Micro RNA-155 participates in re-activation of encephalitogenic T cells
2015
Аутори
Jevtić, BojanTimotijević, Gordana
Stanisavljević, Suzana
Momcilović, Miljana
Mostarica-Stojković, Marija
Miljković, Djordje
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
MicroRNAs (miR) are small non-coding RNAs involved in the immune response regulation. miR-155 has been attributed a major pro-inflammatory role in the pathogenesis of multiple sclerosis and its animal model experimental autoimmune encephalomyelitis (EAE). Here, a role of miR-155 in re-activation of encephalitogenic CD4(+) T cells was investigated. Dark Agouti rats were immunized with myelin basic protein (MBP) emulsified in complete Freund's adjuvant. CD4(+) T cells were purified from draining lymph node cells (DLNC) obtained in the inductive phase and from spinal cord immune cells (SCIC) isolated at the peak of EAE. CD4(+) T cells obtained from SCIC (i.e., in vivo re-activated cells) had markedly higher expression of miR-155 in comparison to those purified from DLNC (not re-activated). Likewise, in vitro re-activation of DLNC with MBP led to increase in miR-155 expression. Further, DLNC and DLNC CD4(+) T cells were transfected with an inhibitor of miR-155 during in vitro re-activation.... As a result, expression of important CD4(+) T cell effector cytokines IFN-gamma and IL-17, but not of regulatory cytokines IL-10 and TGF-beta, was reduced. These results imply that miR-155 supports re-activation of encephalitogenic CD4+ T cells. Our results contribute to a view that miR-155 might be a valuable target in multiple sclerosis therapy.
Кључне речи:
micro RNA / Cytokines / Central nervous system / AutoimmunityИзвор:
Biomedicine & Pharmacotherapy, 2015, 74, 206-210Издавач:
- Elsevier France-Editions Scientifiques Medicales Elsevier, Paris
Финансирање / пројекти:
- Ћелијски и молекулски механизми опоравка пацова од експерименталног аутоимунског енцефаломијелитиса (RS-MESTD-Basic Research (BR or ON)-173035)
- Имунопатогенетски и регулаторни механизми у аутоимунским болестима и хроничној инфламацији (RS-MESTD-Basic Research (BR or ON)-175038)
- Молекуларни механизми физиолошке и фармаколошке контроле инфламације и канцера (RS-MESTD-Basic Research (BR or ON)-173013)
DOI: 10.1016/j.biopha.2015.08.011
ISSN: 0753-3322
PubMed: 26349986
WoS: 000361182600031
Scopus: 2-s2.0-84940838745
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Jevtić, Bojan AU - Timotijević, Gordana AU - Stanisavljević, Suzana AU - Momcilović, Miljana AU - Mostarica-Stojković, Marija AU - Miljković, Djordje PY - 2015 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/821 AB - MicroRNAs (miR) are small non-coding RNAs involved in the immune response regulation. miR-155 has been attributed a major pro-inflammatory role in the pathogenesis of multiple sclerosis and its animal model experimental autoimmune encephalomyelitis (EAE). Here, a role of miR-155 in re-activation of encephalitogenic CD4(+) T cells was investigated. Dark Agouti rats were immunized with myelin basic protein (MBP) emulsified in complete Freund's adjuvant. CD4(+) T cells were purified from draining lymph node cells (DLNC) obtained in the inductive phase and from spinal cord immune cells (SCIC) isolated at the peak of EAE. CD4(+) T cells obtained from SCIC (i.e., in vivo re-activated cells) had markedly higher expression of miR-155 in comparison to those purified from DLNC (not re-activated). Likewise, in vitro re-activation of DLNC with MBP led to increase in miR-155 expression. Further, DLNC and DLNC CD4(+) T cells were transfected with an inhibitor of miR-155 during in vitro re-activation. As a result, expression of important CD4(+) T cell effector cytokines IFN-gamma and IL-17, but not of regulatory cytokines IL-10 and TGF-beta, was reduced. These results imply that miR-155 supports re-activation of encephalitogenic CD4+ T cells. Our results contribute to a view that miR-155 might be a valuable target in multiple sclerosis therapy. PB - Elsevier France-Editions Scientifiques Medicales Elsevier, Paris T2 - Biomedicine & Pharmacotherapy T1 - Micro RNA-155 participates in re-activation of encephalitogenic T cells EP - 210 SP - 206 VL - 74 DO - 10.1016/j.biopha.2015.08.011 ER -
@article{ author = "Jevtić, Bojan and Timotijević, Gordana and Stanisavljević, Suzana and Momcilović, Miljana and Mostarica-Stojković, Marija and Miljković, Djordje", year = "2015", abstract = "MicroRNAs (miR) are small non-coding RNAs involved in the immune response regulation. miR-155 has been attributed a major pro-inflammatory role in the pathogenesis of multiple sclerosis and its animal model experimental autoimmune encephalomyelitis (EAE). Here, a role of miR-155 in re-activation of encephalitogenic CD4(+) T cells was investigated. Dark Agouti rats were immunized with myelin basic protein (MBP) emulsified in complete Freund's adjuvant. CD4(+) T cells were purified from draining lymph node cells (DLNC) obtained in the inductive phase and from spinal cord immune cells (SCIC) isolated at the peak of EAE. CD4(+) T cells obtained from SCIC (i.e., in vivo re-activated cells) had markedly higher expression of miR-155 in comparison to those purified from DLNC (not re-activated). Likewise, in vitro re-activation of DLNC with MBP led to increase in miR-155 expression. Further, DLNC and DLNC CD4(+) T cells were transfected with an inhibitor of miR-155 during in vitro re-activation. As a result, expression of important CD4(+) T cell effector cytokines IFN-gamma and IL-17, but not of regulatory cytokines IL-10 and TGF-beta, was reduced. These results imply that miR-155 supports re-activation of encephalitogenic CD4+ T cells. Our results contribute to a view that miR-155 might be a valuable target in multiple sclerosis therapy.", publisher = "Elsevier France-Editions Scientifiques Medicales Elsevier, Paris", journal = "Biomedicine & Pharmacotherapy", title = "Micro RNA-155 participates in re-activation of encephalitogenic T cells", pages = "210-206", volume = "74", doi = "10.1016/j.biopha.2015.08.011" }
Jevtić, B., Timotijević, G., Stanisavljević, S., Momcilović, M., Mostarica-Stojković, M.,& Miljković, D.. (2015). Micro RNA-155 participates in re-activation of encephalitogenic T cells. in Biomedicine & Pharmacotherapy Elsevier France-Editions Scientifiques Medicales Elsevier, Paris., 74, 206-210. https://doi.org/10.1016/j.biopha.2015.08.011
Jevtić B, Timotijević G, Stanisavljević S, Momcilović M, Mostarica-Stojković M, Miljković D. Micro RNA-155 participates in re-activation of encephalitogenic T cells. in Biomedicine & Pharmacotherapy. 2015;74:206-210. doi:10.1016/j.biopha.2015.08.011 .
Jevtić, Bojan, Timotijević, Gordana, Stanisavljević, Suzana, Momcilović, Miljana, Mostarica-Stojković, Marija, Miljković, Djordje, "Micro RNA-155 participates in re-activation of encephalitogenic T cells" in Biomedicine & Pharmacotherapy, 74 (2015):206-210, https://doi.org/10.1016/j.biopha.2015.08.011 . .