Human Embryonal Carcinoma Cells in Serum-free Conditions as an In Vitro Model System of Neural Differentiation
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2015
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Serum is generally regarded as an essential component of many eukaryotic cell culture media, despite the fact that serum composition varies greatly and may be the source of a wide range of artefacts. The objective of this study was to assess serum-free growth conditions for the human embryonal carcinoma cell line, NT2/D1. These cells greatly resemble embryonic stem cells. In the presence of retinoic acid (RA), NT2/D1 cells irreversibly differentiate along the neuronal lineage. We have previously shown that the early phases of neural induction of these cells by RA involve the up-regulation of SOX3 gene expression. Our goal was to compare RA-induced differentiation of NT2/D1 cells in serum-containing and serum-free media, by using SOX3 protein levels as a marker of differentiation. We found that NT2/D1 cells can be successfully grown under serum-free conditions, and that the presence or absence of serum does not affect the level of SOX3 protein after a 48-hour RA induction. However, six ...days of RA treatment resulted in a marked increase in SOX3 protein levels in serum-free media compared to serum-containing media, indicating that serum might have an inhibitory effect on the expression of this neural differentiation marker. This finding is important for both basic and translational studies that hope to exploit cell culture conditions that are free of animal-derived products.
Ključne reči:
SOX3 gene / retinoic acid / NT2/D1 cells / neural differentiation / animal-free cell culture modelIzvor:
ATLA-Alternatives To Laboratory Animals, 2015, 43, 1, 9-18Izdavač:
- Sage Publications Ltd, London
Finansiranje / projekti:
- Proučavanje signalnih puteva i epigenetičkih mehanizama uključenih u kontrolu ekspresije humanih SOX gena: dalje rasvetljavanje njihove uloge u određivanju sudbine i diferencijaciji ćelija (RS-MESTD-Basic Research (BR or ON)-173051)
DOI: 10.1177/026119291504300105
ISSN: 0261-1929
PubMed: 25802994
WoS: 000355911300004
Scopus: 2-s2.0-84929939533
Institucija/grupa
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Jasnić, Jovana AU - Lazić, Andrijana AU - Milivojević, Milena AU - Mojsin, Marija AU - Nikčević, Gordana PY - 2015 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/807 AB - Serum is generally regarded as an essential component of many eukaryotic cell culture media, despite the fact that serum composition varies greatly and may be the source of a wide range of artefacts. The objective of this study was to assess serum-free growth conditions for the human embryonal carcinoma cell line, NT2/D1. These cells greatly resemble embryonic stem cells. In the presence of retinoic acid (RA), NT2/D1 cells irreversibly differentiate along the neuronal lineage. We have previously shown that the early phases of neural induction of these cells by RA involve the up-regulation of SOX3 gene expression. Our goal was to compare RA-induced differentiation of NT2/D1 cells in serum-containing and serum-free media, by using SOX3 protein levels as a marker of differentiation. We found that NT2/D1 cells can be successfully grown under serum-free conditions, and that the presence or absence of serum does not affect the level of SOX3 protein after a 48-hour RA induction. However, six days of RA treatment resulted in a marked increase in SOX3 protein levels in serum-free media compared to serum-containing media, indicating that serum might have an inhibitory effect on the expression of this neural differentiation marker. This finding is important for both basic and translational studies that hope to exploit cell culture conditions that are free of animal-derived products. PB - Sage Publications Ltd, London T2 - ATLA-Alternatives To Laboratory Animals T1 - Human Embryonal Carcinoma Cells in Serum-free Conditions as an In Vitro Model System of Neural Differentiation EP - 18 IS - 1 SP - 9 VL - 43 DO - 10.1177/026119291504300105 ER -
@article{ author = "Jasnić, Jovana and Lazić, Andrijana and Milivojević, Milena and Mojsin, Marija and Nikčević, Gordana", year = "2015", abstract = "Serum is generally regarded as an essential component of many eukaryotic cell culture media, despite the fact that serum composition varies greatly and may be the source of a wide range of artefacts. The objective of this study was to assess serum-free growth conditions for the human embryonal carcinoma cell line, NT2/D1. These cells greatly resemble embryonic stem cells. In the presence of retinoic acid (RA), NT2/D1 cells irreversibly differentiate along the neuronal lineage. We have previously shown that the early phases of neural induction of these cells by RA involve the up-regulation of SOX3 gene expression. Our goal was to compare RA-induced differentiation of NT2/D1 cells in serum-containing and serum-free media, by using SOX3 protein levels as a marker of differentiation. We found that NT2/D1 cells can be successfully grown under serum-free conditions, and that the presence or absence of serum does not affect the level of SOX3 protein after a 48-hour RA induction. However, six days of RA treatment resulted in a marked increase in SOX3 protein levels in serum-free media compared to serum-containing media, indicating that serum might have an inhibitory effect on the expression of this neural differentiation marker. This finding is important for both basic and translational studies that hope to exploit cell culture conditions that are free of animal-derived products.", publisher = "Sage Publications Ltd, London", journal = "ATLA-Alternatives To Laboratory Animals", title = "Human Embryonal Carcinoma Cells in Serum-free Conditions as an In Vitro Model System of Neural Differentiation", pages = "18-9", number = "1", volume = "43", doi = "10.1177/026119291504300105" }
Jasnić, J., Lazić, A., Milivojević, M., Mojsin, M.,& Nikčević, G.. (2015). Human Embryonal Carcinoma Cells in Serum-free Conditions as an In Vitro Model System of Neural Differentiation. in ATLA-Alternatives To Laboratory Animals Sage Publications Ltd, London., 43(1), 9-18. https://doi.org/10.1177/026119291504300105
Jasnić J, Lazić A, Milivojević M, Mojsin M, Nikčević G. Human Embryonal Carcinoma Cells in Serum-free Conditions as an In Vitro Model System of Neural Differentiation. in ATLA-Alternatives To Laboratory Animals. 2015;43(1):9-18. doi:10.1177/026119291504300105 .
Jasnić, Jovana, Lazić, Andrijana, Milivojević, Milena, Mojsin, Marija, Nikčević, Gordana, "Human Embryonal Carcinoma Cells in Serum-free Conditions as an In Vitro Model System of Neural Differentiation" in ATLA-Alternatives To Laboratory Animals, 43, no. 1 (2015):9-18, https://doi.org/10.1177/026119291504300105 . .