 174 DO - 10.1128/jb.174.23.7868-7872.1992 ER -
@article{ author = "Kojić, Milorad and Topisirović, Ljubiša and Vasiljević, Branka", year = "1992", abstract = "The sisomicin-gentamicin resistance methylase (sgm) gene was isolated from Micromonospora zionensis and cloned in Streptomyces lividans. The sgm gene was expressed in Micromonospora melanosporea, where its own promoter was active, and also in Escherichia coli under the control of the lacZ promoter. The complete nucleotide sequence of 1,122 bp and a transcription start point were determined. The sequence contains an open reading frame that encodes a polypeptide of 274 amino acids. The methylation of 30S ribosomal subunits by Sgm methylase accounts adequately for all known resistance characteristics of M. zionensis, but expression of high-level resistance to hygromycin B is background dependent. A comparison of the amino acid sequence of the predicted Sgm protein with the deduced amino acid sequences for the 16S rRNA methylases showed extensive similarity to Grm and significant similarity to KgmB but not to KamB methylase.", journal = "Journal of Bacteriology", title = "Cloning and characterization of an aminoglycoside resistance determinant from Micromonospora zionensis", pages = "7872-7868", number = "23", volume = "174", doi = "10.1128/jb.174.23.7868-7872.1992" }
Kojić, M., Topisirović, L.,& Vasiljević, B.. (1992). Cloning and characterization of an aminoglycoside resistance determinant from Micromonospora zionensis. in Journal of Bacteriology, 174(23), 7868-7872. https://doi.org/10.1128/jb.174.23.7868-7872.1992
Kojić M, Topisirović L, Vasiljević B. Cloning and characterization of an aminoglycoside resistance determinant from Micromonospora zionensis. in Journal of Bacteriology. 1992;174(23):7868-7872. doi:10.1128/jb.174.23.7868-7872.1992 .
Kojić, Milorad , Topisirović, Ljubiša, Vasiljević, Branka, "Cloning and characterization of an aminoglycoside resistance determinant from Micromonospora zionensis" in Journal of Bacteriology, 174, no. 23 (1992):7868-7872, https://doi.org/10.1128/jb.174.23.7868-7872.1992 . .