Constitutive interferon expression from retroviral vector
Abstract
A genomic fragment with the human β-interferon gene was cloned into a pL3-4, a defective Moloney murine leukemia virus (M-MuLV) vector. Here we show that clones selected after viral infection of mouse NIH 3T3 cells constitutively produced 128 IU/ml of human β-interferon. Constitutive synthesis of retroviral RNA was confirmed by dot blot hybridization of RNA isolated from two of the selected clones. Poly(I)·poly(C) and cycloheximide induction resulted in an increased RNA level, but this was not reflected in an increased production of biologically active interferon.
Keywords:
Retrovirus / Interferon / Constitutive expressionSource:
Antiviral Research, 1993, 22, 2-3, 215-221Funding / projects:
- We thank Dr. E.F. Wagner for providing Psi-2 and NIH 3T3 cell lines and pL3-4 vector, Dr. R. Falcoff for WISH cells and Dr. Konstantinovic for pIF plasmid. This research was supported by the Serbian Research Fund Grant 1.41 and 1CGEB-UNIDO, CRP/YUG 90-04.
DOI: 10.1016/0166-3542(93)90097-3
ISSN: 0166-3542
PubMed: 8279813
Scopus: 2-s2.0-0027501128
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Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Knezić, Z. AU - Nikčević, Gordana AU - Marjanović, J. AU - Glisin, V. AU - Popović, Z. PY - 1993 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/83 AB - A genomic fragment with the human β-interferon gene was cloned into a pL3-4, a defective Moloney murine leukemia virus (M-MuLV) vector. Here we show that clones selected after viral infection of mouse NIH 3T3 cells constitutively produced 128 IU/ml of human β-interferon. Constitutive synthesis of retroviral RNA was confirmed by dot blot hybridization of RNA isolated from two of the selected clones. Poly(I)·poly(C) and cycloheximide induction resulted in an increased RNA level, but this was not reflected in an increased production of biologically active interferon. T2 - Antiviral Research T1 - Constitutive interferon expression from retroviral vector EP - 221 IS - 2-3 SP - 215 VL - 22 DO - 10.1016/0166-3542(93)90097-3 ER -
@article{ author = "Knezić, Z. and Nikčević, Gordana and Marjanović, J. and Glisin, V. and Popović, Z.", year = "1993", abstract = "A genomic fragment with the human β-interferon gene was cloned into a pL3-4, a defective Moloney murine leukemia virus (M-MuLV) vector. Here we show that clones selected after viral infection of mouse NIH 3T3 cells constitutively produced 128 IU/ml of human β-interferon. Constitutive synthesis of retroviral RNA was confirmed by dot blot hybridization of RNA isolated from two of the selected clones. Poly(I)·poly(C) and cycloheximide induction resulted in an increased RNA level, but this was not reflected in an increased production of biologically active interferon.", journal = "Antiviral Research", title = "Constitutive interferon expression from retroviral vector", pages = "221-215", number = "2-3", volume = "22", doi = "10.1016/0166-3542(93)90097-3" }
Knezić, Z., Nikčević, G., Marjanović, J., Glisin, V.,& Popović, Z.. (1993). Constitutive interferon expression from retroviral vector. in Antiviral Research, 22(2-3), 215-221. https://doi.org/10.1016/0166-3542(93)90097-3
Knezić Z, Nikčević G, Marjanović J, Glisin V, Popović Z. Constitutive interferon expression from retroviral vector. in Antiviral Research. 1993;22(2-3):215-221. doi:10.1016/0166-3542(93)90097-3 .
Knezić, Z., Nikčević, Gordana, Marjanović, J., Glisin, V., Popović, Z., "Constitutive interferon expression from retroviral vector" in Antiviral Research, 22, no. 2-3 (1993):215-221, https://doi.org/10.1016/0166-3542(93)90097-3 . .