Development of kinetic model for testing antifungal effect of Thymus vulgaris L. and Cinnamomum cassia L. essential oils on Aspergillus flavus spores and application for optimization of synergistic effect
Само за регистроване кориснике
2015
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The antifungal effect of essential oils (EOs) of Thymus vulgaris L. (EOT.vulgaris) and Cinnamomum cassia L. (EOC.cassia) against Aspergillus flavus spores was evaluated by determining minimum inhibitory concentration, minimum fungicidal concentrations and fungicidal kinetics. Kinetic model of fungicidal activity of individual EOT.vulgaris and EOC.cassia was developed and its parameters were used to make EO mixture with optimal ratio of individual EOs. Synergism and speed of fungicidal effect of EO mixtures against A. flavus spores were evaluated. Kinetic model revealed optimal ratio EOT.vulgaris: EOC.cassia as 14:1 in mixture. Observed result was validated by comparing fungicidal effect of this mixture to commonly used ratio 1:1 and intermediate 7:1 EO mixtures. All three mixtures showed partial fungicidal synergism, but the time point of fungicidal effect was different. The fastest fungicidal effect was observed in 14:1 EO mixture (after 90 mm), followed by 7:1 (110 min) and 1:1 (180 ...min). The difference in the speed of fungicidal effect could not be detected using standard microdilution susceptibility tests, which demonstrated the importance and usefulness of developed kinetic model for further investigations.
Кључне речи:
Synergism / Kinetic model / Cinnamomum cassia / Aspergillus flavus / Antifungal effectИзвор:
Biochemical Engineering Journal, 2015, 99, 131-137Издавач:
- Elsevier Science Bv, Amsterdam
Финансирање / пројекти:
- Значај доказивања раних лабораторијских биомаркера за исход инвазивних гљивичних инфекција код нас (RS-MESTD-Basic Research (BR or ON)-175034)
DOI: 10.1016/j.bej.2015.03.024
ISSN: 1369-703X
WoS: 000355349900016
Scopus: 2-s2.0-84926135756
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Pekmezović, M. AU - Rajković, K. AU - Barac, A. AU - Šenerović, Lidija AU - Arsić-Arsenijević, Valentina PY - 2015 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/809 AB - The antifungal effect of essential oils (EOs) of Thymus vulgaris L. (EOT.vulgaris) and Cinnamomum cassia L. (EOC.cassia) against Aspergillus flavus spores was evaluated by determining minimum inhibitory concentration, minimum fungicidal concentrations and fungicidal kinetics. Kinetic model of fungicidal activity of individual EOT.vulgaris and EOC.cassia was developed and its parameters were used to make EO mixture with optimal ratio of individual EOs. Synergism and speed of fungicidal effect of EO mixtures against A. flavus spores were evaluated. Kinetic model revealed optimal ratio EOT.vulgaris: EOC.cassia as 14:1 in mixture. Observed result was validated by comparing fungicidal effect of this mixture to commonly used ratio 1:1 and intermediate 7:1 EO mixtures. All three mixtures showed partial fungicidal synergism, but the time point of fungicidal effect was different. The fastest fungicidal effect was observed in 14:1 EO mixture (after 90 mm), followed by 7:1 (110 min) and 1:1 (180 min). The difference in the speed of fungicidal effect could not be detected using standard microdilution susceptibility tests, which demonstrated the importance and usefulness of developed kinetic model for further investigations. PB - Elsevier Science Bv, Amsterdam T2 - Biochemical Engineering Journal T1 - Development of kinetic model for testing antifungal effect of Thymus vulgaris L. and Cinnamomum cassia L. essential oils on Aspergillus flavus spores and application for optimization of synergistic effect EP - 137 SP - 131 VL - 99 DO - 10.1016/j.bej.2015.03.024 ER -
@article{ author = "Pekmezović, M. and Rajković, K. and Barac, A. and Šenerović, Lidija and Arsić-Arsenijević, Valentina", year = "2015", abstract = "The antifungal effect of essential oils (EOs) of Thymus vulgaris L. (EOT.vulgaris) and Cinnamomum cassia L. (EOC.cassia) against Aspergillus flavus spores was evaluated by determining minimum inhibitory concentration, minimum fungicidal concentrations and fungicidal kinetics. Kinetic model of fungicidal activity of individual EOT.vulgaris and EOC.cassia was developed and its parameters were used to make EO mixture with optimal ratio of individual EOs. Synergism and speed of fungicidal effect of EO mixtures against A. flavus spores were evaluated. Kinetic model revealed optimal ratio EOT.vulgaris: EOC.cassia as 14:1 in mixture. Observed result was validated by comparing fungicidal effect of this mixture to commonly used ratio 1:1 and intermediate 7:1 EO mixtures. All three mixtures showed partial fungicidal synergism, but the time point of fungicidal effect was different. The fastest fungicidal effect was observed in 14:1 EO mixture (after 90 mm), followed by 7:1 (110 min) and 1:1 (180 min). The difference in the speed of fungicidal effect could not be detected using standard microdilution susceptibility tests, which demonstrated the importance and usefulness of developed kinetic model for further investigations.", publisher = "Elsevier Science Bv, Amsterdam", journal = "Biochemical Engineering Journal", title = "Development of kinetic model for testing antifungal effect of Thymus vulgaris L. and Cinnamomum cassia L. essential oils on Aspergillus flavus spores and application for optimization of synergistic effect", pages = "137-131", volume = "99", doi = "10.1016/j.bej.2015.03.024" }
Pekmezović, M., Rajković, K., Barac, A., Šenerović, L.,& Arsić-Arsenijević, V.. (2015). Development of kinetic model for testing antifungal effect of Thymus vulgaris L. and Cinnamomum cassia L. essential oils on Aspergillus flavus spores and application for optimization of synergistic effect. in Biochemical Engineering Journal Elsevier Science Bv, Amsterdam., 99, 131-137. https://doi.org/10.1016/j.bej.2015.03.024
Pekmezović M, Rajković K, Barac A, Šenerović L, Arsić-Arsenijević V. Development of kinetic model for testing antifungal effect of Thymus vulgaris L. and Cinnamomum cassia L. essential oils on Aspergillus flavus spores and application for optimization of synergistic effect. in Biochemical Engineering Journal. 2015;99:131-137. doi:10.1016/j.bej.2015.03.024 .
Pekmezović, M., Rajković, K., Barac, A., Šenerović, Lidija, Arsić-Arsenijević, Valentina, "Development of kinetic model for testing antifungal effect of Thymus vulgaris L. and Cinnamomum cassia L. essential oils on Aspergillus flavus spores and application for optimization of synergistic effect" in Biochemical Engineering Journal, 99 (2015):131-137, https://doi.org/10.1016/j.bej.2015.03.024 . .