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Micro RNA-155 participates in re-activation of encephalitogenic T cells
dc.creator | Jevtić, Bojan | |
dc.creator | Timotijević, Gordana | |
dc.creator | Stanisavljević, Suzana | |
dc.creator | Momcilović, Miljana | |
dc.creator | Mostarica-Stojković, Marija | |
dc.creator | Miljković, Djordje | |
dc.date.accessioned | 2022-11-15T14:33:57Z | |
dc.date.available | 2022-11-15T14:33:57Z | |
dc.date.issued | 2015 | |
dc.identifier.issn | 0753-3322 | |
dc.identifier.uri | https://imagine.imgge.bg.ac.rs/handle/123456789/821 | |
dc.description.abstract | MicroRNAs (miR) are small non-coding RNAs involved in the immune response regulation. miR-155 has been attributed a major pro-inflammatory role in the pathogenesis of multiple sclerosis and its animal model experimental autoimmune encephalomyelitis (EAE). Here, a role of miR-155 in re-activation of encephalitogenic CD4(+) T cells was investigated. Dark Agouti rats were immunized with myelin basic protein (MBP) emulsified in complete Freund's adjuvant. CD4(+) T cells were purified from draining lymph node cells (DLNC) obtained in the inductive phase and from spinal cord immune cells (SCIC) isolated at the peak of EAE. CD4(+) T cells obtained from SCIC (i.e., in vivo re-activated cells) had markedly higher expression of miR-155 in comparison to those purified from DLNC (not re-activated). Likewise, in vitro re-activation of DLNC with MBP led to increase in miR-155 expression. Further, DLNC and DLNC CD4(+) T cells were transfected with an inhibitor of miR-155 during in vitro re-activation. As a result, expression of important CD4(+) T cell effector cytokines IFN-gamma and IL-17, but not of regulatory cytokines IL-10 and TGF-beta, was reduced. These results imply that miR-155 supports re-activation of encephalitogenic CD4+ T cells. Our results contribute to a view that miR-155 might be a valuable target in multiple sclerosis therapy. | en |
dc.publisher | Elsevier France-Editions Scientifiques Medicales Elsevier, Paris | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173035/RS// | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175038/RS// | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173013/RS// | |
dc.rights | openAccess | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.source | Biomedicine & Pharmacotherapy | |
dc.subject | micro RNA | en |
dc.subject | Cytokines | en |
dc.subject | Central nervous system | en |
dc.subject | Autoimmunity | en |
dc.title | Micro RNA-155 participates in re-activation of encephalitogenic T cells | en |
dc.type | article | |
dc.rights.license | BY | |
dc.citation.epage | 210 | |
dc.citation.other | 74(): 206-210 | |
dc.citation.rank | M22 | |
dc.citation.spage | 206 | |
dc.citation.volume | 74 | |
dc.identifier.doi | 10.1016/j.biopha.2015.08.011 | |
dc.identifier.fulltext | https://imagine.imgge.bg.ac.rs/bitstream/id/739/818.pdf | |
dc.identifier.pmid | 26349986 | |
dc.identifier.scopus | 2-s2.0-84940838745 | |
dc.identifier.wos | 000361182600031 | |
dc.type.version | publishedVersion |